Abstract
Background: A new, simple, specific and sensitive reverse-phase liquid chromatographic method was developed and validated for the estimation of felodipine (FDP) in rabbit plasma using fluorescence detector.
Materials and Methods: Plasma standard were prepared using a simple and one-step protein precipitation method and 50 μ l of the standard solutions were injected into the column. The method was found to be linear in the concentration range of 10–1000 ng/ml. The developed bioanalytical method was validated as per standard guidelines. Validation study results demonstrated accuracy, precision, sensitivity and selectivity of the proposed method.
Results: The developed method was successfully employed in the determination of FDP in plasma samples obtained from the pharmacokinetic study of the immediate release tablets in rabbits.
Keywords: Felodipine, fluorescence detector, high-performance liquid chromatography, rabbit plasma, pharmacokinetic study, oral tablet.
Current Pharmaceutical Analysis
Title:Development and Validation of a Simple High-Performance Liquid Chromatography Method for Estimation of Felodipine in Rabbit Plasma: Application to Pharmacokinetic Study
Volume: 13 Issue: 4
Author(s): Murali Monohar Pandey, Shrikant Y. Charde*, A. Jaipal, Prashant P. Raut, Puneet Goel and Love Kumar
Affiliation:
- A-005, Department of Pharmacy, Birla Institute of Technology & Science, Pilani, Hyderabad Campus, Hyderabad: 500078, Telangana,India
Keywords: Felodipine, fluorescence detector, high-performance liquid chromatography, rabbit plasma, pharmacokinetic study, oral tablet.
Abstract: Background: A new, simple, specific and sensitive reverse-phase liquid chromatographic method was developed and validated for the estimation of felodipine (FDP) in rabbit plasma using fluorescence detector.
Materials and Methods: Plasma standard were prepared using a simple and one-step protein precipitation method and 50 μ l of the standard solutions were injected into the column. The method was found to be linear in the concentration range of 10–1000 ng/ml. The developed bioanalytical method was validated as per standard guidelines. Validation study results demonstrated accuracy, precision, sensitivity and selectivity of the proposed method.
Results: The developed method was successfully employed in the determination of FDP in plasma samples obtained from the pharmacokinetic study of the immediate release tablets in rabbits.
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Cite this article as:
Monohar Pandey Murali , Y. Charde Shrikant *, Jaipal A. , P. Raut Prashant , Goel Puneet and Kumar Love , Development and Validation of a Simple High-Performance Liquid Chromatography Method for Estimation of Felodipine in Rabbit Plasma: Application to Pharmacokinetic Study, Current Pharmaceutical Analysis 2017; 13 (4) . https://dx.doi.org/10.2174/1573412912666160824120801
DOI https://dx.doi.org/10.2174/1573412912666160824120801 |
Print ISSN 1573-4129 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-676X |
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