Determination of pKa Values and Concurrent Analysis of Some Tricyclic Antidepressants in Acetonitrile-Water Mixtures

Title:Determination of pKa Values and Concurrent Analysis of Some Tricyclic Antidepressants in Acetonitrile-Water Mixtures

Volume: 13 Issue: 2

Author(s): Gulsen Guven, Senem Sanli and Nurullah Sanli

Affiliation:

Keywords: Tricyclic antidepressants, amitriptyline, clomipramine, desipramine, pK_a determination, liquid chromatography, method optimization.

Abstract: Background: In this investigation, we developed a new, rapid, novel HPLC technique for simultaneous routine analysis of amitriptyline and clomipramine. For this method, desipramine was used as an internal standard.

Experiment: Amitriptyline, clomipramine and desipramine (IS) were separated in a X-Terra RP-18 column (250mm×4.60mm ID×5µm) and DAD detector set at 210 nm. The composition of the mobile phase was acetonitrile:water 50:50 (v/v) at a flow rate of 1.0 mL min^-1. Isocratic separation was performed in less than 6 min. Additionally, pK_a values of studied compounds were evaluated by HPLC method in order to find the optimum pH value of separation. pK_a values were determined at 45 and 50% (v/v) acetonitrilewater mixtures in order to find the effect of the mobile phase composition on the ionization constants. NLREG^® program was used to calculate pK_a values of drugs. The proposed method also has some advantages such as a number of cost and time saving benefits and can be routinely used for the analysis of pharmaceutical formulations.

Result: The results show that developed method is suitable for the simultaneous analysis of these compounds in tablets without any interference from excipients.

Cite this article as:

Guven Gulsen, Sanli Senem and Sanli Nurullah, Determination of pKa Values and Concurrent Analysis of Some Tricyclic Antidepressants in Acetonitrile-Water Mixtures, Current Pharmaceutical Analysis 2017; 13 (2) . https://dx.doi.org/10.2174/1573412912666160804160912

DOI https://dx.doi.org/10.2174/1573412912666160804160912	Print ISSN 1573-4129
Publisher Name Bentham Science Publisher	Online ISSN 1875-676X