Identification of SER-PRO-CYS Peptide in Blood Serum of Multiple Sclerosis Patients

Title:Identification of SER-PRO-CYS Peptide in Blood Serum of Multiple Sclerosis Patients

Volume: 23 Issue: 9

Author(s): Severyn Myronovkij, Nazar Negrych, Tetyana Nehrych, Volodymyr Tkachenko, Serhiy Souchelnytskyi, Rostyslav Stoika and Yuriy Kit

Affiliation:

Keywords: Multiple sclerosis, blood serum, peptides.

Abstract: Monitoring of multiple sclerosis (MS) requires additional molecular markers. Recently, we used original TCA-precipitation/extraction approach in combination with MALDI TOF/TOF mass-spectrometry and identified earlier unknown 48 kDa form of the unconventional myosin IC isoform b (Myo1C) in blood serum of the MS patients. Further examination of TCA-extracted fraction of blood serum of these patients by means of thin-layer chromatography and HPLC gel-filtration allowed detecting 300-500 Da peptides. MALDI TOF/TOF massspectrometry of these peptides showed that they contain Ser-Pro-Cys amino acid sequence. We discussed potential mechanisms of a release of these peptides that were earlier unknown in blood serum of the MS patients.

Cite this article as:

Myronovkij Severyn, Negrych Nazar, Nehrych Tetyana, Tkachenko Volodymyr, Souchelnytskyi Serhiy, Stoika Rostyslav and Kit Yuriy, Identification of SER-PRO-CYS Peptide in Blood Serum of Multiple Sclerosis Patients, Protein & Peptide Letters 2016; 23 (9) . https://dx.doi.org/10.2174/0929866523666160622215628