Abstract
Objective: The aim of this study was to prepare and certify a new Certified Reference Material (CRM) of quercetin for the quality control of quercetin and related functional foods and botanical supplements.
Methods: Three different methods, including coloumetric titration (CT), differential scanning calorimetry (DSC) and mass balance (MB) methods were used in the purity determining process. Results: The purity results determined by the three methods were consistent, and the CT has the highest accuracy and the smallest uncertainty. Conclusion: CT and DSC methods were established for purity determination of quercetin for the first time. The purity of quercetin CRM, after validation and evaluation, was 99.3% ± 0.4% (k = 2, P = 0.95). The new CRM can be widely used to quantitatively analyse quercetin and other related functional foods and botanical supplements.Keywords: Quercertin, certified reference material, CT, DSC, mass balance method, functional foods.
Graphical Abstract
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