Abstract
Isotope dilution mass spectrometry (IDMS) is based on a simple and straightforward idea of using an isotope labeled compound as an internal standard for quantitation purposes. IDMS now competes with routine quantitative methods such as immunoassays (ELISA, RIA) due to its high precision, accuracy and robustness. The isotope dilution concept allows for protein quantitation and has been showing great promise in clinical application in the last decade. The present review contains a short description of the current status of proteomics and principles of absolute quantitative methodologies; it focuses on some mass spectrometric techniques, sample preparation and recent examples of IDMS applications for peptide and protein quantitation in clinical chemistry.
Keywords: Isotope dilution analysis, multi-dimensional separations, proteomics.
Current Proteomics
Title:Quantitative Proteomics with Isotope Dilution Analysis: Principles and Applications
Volume: 13 Issue: 1
Author(s): Kuanysh Kabytaev and Alexander Stoyanov
Affiliation:
Keywords: Isotope dilution analysis, multi-dimensional separations, proteomics.
Abstract: Isotope dilution mass spectrometry (IDMS) is based on a simple and straightforward idea of using an isotope labeled compound as an internal standard for quantitation purposes. IDMS now competes with routine quantitative methods such as immunoassays (ELISA, RIA) due to its high precision, accuracy and robustness. The isotope dilution concept allows for protein quantitation and has been showing great promise in clinical application in the last decade. The present review contains a short description of the current status of proteomics and principles of absolute quantitative methodologies; it focuses on some mass spectrometric techniques, sample preparation and recent examples of IDMS applications for peptide and protein quantitation in clinical chemistry.
Export Options
About this article
Cite this article as:
Kabytaev Kuanysh and Stoyanov Alexander, Quantitative Proteomics with Isotope Dilution Analysis: Principles and Applications, Current Proteomics 2016; 13 (1) . https://dx.doi.org/10.2174/1570164613666160413121219
DOI https://dx.doi.org/10.2174/1570164613666160413121219 |
Print ISSN 1570-1646 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-6247 |
- Author Guidelines
- Graphical Abstracts
- Fabricating and Stating False Information
- Research Misconduct
- Post Publication Discussions and Corrections
- Publishing Ethics and Rectitude
- Increase Visibility of Your Article
- Archiving Policies
- Peer Review Workflow
- Order Your Article Before Print
- Promote Your Article
- Manuscript Transfer Facility
- Editorial Policies
- Allegations from Whistleblowers
- Announcements
Related Articles
-
Time Recall; Future Concept of Chronomodulating Chemotherapy for Cancer
Current Pharmaceutical Biotechnology Post-Translational Modifications of PTEN and their Potential Therapeutic Implications
Current Cancer Drug Targets Red Cell Glycolytic Enzyme Disorders Caused by Mutations: An Update
Cardiovascular & Hematological Disorders-Drug Targets The Correlated Factors of Serum CA19-9 Levels in Diabetic Patients
Current Diabetes Reviews High CXCR4 Expression Correlates with Sunitinib Poor Response in Metastatic Renal Cancer
Current Cancer Drug Targets NK-1 Receptor Antagonists: A New Paradigm in Pharmacological Therapy
Current Medicinal Chemistry An Update in the Management of Obesity: The Weight of CNS Targets
Recent Patents on CNS Drug Discovery (Discontinued) Drug Uptake Systems in Liver and Kidney
Current Drug Metabolism Pathogenesis of Type 1 Diabetes: Regulation of Adhesion Molecules and Immune Cell Trafficking
Current Immunology Reviews (Discontinued) Inhibitors of HDACs - Effective Drugs Against Cancer?
Current Cancer Drug Targets Antibodies Against G-Protein Coupled Receptors: Novel Uses in Screening and Drug Development
Combinatorial Chemistry & High Throughput Screening Gynecomastia in Infants, Children, and Adolescents
Recent Patents on Endocrine, Metabolic & Immune Drug Discovery Pharmacogenetics of Target Genes Across Doxorubicin Disposition Pathway: A Review
Current Drug Metabolism Phosphodiesterase as a New Therapeutic Target for the Treatment of Spinal Cord Injury and Neurodegenerative Diseases
Current Medicinal Chemistry Targeted Therapy of the Insulin-Like Growth Factor-1 Receptor in Cancer
Combinatorial Chemistry & High Throughput Screening The PTEN/PI3K/AKT Signalling Pathway in Cancer, Therapeutic Implications
Current Cancer Drug Targets Oncogenic Fusion Tyrosine Kinases as Molecular Targets for Anti-Cancer Therapy
Anti-Cancer Agents in Medicinal Chemistry Destroying RNA as a Therapeutic Approach
Current Medicinal Chemistry Glucagon-like Peptides, the Central Nervous System, and the Regulation of Energy Homeostasis
Current Medicinal Chemistry - Central Nervous System Agents A Liver-Derived Rat Epithelial Cell Line from Biliary Origin Acquires Hepatic Functions Upon Sequential Exposure to Hepatogenic Growth Factors and Cytokines
Current Medicinal Chemistry