Generic placeholder image

Protein & Peptide Letters

Editor-in-Chief

ISSN (Print): 0929-8665
ISSN (Online): 1875-5305

Synthesis of the Peptide Ac-Wahx-KTTKS and Evaluation of the Ability to Induce In Vitro Collagen Synthesis

Author(s): Danila A.S. Guglielmi, Ariane M. Martinelli, Nathália C. Rissi, Eduardo M. Cilli, Christiane P. Soares and Leila A. Chiavacci

Volume 23, Issue 6, 2016

Page: [544 - 547] Pages: 4

DOI: 10.2174/0929866523666160331144315

Price: $65

Abstract

In this work, we report the synthesis of a peptide analogue of the KTTKS, termed Ac- Wahx-KTTKS and evaluate its cytotoxicity and role in biosynthesis of collagen for future application in skin aging. The peptide was obtained with purity higher than 97.5%. In the cytotoxicity assay, we observed non-toxic effects for Ac-WAhx-KTTKS at concentrations below 600 µM for HaCaT and 500 µM for HepG2 cells, respectively. After 24 and 48 h it was possible to observe significant changes in collagen synthesis in the groups treated with various concentrations of the peptide. In conclusion, the Ac-Wahx- KTTKS peptide increased collagen synthesis in fibroblasts by 80% and it is a promising candidate for improving skin aging.

Keywords: Ac-WAhx-KTTKS, antiaging, collagen biosynthesis, cytotoxicity, peptide synthesis, skin functions.

Graphical Abstract

Rights & Permissions Print Cite
© 2024 Bentham Science Publishers | Privacy Policy