Abstract
Background: In recent studies, we have incorporated bile acid and polyelectrolytes into pancreatic β-cell microcapsules and examined their cell viability and microcapsule morphology using various encapsulating methods.
Objective: This study aimed to incorporate 3 colloids; ultrasonic gel (USG; 1%), polystyrenic sulphate (PSS; 0.1%) and polyallylamine (PAA; 3%) and ursodeoxycholic acid (UDCA; 4%) with the polymer sodium alginate (SA; 1.2%) and the copolymer poly-L-ornithine (PLO; 1%), and using a refined vibrational jet-flow microencapsulating method, test the microcapsule properties, and cell viability without or with UDCA.
Method: The pancreatic β-cells NIT-1 were encapsulated using concentric nozzles and a refined method using voltage > 600 mv and frequency of 1750 Hz with syringe flow of 1.5 ml/min (core) and formulation solution of 2.1 ml/min, with a mixture of SA, PLO, USG, PSS and PAA without UDCA (control) or with UDCA (test). Both formulations and microcapsules were examined for surface composition and thermal and chemical biocompatibilities. The microencapsulated cells were examined for bioenergetics and production of inflammatory biomarkers. UDCA distribution within the microcapsules was also examined.
Results: Using our method, viability remained low after the addition of PSS, PAA and USG, while the incorporation of UDCA enhanced cell viability, and thermal stability was maintained.
Conclusion: Our refined microencapsulating method, when incorporating polystyrenic sulphate, polyallylamine, the gel and UDCA at 0.1:3:1:4 ratio respectively, produced stable microcapsules suggesting potential applications in cell microencapsulation and diabetes treatment.
Keywords: Diabetes, artificial cell microencapsulation, NIT-1 cells, bile acid, biomaterials.
Current Diabetes Reviews
Title:The Effects of Ionic Gelation- Vibrational Jet Flow Technique in Fabrication of Microcapsules Incorporating β-cell: Applications in Diabetes
Volume: 13 Issue: 1
Author(s): Armin Mooranian, Rebecca Negrulj and Hani Al-Salami
Affiliation:
Keywords: Diabetes, artificial cell microencapsulation, NIT-1 cells, bile acid, biomaterials.
Abstract: Background: In recent studies, we have incorporated bile acid and polyelectrolytes into pancreatic β-cell microcapsules and examined their cell viability and microcapsule morphology using various encapsulating methods.
Objective: This study aimed to incorporate 3 colloids; ultrasonic gel (USG; 1%), polystyrenic sulphate (PSS; 0.1%) and polyallylamine (PAA; 3%) and ursodeoxycholic acid (UDCA; 4%) with the polymer sodium alginate (SA; 1.2%) and the copolymer poly-L-ornithine (PLO; 1%), and using a refined vibrational jet-flow microencapsulating method, test the microcapsule properties, and cell viability without or with UDCA.
Method: The pancreatic β-cells NIT-1 were encapsulated using concentric nozzles and a refined method using voltage > 600 mv and frequency of 1750 Hz with syringe flow of 1.5 ml/min (core) and formulation solution of 2.1 ml/min, with a mixture of SA, PLO, USG, PSS and PAA without UDCA (control) or with UDCA (test). Both formulations and microcapsules were examined for surface composition and thermal and chemical biocompatibilities. The microencapsulated cells were examined for bioenergetics and production of inflammatory biomarkers. UDCA distribution within the microcapsules was also examined.
Results: Using our method, viability remained low after the addition of PSS, PAA and USG, while the incorporation of UDCA enhanced cell viability, and thermal stability was maintained.
Conclusion: Our refined microencapsulating method, when incorporating polystyrenic sulphate, polyallylamine, the gel and UDCA at 0.1:3:1:4 ratio respectively, produced stable microcapsules suggesting potential applications in cell microencapsulation and diabetes treatment.
Export Options
About this article
Cite this article as:
Mooranian Armin, Negrulj Rebecca and Al-Salami Hani, The Effects of Ionic Gelation- Vibrational Jet Flow Technique in Fabrication of Microcapsules Incorporating β-cell: Applications in Diabetes, Current Diabetes Reviews 2017; 13 (1) . https://dx.doi.org/10.2174/1573399812666151229101756
DOI https://dx.doi.org/10.2174/1573399812666151229101756 |
Print ISSN 1573-3998 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-6417 |
Call for Papers in Thematic Issues
Advancing Diabetic Wound Healing: Mechanisms and Interventions
In recent years, diabetic wounds have become a global health concern with the increase in the incidence of diabetes. Diabetic wounds are a kind of chronic and refractory ulcer. It is generally due to the microcirculatory disturbances and the reduced levels of endogenous growth factors. Delayed cutaneous wound healing is ...read more
Oxidative and inflammatory responses in the development of secondary diabetic complications
Diabetes, along with its associated secondary complications, represents a significant global health challenge, contributing significantly to morbidity and mortality. Unhealthy lifestyle habits, reduced physical activity, environmental pollutants, and stress are pivotal factors in the onset of diabetes, particularly type-2 diabetes. Poorly managed hyperglycemia can lead to various complications, including neuropathy, ...read more
- Author Guidelines
- Graphical Abstracts
- Fabricating and Stating False Information
- Research Misconduct
- Post Publication Discussions and Corrections
- Publishing Ethics and Rectitude
- Increase Visibility of Your Article
- Archiving Policies
- Peer Review Workflow
- Order Your Article Before Print
- Promote Your Article
- Manuscript Transfer Facility
- Editorial Policies
- Allegations from Whistleblowers
- Announcements
Related Articles
-
Recent Advancements in Diagnosis and Therapy of Liver Cirrhosis
Current Drug Targets Long Noncoding RNAs as Diagnostic and Therapeutic Targets for Ischemic Stroke
Current Pharmaceutical Design Co-morbidity of Diabetes Mellitus and Orodental Diseases in Nigeria
Current Diabetes Reviews The Impact of Diabetes Mellitus on Coronary Artery Disease: New Therapeutic Approaches
Current Pharmaceutical Design Editorial
Current Hypertension Reviews Moving to the Rhythm with Clock (Circadian) Genes, Autophagy, mTOR, and SIRT1 in Degenerative Disease and Cancer
Current Neurovascular Research Molecular Imaging and Targeted Drug Delivery Using Albumin-Based Nanoparticles
Current Pharmaceutical Design Evaluation of the Possible Contribution of Antioxidants Administration in Metabolic Syndrome
Current Pharmaceutical Design Drug Hypersensitivity: Epidemiology and Risk Factors
Current Pharmaceutical Design Islet Amyloid Polypeptide and Diabetes
Current Protein & Peptide Science Molecular Links Between Diabetes and Osteoarthritis: The Role of Physical Activity
Current Diabetes Reviews The Molecular Basis for the Pharmacological Activity of Anthocyans
Current Medicinal Chemistry Endless Pursuit: Evolution of Insulin Administration
Current Pharmaceutical Biotechnology Editorial [Hot topic:The Two Faces of High-Density Lipoprotein (Executive Editor: Sophie Van Linthout)]
Current Pharmaceutical Design Cardiovascular Health vs Cardiovascular Risk A 2011 Update: Cardiovascular Risk Stratification, the Basis to Reach Therapeutic Goals
Current Hypertension Reviews 30 Years Lost in Anesthesia Theory
Cardiovascular & Hematological Agents in Medicinal Chemistry Environmental (Lifestyle) Risk Factors for LADA
Current Diabetes Reviews Serum Carotenoids and Risks of Diabetes and Diabetic Retinopathy in a Chinese Population Sample
Current Molecular Medicine Do Rheumatoid Arthritis Patients Rate their Health Status Different than their Caregivers?
Anti-Inflammatory & Anti-Allergy Agents in Medicinal Chemistry Nutrigenetics, Metabolic Syndrome Risk and Personalized Nutrition
Current Vascular Pharmacology