Crocin Rich Extract of Nyctanthes arbor-tristis Flower Calyx Induces Anti-angiogenic Activity

Author(s): Neha D.Pawar, Shital S.Panchal, Kumaravelu J., Tasneem Shahmalak, Chhaya Gadgoli.

Journal Name: The Natural Products Journal

Volume 6 , Issue 1 , 2016

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Abstract:

Crocin is an apocarotenoid present in Nyctanthes arbortrisits (NAT) flower calyx extract in abundant quantity of about 36.7% w/w. The carotenoid rich extract of NAT exhibited dose dependant anti-angiogenic effect in chick-chorioallantoic membrane (CAM) assay. The anti-angiogenic activity of NAT extract (1 mg/ml) was observed to be equivalent to Heparin (1 mg/ml) with the IC50 value of 0.5 mg/ml. The antiangiogenic activity of NAT was further confirmed by in-vivo Matrigel Matrix model of Balb/c mice with VEGF inhibitor, sunitinib malate (1 μM) as the anti-angiogenic control. The in-vivo study revealed significant (P<0.01) reduction in hemoglobin levels and inhibited angiogenesis as compared to angiogenic control viz. VEGF loaded matrigel matrix. The anti-angiogenic activity of NAT calyx ethanolic extract is likely to be due to the presence of Crocin since carotenoids possess inherent strong anti-oxidant potential. Our result brings out a new naturally occurring anti-angiogenic agent which may find its utility in the treatment of conditions like cancer, diabetic retinopathy and cataract.

Keywords: Nyctanthes arbourtristis, carotenoids, CAM, anti-angiogenic, matrigel matrix, crocin.

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Article Details

VOLUME: 6
ISSUE: 1
Year: 2016
Page: [40 - 48]
Pages: 9
DOI: 10.2174/2210315506666151208212552
Price: $58

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