Comparative Two-Dimensional Gel Electrophoresis of Trypanosoma cruzi Mammalian-Stage Forms in an Alkaline pH Range

Title:Comparative Two-Dimensional Gel Electrophoresis of Trypanosoma cruzi Mammalian-Stage Forms in an Alkaline pH Range

Volume: 22 Issue: 12

Author(s): Adriana D. Magalhães, Rayner Myr L. Queiroz, Izabela M. D. Bastos, Jaime M. Santana, Marcelo V. Sousa, Carlos André O. Ricart and Sébastien Charneau

Affiliation:

Keywords: Alkaline 2-DE, amastigote, Chagas disease, proteome, Trypanosoma cruzi, trypomastigote.

Abstract: It is estimated that several million people are currently infected worldwide by the protozoan parasite, Trypanosoma cruzi, which causes Chagas disease. After mammalian host infection, a fundamental event is the differentiation from infective trypomastigotes into replicative amastigotes (amastigogenesis) inside host-cells. To unravel the particularities of both forms, it is essential to identify molecules presented in each form. Since T. cruzi gene expression regulation occurs mainly at posttranscriptional level, a proteomic approach is appropriate. Due to intrinsic difficulties with performing 2-DE in the alkaline pH range, there are no reports on 2-DE-based comparative proteome analysis of T. cruzi mammalianstage forms that focus on alkaline polypeptides. Here, we performed a comparative proteome analysis between tissue culture- derived trypomastigotes and extracellular amastigote-like cells using conditions optimized for the 6-11 pH range followed by identification by MALDI-TOF/TOF technology. The alkaline 2-DE maps from both forms show that proteins with a pI above 7.0 were not underrepresented (= 65% of proteins detected). Moreover the differences in protein expression between the Human-hosted T. cruzi forms corroborated previous proteomic studies and corresponded to their biological traits.

Cite this article as:

Magalhães D. Adriana, Queiroz Myr L. Rayner, Bastos M. D. Izabela, Santana M. Jaime, Sousa V. Marcelo, Ricart André O. Carlos and Charneau Sébastien, Comparative Two-Dimensional Gel Electrophoresis of Trypanosoma cruzi Mammalian-Stage Forms in an Alkaline pH Range, Protein & Peptide Letters 2015; 22 (12) . https://dx.doi.org/10.2174/0929866522666150915122120