Abstract
This review introduces the concept of enzyme biosensors. As the major feature of an enzyme is its selectivity and specificity, we briefly highlighted the principles of enzyme production and enzyme classification. Moreover, enzyme immobilization methods, including physical adsorption and entrapment techniques with special attention on cross-linking and covalent binding are discussed followed by different electrochemical methods used in the biosensors fabrication. Furthermore, a comprehensive discussion about horseradish peroxidase (HRP) as a model enzyme is presented with investigation of some modified enzyme electrodes such as screen-printed and carbon paste electrodes. Some approaches to enhance biosensors performances and their applications are also highlighted.
Keywords: Biosensors, enzyme, electrochemistry, immobilization techniques, modified electrodes.
Current Analytical Chemistry
Title:Recent Advances on Electrochemical Enzyme Biosensors
Volume: 12 Issue: 1
Author(s): Ezzaldeen Younes Jomma and Shou-Nian Ding
Affiliation:
Keywords: Biosensors, enzyme, electrochemistry, immobilization techniques, modified electrodes.
Abstract: This review introduces the concept of enzyme biosensors. As the major feature of an enzyme is its selectivity and specificity, we briefly highlighted the principles of enzyme production and enzyme classification. Moreover, enzyme immobilization methods, including physical adsorption and entrapment techniques with special attention on cross-linking and covalent binding are discussed followed by different electrochemical methods used in the biosensors fabrication. Furthermore, a comprehensive discussion about horseradish peroxidase (HRP) as a model enzyme is presented with investigation of some modified enzyme electrodes such as screen-printed and carbon paste electrodes. Some approaches to enhance biosensors performances and their applications are also highlighted.
Export Options
About this article
Cite this article as:
Jomma Younes Ezzaldeen and Ding Shou-Nian, Recent Advances on Electrochemical Enzyme Biosensors, Current Analytical Chemistry 2016; 12 (1) . https://dx.doi.org/10.2174/1573411011666150611184215
DOI https://dx.doi.org/10.2174/1573411011666150611184215 |
Print ISSN 1573-4110 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-6727 |
Call for Papers in Thematic Issues
Advancements in biomedicine, corrosion inhibition/monitoring, and lubrication of carbon dots
Carbon dots, a class of carbon-based nanomaterials, have garnered substantial interest in various scientific and engineering fields due to their unique properties, including excellent optical properties, outstanding biocompatibility, easy preparation, good dispersity, ultrasmall size, low toxicity and environmental friendliness. This special issue of Current Analytical Chemistry aims to showcase cutting-edge ...read more
Analytical methods for environmental and food analysis
In recent years, the study of the chemical composition of food and the environment depends on analytical methods in order to acquire information about the chemical composition, processing, quality control and contamination of soil, water, air, plants and foods, aspects very important for food safety and environmental quality control. There ...read more
Applications of artificial intelligence in chemical analysis
Artificial intelligence (AI) has demonstrated its versatility in almost all scientific fields, especially analytical chemistry, which is leading the way in the integration of AI. According to Chemical Abstract Services, one of the major disciplines contributing to AI-related publications and patents is analytical chemistry. That?s because techniques such as deep ...read more
Bioanalytical and Biosensor Technologies for Target Molecules
Bioanalytical and biosensor technologies constitute the core of detecting and sensing diverse molecules within living organisms, encompassing proteins, DNA, small molecular metabolites, and beyond. These molecules assume pivotal roles in biological processes, rendering their analysis and sensing indispensable for comprehending biological phenomena and unraveling disease mechanisms. In the current landscape ...read more
- Author Guidelines
- Graphical Abstracts
- Fabricating and Stating False Information
- Research Misconduct
- Post Publication Discussions and Corrections
- Publishing Ethics and Rectitude
- Increase Visibility of Your Article
- Archiving Policies
- Peer Review Workflow
- Order Your Article Before Print
- Promote Your Article
- Manuscript Transfer Facility
- Editorial Policies
- Allegations from Whistleblowers
Related Articles
-
Tamoxifen and its New Derivatives in Cancer Research
Recent Patents on Anti-Cancer Drug Discovery Menopause and Non-Alcoholic Fatty Liver Disease: A Review Focusing on Therapeutic Perspectives
Current Vascular Pharmacology Acute Fulminant Hepatatis in Kidney Transplant Recipient After Repeated Sevoflurane Anesthesia - A Case Report and Literature Review
Current Drug Safety The Neuropharmacology of Implicit Learning
Current Neuropharmacology Surface-Linked Liposomal Antigen Induces IgE Selective Unresponsiveness in a T-Cell Independent Fashion
Current Drug Targets - Immune, Endocrine & Metabolic Disorders Oncogenic Fusion Tyrosine Kinases as Molecular Targets for Anti-Cancer Therapy
Anti-Cancer Agents in Medicinal Chemistry Maintaining Plasma Fibrinogen Levels and Fibrinogen Replacement Therapies for Treatment of Intracranial Hemorrhage
Current Drug Targets Effects of Dietary Polyunsaturated Fatty Acids on Mitochondria
Current Pharmaceutical Design Cyclodextrins in Nitrosation Chemistry: New Insights of the NO-Transfer Processes
Mini-Reviews in Organic Chemistry A Proposal----The Renal Kallikrein-Kinin System as a Compensating System for Salt Accumulation after Excess Salt Intake
Current Hypertension Reviews Current Status of CETP Inhibitors in the Treatment of Hyperlipidemia: An Update
Current Clinical Pharmacology Autoimmunity and Apoptosis - Therapeutic Implications
Current Medicinal Chemistry Incretin-Based Therapies, Glucometabolic Health and Endovascular Inflammation
Current Pharmaceutical Design Assessment of Chemicals Applying Partial Order Ranking Techniques
Combinatorial Chemistry & High Throughput Screening Atypical Antipsychotic Drugs During Pregnancy. Their Effects on the Mother and Offspring
Current Women`s Health Reviews Perioperative Glycemic Management in Insulin Pump Patients Undergoing Noncardiac Surgery
Current Pharmaceutical Design Apolipoprotein B and Apolipopotein A-I in Vascular Risk Prediction-A Review
Current Pharmaceutical Design Objectively Assessed Physical Activity and Sedentary Behaviour During Pregnancy in Portuguese Women: Differences Between Trimesters and Weekdays and Weekends
Current Women`s Health Reviews Getting an Insight into the Complexity of Major Chronic Inflammatory and Degenerative Diseases: A Potential New Systemic Approach to Their Treatment
Current Pharmaceutical Biotechnology Fat Tissue Distribution Changes in HIV-Infected Patients Treated with Lopinavir/Ritonavir. Results of the MONARK Trial
Current HIV Research