Abstract
MicroRNAs have been discovered as regulators of gene expression and thus their potential in clinical disease diagnostics, prognosis and therapy is being actively pursued. MicroRNAs play an important role in atherosclerosis-related diseases, such as cerebrovascular and cardiovascular disease. However, the effect of miR-185 and miR-146a on patients with ischemic stroke (IS) in the different phases has not been reported. In this study, we investigated the amounts of three miRNAs, miR-185, miR-146a, and miR-145 in blood circulation of IS patients. We enrolled 60 patients with IS in the acute or sub-acute phase and 30 healthy controls. We divided the patients into two groups, patients with ischemic stroke in the acute phase (ISA) and patients with ischemic stroke in the subacute phase (ISS). We measured circulating miRNAs expression by miRNA microarray and real-time polymerase chain reaction (PCR) analysis. Testing by miRNA microarray and RT-PCR analyses showed that miR-145 levels in healthy subjects were similar to patients with IS, whereas miR-146a and miR-185 were present with quite low abundance in ISA compared with healthy individuals; moreover, we found that miR-146a levels were downregulated in ISA but upregulated in ISS which may help provide new insights into the diagnosis and therapy of IS.
Keywords: MicroRNAs, blood, MiR-185, MiR-146a, ischemic stroke, phase.
Current Neurovascular Research
Title:Differential Regulation of microRNAs in Patients with Ischemic Stroke
Volume: 12 Issue: 3
Author(s): Sheng-Hua Li, Sheng-You Su and Jing-Li Liu
Affiliation:
Keywords: MicroRNAs, blood, MiR-185, MiR-146a, ischemic stroke, phase.
Abstract: MicroRNAs have been discovered as regulators of gene expression and thus their potential in clinical disease diagnostics, prognosis and therapy is being actively pursued. MicroRNAs play an important role in atherosclerosis-related diseases, such as cerebrovascular and cardiovascular disease. However, the effect of miR-185 and miR-146a on patients with ischemic stroke (IS) in the different phases has not been reported. In this study, we investigated the amounts of three miRNAs, miR-185, miR-146a, and miR-145 in blood circulation of IS patients. We enrolled 60 patients with IS in the acute or sub-acute phase and 30 healthy controls. We divided the patients into two groups, patients with ischemic stroke in the acute phase (ISA) and patients with ischemic stroke in the subacute phase (ISS). We measured circulating miRNAs expression by miRNA microarray and real-time polymerase chain reaction (PCR) analysis. Testing by miRNA microarray and RT-PCR analyses showed that miR-145 levels in healthy subjects were similar to patients with IS, whereas miR-146a and miR-185 were present with quite low abundance in ISA compared with healthy individuals; moreover, we found that miR-146a levels were downregulated in ISA but upregulated in ISS which may help provide new insights into the diagnosis and therapy of IS.
Export Options
About this article
Cite this article as:
Li Sheng-Hua, Su Sheng-You and Liu Jing-Li, Differential Regulation of microRNAs in Patients with Ischemic Stroke, Current Neurovascular Research 2015; 12 (3) . https://dx.doi.org/10.2174/1567202612666150605121709
DOI https://dx.doi.org/10.2174/1567202612666150605121709 |
Print ISSN 1567-2026 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-5739 |
- Author Guidelines
- Graphical Abstracts
- Fabricating and Stating False Information
- Research Misconduct
- Post Publication Discussions and Corrections
- Publishing Ethics and Rectitude
- Increase Visibility of Your Article
- Archiving Policies
- Peer Review Workflow
- Order Your Article Before Print
- Promote Your Article
- Manuscript Transfer Facility
- Editorial Policies
- Allegations from Whistleblowers
Related Articles
-
Preface
Current Pharmaceutical Design Heart Failure in Minority Populations - Impediments to Optimal Treatment in Australian Aborigines
Current Cardiology Reviews Editorial [Hot Topic: Matrix Metalloproteinases in Cardiovascular Disease (Guest Editors: N. Papageorgiou & D. Tousoulis)]
Current Topics in Medicinal Chemistry Acute Coronary Syndromes: A Role of Immune System
Anti-Inflammatory & Anti-Allergy Agents in Medicinal Chemistry Acute Cerebral Blood Flow Variations after Human Cardiac Arrest Assessed by Stable Xenon Enhanced Computed Tomography
Current Neurovascular Research Should Adipokines be Considered in the Choice of the Treatment of Obesity-Related Health Problems?
Current Drug Targets Herbal Folklore Medication for Liver Disorders
Current Traditional Medicine Varicella-Zoster Virus Infections During Pregnancy: Epidemiology, Clinical Symptoms, Diagnosis, Prevention and Therapy
Current Pediatric Reviews Discharge Interventions and Modifiable Risk Factors for Preventing Hospital Readmissions in Children with Medical Complexity
Reviews on Recent Clinical Trials The Effects of Pemafibrate in Japanese Patients with Type 2 Diabetes Receiving HMG-CoA Reductase Inhibitors
Endocrine, Metabolic & Immune Disorders - Drug Targets Poly(ADP)-Ribose Polymerase-1 Inhibitors as a Potential Treatment for Cocaine Addiction
CNS & Neurological Disorders - Drug Targets Unfolded Protein Response as a Therapeutic Target in Cardiovascular Disease
Current Topics in Medicinal Chemistry Biomarkers of Chronic Kidney Disease - Mineral and Bone Disorder (CKD-MBD), A Review of Published Patents and Patent Applications
Recent Patents on Biomarkers Astrocytes: Targets for Neuroprotection in Stroke
Central Nervous System Agents in Medicinal Chemistry Are Cerebrovascular White Matter Lesions an Early Sign of Vascular Cognitive Impairment and Vascular Dementia?
Vascular Disease Prevention (Discontinued) Physiological Significance and Therapeutic Potential of Adrenomedullin in Pulmonary Hypertension
Cardiovascular & Hematological Disorders-Drug Targets Bioinformatics Analysis of Functional Protein Sequences Reveals a Role for Tumor Necrosis Factor-α and Nitric Oxide in Insulin Resistance Syndrome
Current Nutrition & Food Science Role of Statins in Diabetes Complications
Current Diabetes Reviews Novel Drug-Induced Pulmonary Complications in Cancer Patients You Can Save Life!
Current Respiratory Medicine Reviews Predictors of Left Ventricular Ejection Fraction Improvement after Radiofrequency Catheter Ablation in Patients with PVC-Induced Cardiomyopathy: A Systematic Review
Current Cardiology Reviews