Abstract
The aim of the present study was to examine the impact of a four platinum complexes of formula [Pt2L4(berenil)2]Cl4 where L is 3-ethylpyridine (Pt10), 3-(n-butyl)pyridine (Pt11), 4- ethylpyridine (Pt12) and 4-(t-butyl)pyridine (Pt13) on viability of Ishikawa endometrial cancer cells using the MTT assay and inhibition of [3H]thymidine incorporation into DNA. Our results confirm that compounds Pt10-Pt13 are more potent antiproliferative agents than cisplatin in endometrial cancer cells. Moreover, it was shown that all examined compounds Pt10-Pt13 inhibit collagen biosynthesis in neoplastic cells stronger than cisplatin. Flow cytometric analysis after annexin V-FITC and propidium iodide staining confirmed also that apoptosis was the main response of Ishikawa endometrial cancer cells to Pt10-Pt13 treatment. Our results suggest that apoptosis of Ishikawa endometrial cancer cell lines in the presence of Pt10-Pt13 follows the mitochondrial pathway, with the decrease in mitochondrial membrane potential and activation of caspase 9, as well as by the external pathway with the significant increase in FADD protein expression and caspase 8.
Keywords: Apoptosis, cisplatin, cytotoxicity, dinuclear platinum complexes, Ishikawa endometrial cancer, flow cytometry.
Medicinal Chemistry
Title:Effects of Novel Alkyl Pyridine Platinum Complexes on Apoptosis in Ishikawa Endometrial Cancer Cells
Volume: 11 Issue: 6
Author(s): Robert Czarnomysy, Anna Bielawska, Anna Muszyńska and Krzysztof Bielawski
Affiliation:
Keywords: Apoptosis, cisplatin, cytotoxicity, dinuclear platinum complexes, Ishikawa endometrial cancer, flow cytometry.
Abstract: The aim of the present study was to examine the impact of a four platinum complexes of formula [Pt2L4(berenil)2]Cl4 where L is 3-ethylpyridine (Pt10), 3-(n-butyl)pyridine (Pt11), 4- ethylpyridine (Pt12) and 4-(t-butyl)pyridine (Pt13) on viability of Ishikawa endometrial cancer cells using the MTT assay and inhibition of [3H]thymidine incorporation into DNA. Our results confirm that compounds Pt10-Pt13 are more potent antiproliferative agents than cisplatin in endometrial cancer cells. Moreover, it was shown that all examined compounds Pt10-Pt13 inhibit collagen biosynthesis in neoplastic cells stronger than cisplatin. Flow cytometric analysis after annexin V-FITC and propidium iodide staining confirmed also that apoptosis was the main response of Ishikawa endometrial cancer cells to Pt10-Pt13 treatment. Our results suggest that apoptosis of Ishikawa endometrial cancer cell lines in the presence of Pt10-Pt13 follows the mitochondrial pathway, with the decrease in mitochondrial membrane potential and activation of caspase 9, as well as by the external pathway with the significant increase in FADD protein expression and caspase 8.
Export Options
About this article
Cite this article as:
Czarnomysy Robert, Bielawska Anna, Muszyńska Anna and Bielawski Krzysztof, Effects of Novel Alkyl Pyridine Platinum Complexes on Apoptosis in Ishikawa Endometrial Cancer Cells, Medicinal Chemistry 2015; 11 (6) . https://dx.doi.org/10.2174/1573406411666150206163547
DOI https://dx.doi.org/10.2174/1573406411666150206163547 |
Print ISSN 1573-4064 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-6638 |
Call for Papers in Thematic Issues
Carbohydrates in Computational and Medicinal Chemistry
Carbohydrates are the most essential organic molecules and are involved in the maintenance of various physiological and metabolic processes in living organisms. Carbohydrate-based compounds have come to the attention of researchers because of their significant contributions to biological functions, such as cell development and cell proliferation, connections between several cells, ...read more
Recent Advances in the Medicinal Chemistry of Cancer
Scope of the Thematic Issue: Correlation between structure and function is one of the important aspects of the success of anti-cancer compounds associated with their structure-activity interactions, physiology, biochemical, molecular, and genetic processes. Overcoming these obstacles is key to obtaining further insights into developments in rational drug design, bioorganic chemistry, ...read more
- Author Guidelines
- Graphical Abstracts
- Fabricating and Stating False Information
- Research Misconduct
- Post Publication Discussions and Corrections
- Publishing Ethics and Rectitude
- Increase Visibility of Your Article
- Archiving Policies
- Peer Review Workflow
- Order Your Article Before Print
- Promote Your Article
- Manuscript Transfer Facility
- Editorial Policies
- Allegations from Whistleblowers
Related Articles
-
Biological Modulation by Lectins and Their Ligands in Tumor Progression and Metastasis
Anti-Cancer Agents in Medicinal Chemistry Familial Colorectal Cancer Type X
Current Genomics Subject Index to Volume 4
Current Cancer Drug Targets Modulation of Gene Transcription by Natural Products - A Viable Anticancer Strategy
Current Pharmaceutical Design Mitochondrial Drug Targets in Cell Death and Cancer
Current Pharmaceutical Design Anlotinib Overcomes Multiple Drug Resistant Colorectal Cancer Cells via Inactivating PI3K/AKT Pathway
Anti-Cancer Agents in Medicinal Chemistry Protein Phosphatase 1 and Its Complexes in Carcinogenesis
Current Cancer Drug Targets Breast Cancer: Biological Characteristics in Postmenopausal Type 2 Diabetic Women. Identification of Therapeutic Targets
Current Drug Targets - Immune, Endocrine & Metabolic Disorders Inhibitory Smad7: Emerging Roles in Health and Disease
Current Molecular Pharmacology Factors Promoting Tamoxifen Resistance in Breast Cancer via Stimulating Breast Cancer Stem Cell Expansion
Current Medicinal Chemistry Modifier Gene Studies to Identify New Therapeutic Targets in Cystic Fibrosis
Current Pharmaceutical Design Quinone Methide Bioactivation Pathway: Contribution to Toxicity and/or Cytoprotection?
Current Organic Chemistry Inhibitory Effects of Bisphosphonates on the Proliferation of Human Ovarian Cancer Cell Lines and the Mechanism
Medicinal Chemistry Editorial [Hot Topic: Steroid Nuclear Receptor Family (Guest Editor: Pedro H.H. Hermkens)]
Current Medicinal Chemistry Meet Our Editorial Board Member
Current Drug Targets Alternative Approaches to the Discovery and Development of Telomerase- Targeted Anticancer Drugs
Mini-Reviews in Medicinal Chemistry Targeted Tumor Diagnosis and Therapy with Peptide Hormones as Radiopharmaceuticals
Anti-Cancer Agents in Medicinal Chemistry Rho GTPase Effector Functions in Tumor Cell Invasion and Metastasis
Current Drug Targets HSP90 Inhibitors: Current Development and Potential in Cancer Therapy
Recent Patents on Anti-Cancer Drug Discovery PET-MRI Based Molecular Imaging as a Response Marker in Cervical Cancer: A Systematic Review
Current Molecular Imaging (Discontinued)