Lipopolysaccharide-Induced Chorioamnionitis Causes Acute Inflammatory Changes in the Ovine Central Nervous System

Author(s): Eveline Strackx, Michelle A.R. Sparnaaij, Evi Vlassaks, Reint Jellema, Elke Kuypers, Johan S.H. Vles, Boris W. Kramer, Antonio W.D. Gavilanes.

Journal Name: CNS & Neurological Disorders - Drug Targets

Volume 14 , Issue 1 , 2015

Abstract:

Objective: To better understand the inflammatory response in the central nervous system (CNS) after lipopolysaccharide (LPS)-induced chorioamnionitis.

Study Design: Fetal sheep were exposed to intra-amniotic LPS 2 or 14 days before preterm delivery at 125 days of gestation. mRNA levels of cytokines, TLRs and anti-oxidants were determined in different CNS regions.

Results: Interleukin 1β levels increased in hippocampus, cortex and cerebellum 2 days after LPS exposure, while Interleukin 8 levels increased in the periventricular white matter as well. Levels returned back to control levels after 14 days. Tumor necrosis factor-α levels increased in hippocampus and cortex after 2 days. Toll like receptor 4 levels was upregulated in all grey matter regions 2 and 14 days after exposure. Glutathione s-transferase mRNA levels were lower after 2 and 14 days in all grey matter regions.

Conclusion: Intra-amniotic LPS exposure causes acute and region-specific changes in inflammatory markers in the fetal brain, with grey matter being more affected than white matter.

Condensation: Intra-amniotic LPS exposure causes acute and region-specific changes in cytokines, TLR and anti-oxidants levels, with grey matter being more affected than white matter.

Keywords: Brain, cytokines, lipopolysaccharide-induced chorioamnionitis, sheep, toll like receptor.

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Article Details

VOLUME: 14
ISSUE: 1
Year: 2015
Page: [77 - 84]
Pages: 8
DOI: 10.2174/1871527314666150116120029

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