Abstract
We described herein a simple and efficient microwave assisted synthesis of HYNIC analogues. Two different activated esters of HYNIC, the hydrazine protected with a trifluoroacetyl group (5) and the free hydrazine (6) were conjugated to the monoclonal antibody Nimotuzumab. Technetium-99m radiolabeling of Nimotuzumab was achieved with high efficiency using 5 and 6 derivates. The NHS-HYNIC-Tfa derivate allowed better labeling yields during longer times of preservation of the conjugated antibody.
Keywords: 99m-technetium, HYNIC, microwave-assisted, nimotuzumab, trifluoroacetyl.
Current Radiopharmaceuticals
Title:Microwave-assisted Synthesis of HYNIC Protected Analogue for 99mTc Labeled Antibody
Volume: 7 Issue: 2
Author(s): Maria F. Garcia, Victoria Calzada, Ximena Camacho, Enzo Goicochea, Juan Pablo Gambini, Thomas P. Quinn, Williams Porcal and Pablo Cabral
Affiliation:
Keywords: 99m-technetium, HYNIC, microwave-assisted, nimotuzumab, trifluoroacetyl.
Abstract: We described herein a simple and efficient microwave assisted synthesis of HYNIC analogues. Two different activated esters of HYNIC, the hydrazine protected with a trifluoroacetyl group (5) and the free hydrazine (6) were conjugated to the monoclonal antibody Nimotuzumab. Technetium-99m radiolabeling of Nimotuzumab was achieved with high efficiency using 5 and 6 derivates. The NHS-HYNIC-Tfa derivate allowed better labeling yields during longer times of preservation of the conjugated antibody.
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Cite this article as:
Garcia Maria F., Calzada Victoria, Camacho Ximena, Goicochea Enzo, Gambini Pablo Juan, Quinn P. Thomas, Porcal Williams and Cabral Pablo, Microwave-assisted Synthesis of HYNIC Protected Analogue for 99mTc Labeled Antibody, Current Radiopharmaceuticals 2014; 7 (2) . https://dx.doi.org/10.2174/1874471007666141128160449
DOI https://dx.doi.org/10.2174/1874471007666141128160449 |
Print ISSN 1874-4710 |
Publisher Name Bentham Science Publisher |
Online ISSN 1874-4729 |
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