A Comparison of Immobilization of Commercial and Bacterial Lipase on Silica Matrix

Title:A Comparison of Immobilization of Commercial and Bacterial Lipase on Silica Matrix

Volume: 3 Issue: 4

Author(s): Nitin Kumar Saun, Swati Sharma, Rajni Thakur and Reena Gupta

Affiliation:

Keywords: Bacterial lipase, commercial lipase, glutaraldehyde, silica matrix, surface adsorption.

Abstract: The aim of the study was to compare the optimum immobilization conditions of lipase obtained from thermophilic Bacillus aerius and commercial lipase ‘Lipolase’. Both bacterial and commercial lipase were immobilized by surface adsorption onto silica matrix (100-200μm mesh with pore size 2.4 nm) and subsequently exposed to 4% glutaraldehyde, showed binding efficiency of 96.25% and 75.64%, respectively. Maximum enzyme loading was attained after 2 h of incubation in each case. Comparison of various independent variables like reaction time, temperature, substrate affinity, substrate concentration, buffer pH, reusability and thermostability was done to study the effect of matrix on the activity of both bacterial and commercial lipase. Bacterial lipase showed maximum activity with para-Nitrophenyl palmitate (20 mM) at pH 9.5, incubation time of 10 min at 55°C. On the other hand, commercial enzyme showed maximum activity with para-Nitrophenyl palmitate (20 mM) at pH 8.5, incubation time of 10 min and at temperature 40°C. All the metal ions studied exert an inhibitory effect on both bacterial and commercial lipase.

Cite this article as:

Saun Kumar Nitin, Sharma Swati, Thakur Rajni and Gupta Reena, A Comparison of Immobilization of Commercial and Bacterial Lipase on Silica Matrix, Current Biotechnology 2014; 3 (4) . https://dx.doi.org/10.2174/2211550103999141121104849