Abstract
Induced pluripotent stem (iPS) cells were created from mouse fibroblasts by induced expression of Yamanaka factors, Oct3/4, Sox2, Klf4, and c-Myc. This technique has quickly resulted in an exponential increase in the amount of pluripotency studies, and has provided a valuable tool in regenerative medicine. At the same time, many methodologies to generate iPS cells have been reported, and are comprised mainly of viral methods and non-viral methods. Although viral methods may not be applicable for clinical applications, various nonviral methods have been reported in recent years, including DNA vector-based approaches, transfection of mRNA, transduction of reprogramming proteins, and use of small molecule compounds. This review summarizes and evaluates these non-viral methods.
Keywords: DNA integration-free, induced pluripotent stem cells, non-viral methods.
Current Stem Cell Research & Therapy
Title:Non-Viral Methods For Generating Integration-Free, Induced Pluripotent Stem Cells
Volume: 10 Issue: 2
Author(s): Xiao-Yue Deng, Hu Wang, Tao Wang, Xian-Tao Fang, Li-Li Zou, Zhi-Ying Li and Chang-Bai Liu
Affiliation:
Keywords: DNA integration-free, induced pluripotent stem cells, non-viral methods.
Abstract: Induced pluripotent stem (iPS) cells were created from mouse fibroblasts by induced expression of Yamanaka factors, Oct3/4, Sox2, Klf4, and c-Myc. This technique has quickly resulted in an exponential increase in the amount of pluripotency studies, and has provided a valuable tool in regenerative medicine. At the same time, many methodologies to generate iPS cells have been reported, and are comprised mainly of viral methods and non-viral methods. Although viral methods may not be applicable for clinical applications, various nonviral methods have been reported in recent years, including DNA vector-based approaches, transfection of mRNA, transduction of reprogramming proteins, and use of small molecule compounds. This review summarizes and evaluates these non-viral methods.
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Cite this article as:
Deng Xiao-Yue, Wang Hu, Wang Tao, Fang Xian-Tao, Zou Li-Li, Li Zhi-Ying and Liu Chang-Bai, Non-Viral Methods For Generating Integration-Free, Induced Pluripotent Stem Cells, Current Stem Cell Research & Therapy 2015; 10 (2) . https://dx.doi.org/10.2174/1574888X09666140923101914
DOI https://dx.doi.org/10.2174/1574888X09666140923101914 |
Print ISSN 1574-888X |
Publisher Name Bentham Science Publisher |
Online ISSN 2212-3946 |
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