Abstract
Although BSA Au clusters fluoresce in red region (λmax: 650nm), they are of limited use due to low fluorescence quantum yield (~6%). Here we report an enhanced fluorescence imaging application of fluorescent bio-nano probe BSA Au clusters using multipulse excitation scheme. Multipulse excitation takes advantage of long fluorescence lifetime (> 1µs) of BSA Au clusters and enhances its fluorescence intensity 15 times over short lived cellular auto-fluorescence. Moreover we have also shown that by using time gated detection strategy signal (fluorescence of BSA Au clusters) to noise (auto-fluorescence) ratio can be increased by 30 fold. Thereby with multipulse excitation long lifetime probes can be used to develop biochemical assays and perform optical imaging with zero background.
Keywords: Autofluorescence, BSA Au clusters, long lifetime, multipulse excitation, signal to noise.
Current Pharmaceutical Biotechnology
Title:BSA Au Clusters as a Probe for Enhanced Fluorescence Detection Using Multipulse Excitation Scheme
Volume: 14 Issue: 13
Author(s): Sangram L. Raut, Ryan Rich, Rafal Fudala, R. Kokate, J.D. Kimball, Julian Borejdo, Jamboor K. Vishwanatha, Zygmunt Gryczynski and Ignacy Gryczynski
Affiliation:
Keywords: Autofluorescence, BSA Au clusters, long lifetime, multipulse excitation, signal to noise.
Abstract: Although BSA Au clusters fluoresce in red region (λmax: 650nm), they are of limited use due to low fluorescence quantum yield (~6%). Here we report an enhanced fluorescence imaging application of fluorescent bio-nano probe BSA Au clusters using multipulse excitation scheme. Multipulse excitation takes advantage of long fluorescence lifetime (> 1µs) of BSA Au clusters and enhances its fluorescence intensity 15 times over short lived cellular auto-fluorescence. Moreover we have also shown that by using time gated detection strategy signal (fluorescence of BSA Au clusters) to noise (auto-fluorescence) ratio can be increased by 30 fold. Thereby with multipulse excitation long lifetime probes can be used to develop biochemical assays and perform optical imaging with zero background.
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Raut L. Sangram, Rich Ryan, Fudala Rafal, Kokate R., Kimball J.D., Borejdo Julian, Vishwanatha K. Jamboor, Gryczynski Zygmunt and Gryczynski Ignacy, BSA Au Clusters as a Probe for Enhanced Fluorescence Detection Using Multipulse Excitation Scheme, Current Pharmaceutical Biotechnology 2013; 14 (13) . https://dx.doi.org/10.2174/1389201015666140523161038
DOI https://dx.doi.org/10.2174/1389201015666140523161038 |
Print ISSN 1389-2010 |
Publisher Name Bentham Science Publisher |
Online ISSN 1873-4316 |
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