Abstract
Problem statement: Bauhinia tomentosa is a medicinal plant popularly used as herbal remedy for various ailments in Ayurveda. However, the scientific basis for its medicinal use especially in pain and inflammation remains unknown. Therefore, the present study was designed to evaluate analgesic and antioxidant potential of the ethanol, methanol and aqueous extract of Bauhinia tomentosa (stem).
Approach: The analgesic activity was determined for its central and peripheral pharmacological actions using Eddy’s hot plate method and acetic acid-induced writhing test in mice respectively. The ethanol, methanol and aqueous extracts were also investigated for its antioxidant potential through 1, 1- diphenyl-2-picrylhydrazyl (DPPH) radical scavenging and Nitric Oxide (NO) scavenging assays.
Results: Methanol and aqueous extract at the doses 200, 400 mg/kg and ethanol extract at high dose (400 mg/kg) significantly (p<0.05) inhibited number of writhing as well as increase in mean latency time. The ethanol extract at low dose (200 mg/kg) showed centrally anti-nociceptive effect but not peripherally. In acetic acid-induced writhing test, the ethanol, aqueous and methanol extract at the dose of 400 mg/kg produced a maximum of 81.21%, 79.18% and 72.5 % inhibition whereas reference drug Indomethacin showed a maximum inhibition of 79.26 % respectively. A dose dependent scavenging of DPPH radical scavenging has been observed with IC50 values of the methanol, ethanol and aqueous extracts were found to be 65.50, 167.4 & 85.85 µg/ml whereas the IC50 value for the reference ascorbic acid was 52.34 µg/ml. Extracts were also found to possess good nitric oxide scavenging potential with IC50 value of 220.43, 150.45 and 310.25 µg/ml of ethanol, methanol and aqueous extracts while IC50 value of standard ascorbic acid was found to be the 125.10 µg/ml respectively.
Keywords: Anti-nociceptive, acetic acid induced writhings, Bauhinia tomentosa, DPPH, Eddy’s hot plate method, nitric oxide scavenging potential.
Call for Papers in Thematic Issues
Ayurvedic medicinal therapeutic approaches for inflammatory and neurodegenerative disorders
Usually, in our elderly population, neurodegenerative disorders occur due to neuron death. Alzheimer?s disease (AD) is an irreversible and progressive neurodegenerative disease. Dementia is the leading cause of AD, which affects about 30 million people worldwide. According to the World Alzheimer report 2018, a new case of dementia develops every ...read more
Recent Advances in Biotransformation of Bioactive Terpenoids using Aspergillus niger
Biotransformation is the reaction catalyzed by biological catalysts of either isolated enzymes or whole cell cultures of microorganisms. This process has been extensively applied in the conversion of bioactive terpenoids with high regio- and stereospecifity. This process has been also applied in the lead discovery and development. Aspergillus niger has ...read more
Related Journals
Anti-Cancer Agents in Medicinal Chemistry
Current Analytical Chemistry
Current Computer-Aided Drug Design
Current Bioactive Compounds
Combinatorial Chemistry & High Throughput Screening
Current Organic Chemistry
Current Medicinal Chemistry
Current Organic Synthesis
Central Nervous System Agents in Medicinal Chemistry
Mini-Reviews in Organic Chemistry
Related Books
Botanicals and Natural Bioactives: Prevention and Treatment of Diseases
Biosurfactants: A Boon to Healthcare, Agriculture & Environmental Sustainability
Frontiers in Computational Chemistry
Advances in Dye Degradation
Biological and Medical Significance of Chemical Elements
Frontiers In Medicinal Chemistry
Advances in Organic Synthesis
Frontiers in Natural Product Chemistry
Recent Advances in Analytical Techniques
Advanced Catalysts Based on Metal-organic Frameworks (Part 2)
20