Abstract
A protective effect of α -ketobutyrate on hippocampal neuronal cells treated with H2O2 was tested. α - Ketobutyrate concentrations were as follows: 0.5 mM, 1 mM and 5 mM. Tests were performed at the temperature of 37 ºC. Moreover, the protective effect of α-ketobutyrate on the cytotoxicity of 100 and 40 nmol/4·103 cells treated by hydrogen peroxide was observed. Furthermore, the role of α-ketobutyrate as an energy substrate was also proven using the cis- [Cr(C2O4)(pm)(OH2)2]+ complex as a biosensor for the carbon dioxide uptake process. All the experiments were carried out under in-vitro conditions.
Keywords: Neuronal cells, molecular biosensor of CO2, chromium(III) complex.
Current Pharmaceutical Analysis
Title:Protective Effect of α-ketobutyrate on Survival of Hippocampal Neurons Challenged with Hydrogen Peroxide Chemistry Mimicking Brain Ischemia
Volume: 10 Issue: 2
Author(s): Dagmara Jacewicz, Kamila Siedlecka-Kroplewska, Joanna Pranczk, Urszula Popowska, Alicja Kuban-Jankowska, Narcyz Knap, Dariusz Wyrzykowski, Krzysztof Zamojc, Michal Wozniak and Lech Chmurzyrski
Affiliation:
Keywords: Neuronal cells, molecular biosensor of CO2, chromium(III) complex.
Abstract: A protective effect of α -ketobutyrate on hippocampal neuronal cells treated with H2O2 was tested. α - Ketobutyrate concentrations were as follows: 0.5 mM, 1 mM and 5 mM. Tests were performed at the temperature of 37 ºC. Moreover, the protective effect of α-ketobutyrate on the cytotoxicity of 100 and 40 nmol/4·103 cells treated by hydrogen peroxide was observed. Furthermore, the role of α-ketobutyrate as an energy substrate was also proven using the cis- [Cr(C2O4)(pm)(OH2)2]+ complex as a biosensor for the carbon dioxide uptake process. All the experiments were carried out under in-vitro conditions.
Export Options
About this article
Cite this article as:
Jacewicz Dagmara, Siedlecka-Kroplewska Kamila, Pranczk Joanna, Popowska Urszula, Kuban-Jankowska Alicja, Knap Narcyz, Wyrzykowski Dariusz, Zamojc Krzysztof, Wozniak Michal and Chmurzyrski Lech, Protective Effect of α-ketobutyrate on Survival of Hippocampal Neurons Challenged with Hydrogen Peroxide Chemistry Mimicking Brain Ischemia, Current Pharmaceutical Analysis 2014; 10 (2) . https://dx.doi.org/10.2174/1573412909666131231231631
DOI https://dx.doi.org/10.2174/1573412909666131231231631 |
Print ISSN 1573-4129 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-676X |
- Author Guidelines
- Graphical Abstracts
- Fabricating and Stating False Information
- Research Misconduct
- Post Publication Discussions and Corrections
- Publishing Ethics and Rectitude
- Increase Visibility of Your Article
- Archiving Policies
- Peer Review Workflow
- Order Your Article Before Print
- Promote Your Article
- Manuscript Transfer Facility
- Editorial Policies
- Allegations from Whistleblowers
- Announcements
Related Articles
-
Vitamin D Analogs as Modulators of Vitamin D Receptor Action
Current Topics in Medicinal Chemistry Platinum Compounds: A Hope for Future Cancer Chemotherapy
Anti-Cancer Agents in Medicinal Chemistry Structure-Based Virtual Screening for the Identification of High Affinity Compounds as Potent VEGFR2 Inhibitors for the Treatment of Renal Cell Carcinoma
Current Topics in Medicinal Chemistry LncRNA HOTAIR Polymorphisms Association with Cancer Susceptibility in Different Tumor Types
Current Drug Targets Clinical Pharmacogenetics of Methotrexate
Current Drug Metabolism Peroxisome Proliferator-Activated Receptor-γ: Master Regulator of Adipogenesis and Obesity
Current Stem Cell Research & Therapy Doxorubicin vs. ladirubicin: methods for improving osteosarcoma treatment
Mini-Reviews in Medicinal Chemistry Review of Noscapine and its Analogues as Potential Anti-Cancer Drugs
Mini-Reviews in Organic Chemistry Insulin-Like Growth Factor-I Molecular Pathways in Osteoblasts: Potential Targets for Pharmacological Manipulation
Current Molecular Pharmacology Triggering PIK3CA Mutations in PI3K/Akt/mTOR Axis: Exploration of Newer Inhibitors and Rational Preventive Strategies
Current Pharmaceutical Design A Comprehensive Review of the Genus Pyrola Herbs in Traditional Uses, Phytochemistry and Pharmacological Activities
Current Topics in Medicinal Chemistry Recent Progress on Apoptotic Activity of Triazoles
Current Drug Targets In <i>Vitro</i> Cytotoxicity Evaluation of Oxytetracycline Loaded Cockle Shell Derived Calcium Carbonate Aragonite Nanoparticles
Nanoscience & Nanotechnology-Asia A Critical Review on the Unexplored Therapeutic Treasure of Himalayan Ayurvedic Drug Daruharidra (<i>Berberis Aristata</i>)
Current Traditional Medicine A Novel Method for Screening of Anti-Cancer Drugs: Availability of Screening in Acidic Medium
Recent Patents on Biomedical Engineering (Discontinued) Radiolabelled Peptides: Eon in Radiopharmaceutical
Current Radiopharmaceuticals Therapeutic Applications of Crocus sativus L. (Saffron): A Review
The Natural Products Journal Recent Results on A-Ring Modification of 1α,25-Dihydroxyvitamin D3: Design and Synthesis of VDR-Agonists and Antagonists with High Biological Activity
Current Topics in Medicinal Chemistry The Mechanisms of Anticancer Agents by Genistein and Synthetic Derivatives of Isoflavone
Mini-Reviews in Medicinal Chemistry Chemosensitization by Antisense Oligonucleotides Targeting MDM2
Current Cancer Drug Targets