Purification of The Lipase B of Candida antarctica from a Commercial Enzymatic Extract

Title:Purification of The Lipase B of Candida antarctica from a Commercial Enzymatic Extract

Volume: 3 Issue: 2

Author(s): Carlos R. Llerena-Suster, Laura E. Briand and Susana R. Morcelle

Affiliation:

Keywords: Protein purification, CALB, UV absorbance, Bradford, size exclusion chromatography, Ion exchange chromatography.

Abstract: This paper presents a rational strategy to purify the lipase B of Candida antarctica of the commercial extract Lipozyme^® through size exclusion coupled with anionic exchange chromatography using a non conventional, easy to remove buffer system such as ammonia-ammonium. In this context, each step of the purification was followed through the determination of the protein content, esterase activity measurements, SDS-PAGE, agarose electrophoresis, UVspectroscopy and isoelectric focusing. The purification of the commercial extract afforded a sample that retains 47% of the proteins (being CALB the major enzymatic component of the purified sample) with a hydrolytic activity higher than the starting crude extract.

Cite this article as:

Llerena-Suster R. Carlos, Briand E. Laura and Morcelle R. Susana, Purification of The Lipase B of Candida antarctica from a Commercial Enzymatic Extract, Current Catalysis 2014; 3 (2) . https://dx.doi.org/10.2174/2211544702666131224234223