Abstract
An accurate, simple and sensitive method for determination of tiopronin (TP, N-(2-mercaptopropionyl)- glycine, Thiola) in human urine has been devised. Tiopronin is an important compound and is mainly used as a drug to prevent kidney stones. It works by removing the extra cystine from the body. Therefore, the reliable assay of tiopronin in biological matrices and pharmaceutical preparations is highly desirable. The proposed analytical procedure consists of reduction of tiopronin dimmer and its unsymmetrical disulfides with endogenous thiols, derivatization with 2-chloro-1- methylquinolinium tetrafluoroborate (CMQT) followed by acetonitrile stacking in capillary electrophoresis separation and ultraviolet-absorbance detection of TP-CMQT derivative at 350 nm. Baseline electrophoretic separation was achieved using standard fused-silica capillary (effective length 47.5 cm, 50 µm i.d.) and 0.25 mol L-1, Tris/HCl buffer adjusted to pH 2.0. The lower limit of quantitation for derivatized TP in urine was 5 µmol/L. The calibration curve showed linearity in the range 5-160 µmol L-1 urine with a regression coefficient corresponding to 0.9987 and the relative standard deviation of the points of the calibration curve being lower than 7%. The method can be used for a pharmacokinetic study with human subjects.
Keywords: Acetonitrile stacking, capillary electrophoresis, derivatization, tiopronin, ultraviolet detection, urine.
Current Analytical Chemistry
Title:Capillary Electrophoresis Determination of Tiopronin in Human Urine After Derivatization with 2-chloro-1-methylquinolinium Tetrafluoroborate
Volume: 10 Issue: 3
Author(s): Pawel Kubalczyk, Grazyna Chwatko and Edward Bald
Affiliation:
Keywords: Acetonitrile stacking, capillary electrophoresis, derivatization, tiopronin, ultraviolet detection, urine.
Abstract: An accurate, simple and sensitive method for determination of tiopronin (TP, N-(2-mercaptopropionyl)- glycine, Thiola) in human urine has been devised. Tiopronin is an important compound and is mainly used as a drug to prevent kidney stones. It works by removing the extra cystine from the body. Therefore, the reliable assay of tiopronin in biological matrices and pharmaceutical preparations is highly desirable. The proposed analytical procedure consists of reduction of tiopronin dimmer and its unsymmetrical disulfides with endogenous thiols, derivatization with 2-chloro-1- methylquinolinium tetrafluoroborate (CMQT) followed by acetonitrile stacking in capillary electrophoresis separation and ultraviolet-absorbance detection of TP-CMQT derivative at 350 nm. Baseline electrophoretic separation was achieved using standard fused-silica capillary (effective length 47.5 cm, 50 µm i.d.) and 0.25 mol L-1, Tris/HCl buffer adjusted to pH 2.0. The lower limit of quantitation for derivatized TP in urine was 5 µmol/L. The calibration curve showed linearity in the range 5-160 µmol L-1 urine with a regression coefficient corresponding to 0.9987 and the relative standard deviation of the points of the calibration curve being lower than 7%. The method can be used for a pharmacokinetic study with human subjects.
Export Options
About this article
Cite this article as:
Kubalczyk Pawel, Chwatko Grazyna and Bald Edward, Capillary Electrophoresis Determination of Tiopronin in Human Urine After Derivatization with 2-chloro-1-methylquinolinium Tetrafluoroborate, Current Analytical Chemistry 2014; 10 (3) . https://dx.doi.org/10.2174/1573411010999131219101552
DOI https://dx.doi.org/10.2174/1573411010999131219101552 |
Print ISSN 1573-4110 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-6727 |
Call for Papers in Thematic Issues
Advancements in biomedicine, corrosion inhibition/monitoring, and lubrication of carbon dots
Carbon dots, a class of carbon-based nanomaterials, have garnered substantial interest in various scientific and engineering fields due to their unique properties, including excellent optical properties, outstanding biocompatibility, easy preparation, good dispersity, ultrasmall size, low toxicity and environmental friendliness. This special issue of Current Analytical Chemistry aims to showcase cutting-edge ...read more
Advances in Microfluidic and Sensing Technologies of Biofluids.
Detection of biological fluids is an important means to assess human health and diagnose diseases. Currently, research on blood is relatively mature and widely applied in the medical field. With the development and advancement of microfluidic technology and novel detection methods, attention has turned to human physiological fluids such as ...read more
Analytical methods for environmental and food analysis
In recent years, the study of the chemical composition of food and the environment depends on analytical methods in order to acquire information about the chemical composition, processing, quality control and contamination of soil, water, air, plants and foods, aspects very important for food safety and environmental quality control. There ...read more
Applications of artificial intelligence in chemical analysis
Artificial intelligence (AI) has demonstrated its versatility in almost all scientific fields, especially analytical chemistry, which is leading the way in the integration of AI. According to Chemical Abstract Services, one of the major disciplines contributing to AI-related publications and patents is analytical chemistry. That?s because techniques such as deep ...read more
- Author Guidelines
- Graphical Abstracts
- Fabricating and Stating False Information
- Research Misconduct
- Post Publication Discussions and Corrections
- Publishing Ethics and Rectitude
- Increase Visibility of Your Article
- Archiving Policies
- Peer Review Workflow
- Order Your Article Before Print
- Promote Your Article
- Manuscript Transfer Facility
- Editorial Policies
- Allegations from Whistleblowers
Related Articles
-
Meet Our Regional Editor:
Medicinal Chemistry Recent Developments in Female Hormonal Contraception
Current Women`s Health Reviews Stem Cell Therapy: A Promising Approach in Treatment of COVID-19
Current Stem Cell Research & Therapy Regulatory T Cells as Targets for Immunotherapy of Autoimmunity and Inflammation
Inflammation & Allergy - Drug Targets (Discontinued) Neuropathic Pain: Is the End of Suffering Starting in the Gene Therapy?
Current Drug Targets Mast Cells and Basophils: Trojan Horses of Conventional Lin- Stem/Progenitor Cell Isolates
Current Pharmaceutical Design Insights on the Neuromodulatory Propensity of Selaginella (Sanjeevani) and its Potential Pharmacological Applications
CNS & Neurological Disorders - Drug Targets Immune Cell Entry to Central Nervous System - Current Understanding and Prospective Therapeutic Targets
Endocrine, Metabolic & Immune Disorders - Drug Targets A Targeted Therapy for Protein and Lipid Kinases in Chronic Lymphocytic Leukemia
Current Medicinal Chemistry Contribution of Glucocorticoids and Glucocorticoid Receptors to the Regulation of Neurodegenerative Processes
CNS & Neurological Disorders - Drug Targets Extracts of Bacopa monnieri (L) Pennell Down-Regulate the Expression of Leukotriene C<sub>4</sub> Synthase mRNA in HL-60 Cells and Suppress OVAInduced Inflammation in BALB/c Mice
Current Bioactive Compounds Mechanistic Systems Biology of Inflammatory Gene Expression in Airway Smooth Muscle as Tool for Asthma Drug Development
Current Drug Discovery Technologies Role of Nitric Oxide and Reactive Oxygen Species in Arthritis
Current Pharmaceutical Design Role of Monitoring Thiopurine Methyltransferase (TPMT) Activity in the Individualized Therapy with Azathioprine or 6-Mercaptopurine
Current Pharmacogenomics and Personalized Medicine Antioxidant Food Supplements and Obesity-Related Inflammation
Current Medicinal Chemistry 4Aβ1-15-Derived Monoclonal Antibody Reduces More Aβ Burdens and Neuroinflammation than Homologous Vaccine in APP/PS1 Mice
Current Alzheimer Research Anti-Inflammatory Therapy for Alzheimer’s Disease from Epidemiological Fact to New Mechanisms of Action
Anti-Inflammatory & Anti-Allergy Agents in Medicinal Chemistry Apolipoprotein E Gene Variants of Alzheimer's Disease and Vascular Dementia Patients in a Community Population of Nanking
Medicinal Chemistry Hitting the Golden TORget: Curcumin’s Effects on mTOR Signaling
Anti-Cancer Agents in Medicinal Chemistry Identifying the Hub Genes and Immune Cell Infiltration in Synovial Tissue between Osteoarthritic and Rheumatoid Arthritic Patients by Bioinformatic Approach
Current Pharmaceutical Design