Abstract
Mycobacterium tuberculosis is the causative agent of tuberculosis, an old formidable scourge of global public health. Despite decades of intensive studies on the biology and physiology of M.tuberculosis, the function of about one-quarter open reading frame (ORF) of the M.tuberculosis genome remains elusive. In order to determine the size of M.tuberculosis proteome during normal in vitro growth, shotgun proteomic profiling and cross-reference to available multi-omics data were performed. We identified 2817 M.tuberculosis ORFs, about 70% of the predicted encoding capacity. More interestingly, 228 novel proteins which had not been predicted by the 1998 genomic annotation were found. These novel proteins participated in various cellular events including intracellular growth, persistence and dormancy. The proteins with potential value in diagnostic biomarker, drug target and host immune response targets were thoroughly aligned with available transcriptional data. One unexpected discovery is the absence of the key components involved in the base-excision repair pathway which was crucial for M.tuberculosis survival. These data provided fresh insights into the biology of this old pathogen.
Keywords: Proteome, Physiology, Multi-omics, Pathogenesis, Mycobacterium tuberculosis, Tuberculosis.
Current Proteomics
Title:Mycobacterium tuberculosis Biology Revealed by Proteome Profiling and Integration of Multi-omics Data—Proteomics Insight into M. tuberculosis Systems Biology
Volume: 10 Issue: 3
Author(s): Wanyan Deng, Xiangyu Fan, Xiaozhen Wang, Ying He, Zihui Li, Jie Song, Lingyan Ping, Lei Chang, Duc Minh Duong, Zongde Zhang, Ping Xu and Jianping Xie
Affiliation:
Keywords: Proteome, Physiology, Multi-omics, Pathogenesis, Mycobacterium tuberculosis, Tuberculosis.
Abstract: Mycobacterium tuberculosis is the causative agent of tuberculosis, an old formidable scourge of global public health. Despite decades of intensive studies on the biology and physiology of M.tuberculosis, the function of about one-quarter open reading frame (ORF) of the M.tuberculosis genome remains elusive. In order to determine the size of M.tuberculosis proteome during normal in vitro growth, shotgun proteomic profiling and cross-reference to available multi-omics data were performed. We identified 2817 M.tuberculosis ORFs, about 70% of the predicted encoding capacity. More interestingly, 228 novel proteins which had not been predicted by the 1998 genomic annotation were found. These novel proteins participated in various cellular events including intracellular growth, persistence and dormancy. The proteins with potential value in diagnostic biomarker, drug target and host immune response targets were thoroughly aligned with available transcriptional data. One unexpected discovery is the absence of the key components involved in the base-excision repair pathway which was crucial for M.tuberculosis survival. These data provided fresh insights into the biology of this old pathogen.
Export Options
About this article
Cite this article as:
Deng Wanyan, Fan Xiangyu, Wang Xiaozhen, He Ying, Li Zihui, Song Jie, Ping Lingyan, Chang Lei, Minh Duong Duc, Zhang Zongde, Xu Ping and Xie Jianping, Mycobacterium tuberculosis Biology Revealed by Proteome Profiling and Integration of Multi-omics Data—Proteomics Insight into M. tuberculosis Systems Biology, Current Proteomics 2013; 10 (3) . https://dx.doi.org/10.2174/15701646113106660002
DOI https://dx.doi.org/10.2174/15701646113106660002 |
Print ISSN 1570-1646 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-6247 |
Call for Papers in Thematic Issues
Mass spectrometry data acquisition and analysis for proteomics
The Thematic Issue on "Mass spectrometry data acquisition and analysis for proteomics" aims to explore the latest advancements and challenges in the field of proteomics through the lens of mass spectrometry. Proteomics, the large-scale study of proteins and their functions, plays a crucial role in understanding various biological processes and ...read more
Peptides: State-of-Art and Commercialisation Hurdles
The Editors of the Current Proteomics (CP) journal are highly privileged to welcome scientists to submit their scientific research and review articles to be considered for publication in the upcoming thematic issue. The topics should cover various aspects of peptides in regard to their synthetic methodologies, formulation approaches, pharmacological challenges, ...read more
- Author Guidelines
- Graphical Abstracts
- Fabricating and Stating False Information
- Research Misconduct
- Post Publication Discussions and Corrections
- Publishing Ethics and Rectitude
- Increase Visibility of Your Article
- Archiving Policies
- Peer Review Workflow
- Order Your Article Before Print
- Promote Your Article
- Manuscript Transfer Facility
- Editorial Policies
- Allegations from Whistleblowers
- Announcements
Related Articles
-
Current Trends and Future Approaches in Small-Molecule Therapeutics for COVID-19
Current Medicinal Chemistry Recent Developments in Phytosterol Recovery from Oil Deodorizer Distillates
Current Nutrition & Food Science Bioactive Metabolites from Pathogenic and Endophytic Fungi of Forest Trees
Current Medicinal Chemistry Pyrroles and Fused Pyrroles: Synthesis and Therapeutic Activities
Mini-Reviews in Organic Chemistry Model Systems for Pulmonary Infectious Diseases: Paradigms of Anthrax and Tuberculosis
Current Topics in Medicinal Chemistry Codon Usage Biases in Alzheimers Disease and Other Neurodegenerative Diseases
Protein & Peptide Letters Protein-protein Interfaces Integrated into Interaction Networks: Implications on Drug Design
Current Pharmaceutical Design Prevalence of Toxoplasma Encephalitis in AIDS Patients Treated with Didanosine Hospitalised in a French Infectious Service
Current HIV Research Sulfated Oligosaccharides: New Targets for Drug Development?
Current Medicinal Chemistry Anti-pathogenic Strategies for Fighting Pseudomonas aeruginosa Infections- probiotic Soluble Compounds as Inhibitors of Quorum Sensing Genes Expression
Current Organic Chemistry Applications of the Combination of Microwave and Parallel Synthesis in Medicinal Chemistry
Combinatorial Chemistry & High Throughput Screening Lectin Techniques for Glycoproteomics
Current Proteomics Antioxidant and Antimicrobial Activities of Cinnamic Acid Derivatives
Mini-Reviews in Medicinal Chemistry Large Disclosing the Nature of Computational Tools for the Analysis of Next Generation Sequencing Data
Current Topics in Medicinal Chemistry Novel Approach to the Role of NMDA Receptors in Traumatic Brain Injury
CNS & Neurological Disorders - Drug Targets Review of Structures Containing Fullerene-C60 for Delivery of Antibacterial Agents. Multitasking model for Computational Assessment of Safety Profiles
Current Bioinformatics Subject Index To Volume 13
Current Medicinal Chemistry The Diagnostic Utility of Pleural Fluid Tests in Clinical Practice
Current Respiratory Medicine Reviews Exploring Coumarin and Chalcone Analogues as Potential Antimycobacterial Agents
Anti-Infective Agents iATP: A Sequence Based Method for Identifying Anti-tubercular Peptides
Medicinal Chemistry