Abstract
The aim of this study is optimization of experimental factors in a UV-visible spectrophotometric method for simultaneous determination of dorzolamide hydrochloride and timolol maleate in eye drops. For this purpose, partial least squares-1 method was developed for resolving overlapped UV-visible spectra of these compounds. Principal component number, wavelength range (number of wavelengths) and data preprocessing applications were investigated as experimental factors. The affected experimental response in this method is PRESS (Prediction Error Sum of Squares) values. Response Surface Methodology (RSM) method was used for interpretation of relationships between these factors and PRESS values, and results were shown as equations and contour graphs. Multivariate calibration techniques which were used in the method development have some advantages such as using whole spectrum for the simultaneous analysis of the mixture component that allow to minimize the effects of noise peak, shorter analysis time, low cost and simple instrumentation without any chemical pre-treatment. This optimized method was validated and applied to a pharmaceutical preparation, eye drops, and results were compared with a literature method statistically and no significant difference was observed.
Keywords: Multivariate optimization, PLS-1, validation, pharmaceutical preparation, dorzolamide hydrochloride, timolol maleate, eye drops.
Current Pharmaceutical Analysis
Title:Multivariate Optimization Model in a Partial Least Squares-1 Method for Simultaneous Determination of Dorzolamide Hydrochloride and Timolol Maleate in Eye Drops
Volume: 9 Issue: 4
Author(s): Ismail Murat Palabiyik, Ece Goker, Mehmet Gokhan Caglayan and Feyyaz Onur
Affiliation:
Keywords: Multivariate optimization, PLS-1, validation, pharmaceutical preparation, dorzolamide hydrochloride, timolol maleate, eye drops.
Abstract: The aim of this study is optimization of experimental factors in a UV-visible spectrophotometric method for simultaneous determination of dorzolamide hydrochloride and timolol maleate in eye drops. For this purpose, partial least squares-1 method was developed for resolving overlapped UV-visible spectra of these compounds. Principal component number, wavelength range (number of wavelengths) and data preprocessing applications were investigated as experimental factors. The affected experimental response in this method is PRESS (Prediction Error Sum of Squares) values. Response Surface Methodology (RSM) method was used for interpretation of relationships between these factors and PRESS values, and results were shown as equations and contour graphs. Multivariate calibration techniques which were used in the method development have some advantages such as using whole spectrum for the simultaneous analysis of the mixture component that allow to minimize the effects of noise peak, shorter analysis time, low cost and simple instrumentation without any chemical pre-treatment. This optimized method was validated and applied to a pharmaceutical preparation, eye drops, and results were compared with a literature method statistically and no significant difference was observed.
Export Options
About this article
Cite this article as:
Palabiyik Murat Ismail, Goker Ece, Caglayan Gokhan Mehmet and Onur Feyyaz, Multivariate Optimization Model in a Partial Least Squares-1 Method for Simultaneous Determination of Dorzolamide Hydrochloride and Timolol Maleate in Eye Drops, Current Pharmaceutical Analysis 2013; 9 (4) . https://dx.doi.org/10.2174/1573412911309040008
DOI https://dx.doi.org/10.2174/1573412911309040008 |
Print ISSN 1573-4129 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-676X |
- Author Guidelines
- Graphical Abstracts
- Fabricating and Stating False Information
- Research Misconduct
- Post Publication Discussions and Corrections
- Publishing Ethics and Rectitude
- Increase Visibility of Your Article
- Archiving Policies
- Peer Review Workflow
- Order Your Article Before Print
- Promote Your Article
- Manuscript Transfer Facility
- Editorial Policies
- Allegations from Whistleblowers
- Announcements
Related Articles
-
Polyphenols Regulate Endothelial Functions and Reduce the Risk of Cardiovascular Disease
Current Pharmaceutical Design Editorial [Hot Topic:Platelets in Inflammation and Inflammatory Disorders Guest Editor: Armen Yuri Gasparyan ]
Inflammation & Allergy - Drug Targets (Discontinued) Hypertension - Current Natural Strategies to Lower Blood Pressure
Current Pharmaceutical Design Uric Acid Metabolism in Pre-hypertension and the Metabolic Syndrome
Current Vascular Pharmacology L-4F Differentially Alters Plasma Levels of Oxidized Fatty Acids Resulting in more Anti-Inflammatory HDL in Mice
Drug Metabolism Letters Associations between Adiponectin Gene Variability, Proinflammatory and Angiogenetic Markers: Implications for Microvascular Disease Development in Type 2 Diabetes Mellitus?
Current Vascular Pharmacology Sex Differences in Clinical Outcomes of Patients with Stable Coronary Artery Disease after Percutaneous Coronary Intervention
Current Pharmaceutical Design Natural Polyphenols and Cardioprotection
Mini-Reviews in Medicinal Chemistry The Role of P-glycoprotein in Cerebral Amyloid Angiopathy; Implications for the Early Pathogenesis of Alzheimers Disease
Current Alzheimer Research Meet Our Regional Editor:
Current Organic Chemistry Effective Inhibition of Foam Cells Formation by Tanshinone IIA in RAW264.7 Macrophages Induced with LDL Isolated from Hypercholesterolemia Patients: A Proteomic Analysis
Current Proteomics Effect of Colestimide on Reduction of Body Weight and Waist Circumference in Metabolic Syndrome Patients with Cardiovascular Risk Factors
Vascular Disease Prevention (Discontinued) Xanthine Oxidase Inhibitor Febuxostat as a Novel Agent Postulated to Act Against Vascular Inflammation
Anti-Inflammatory & Anti-Allergy Agents in Medicinal Chemistry EDITORIAL [Pharmacological Treatments for Obesity and Obesity-Hypertension (Guest Editor: Kazuko Masuo)]
Immunology, Endocrine & Metabolic Agents in Medicinal Chemistry (Discontinued) Large A-V Fistula: Pathophysiological Consequences and Therapeutic Perspectives
Current Vascular Pharmacology A Systematic Review of Drugs in Late-Stage Development for the Treatment of Multiple Sclerosis: A Focus on Oral Synthetic Drugs
Inflammation & Allergy - Drug Targets (Discontinued) Leptin as a Cardiac Pro-Hypertrophic Factor and its Potential Role in the Development of Heart Failure
Current Pharmaceutical Design Potential Role of Polyphenols in the Prevention of Cardiovascular Diseases: Molecular Bases
Current Medicinal Chemistry Subject Index to Volume 5
Current Drug Metabolism Erratum
Inflammation & Allergy - Drug Targets (Discontinued)