Abstract
The present study deals with the green synthesis of silver nanoparticles (AgNPs) using Citrus reticulata juice as the reducing agent. The reaction carried out at room temperature (25°C) and at 55°C. The AgNPs were characterized by UV-Vis, TEM, XRD, AFM and FTIR measurements. The produced AgNPs were also evaluated for their increased antibacterial activities with tetracycline and penicillin against Gram-positive and Gram-negative bacteria. In this study, combination of AgNPs (at 55°C) and penicillin showed high inhibition zone in E. coli (18 mm) compared to that of AgNPs (at 25°C) and tetracycline. The cytotoxic effects of AgNPs from the Citrus reticulate juice on B16/ F10 melanoma cell line were evaluated by MTT (3-(4, 5-Dimethylthiazol-2-yl)-2, 5-Diphenyltetrazolium Bromide) assay. MTT assay showed a minimum cell inhibition (14.83%) at 0.125 mM (AgNPs at 25°C) and a maximum cell inhibition (31.29%) was observed at 1 mM (AgNPs at 55°C).
Keywords: Green synthesis, Citrus reticulata juice, AgNPs, antibacterial studies, cytotoxicity.
Current Nanoscience
Title:Green Synthesis of Silver Nanoparticles Using Citrus Reticulata Juice and Evaluation of their Antibacterial Activity and Cytotoxicity Against Melanoma-B16/F10 Cells
Volume: 9 Issue: 4
Author(s): T.V.M. Sreekanth, P. C. Nagajyothi, T.N.V.K.V. Prasad and Kap Duk Lee
Affiliation:
Keywords: Green synthesis, Citrus reticulata juice, AgNPs, antibacterial studies, cytotoxicity.
Abstract: The present study deals with the green synthesis of silver nanoparticles (AgNPs) using Citrus reticulata juice as the reducing agent. The reaction carried out at room temperature (25°C) and at 55°C. The AgNPs were characterized by UV-Vis, TEM, XRD, AFM and FTIR measurements. The produced AgNPs were also evaluated for their increased antibacterial activities with tetracycline and penicillin against Gram-positive and Gram-negative bacteria. In this study, combination of AgNPs (at 55°C) and penicillin showed high inhibition zone in E. coli (18 mm) compared to that of AgNPs (at 25°C) and tetracycline. The cytotoxic effects of AgNPs from the Citrus reticulate juice on B16/ F10 melanoma cell line were evaluated by MTT (3-(4, 5-Dimethylthiazol-2-yl)-2, 5-Diphenyltetrazolium Bromide) assay. MTT assay showed a minimum cell inhibition (14.83%) at 0.125 mM (AgNPs at 25°C) and a maximum cell inhibition (31.29%) was observed at 1 mM (AgNPs at 55°C).
Export Options
About this article
Cite this article as:
Sreekanth T.V.M., Nagajyothi C. P., Prasad T.N.V.K.V. and Lee Duk Kap, Green Synthesis of Silver Nanoparticles Using Citrus Reticulata Juice and Evaluation of their Antibacterial Activity and Cytotoxicity Against Melanoma-B16/F10 Cells, Current Nanoscience 2013; 9 (4) . https://dx.doi.org/10.2174/1573413711309040007
DOI https://dx.doi.org/10.2174/1573413711309040007 |
Print ISSN 1573-4137 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-6786 |
Call for Papers in Thematic Issues
Role of nanomaterials in fabrication of coatings, Machining and Joining
The application of nanoscience has brought about a revolution in the field of mechanical engineering by providing novel materials, boosting manufacturing processes, and generating cutting-edge products. The purpose of this special issue is to investigate the enormous impact that nanoscience has had on mechanical engineering, with a particular emphasis on ...read more
Advanced Inorganic Nanocomposites and Their Emerging Applications
This special issue collection will highlight developments on the recent trends about the synthesis of metal oxides, nanoclusters, biomaterials, 2D nanomaterials, nanocrystals, nanocomposites, etc. and their applications in electrochemical systems, tissue regeneration, energy storage and harvesting, sensors, etc. The novelty of the methods in the chemical synthesis and their characterizations, ...read more
Applicability of Nanotechnology for Performance Enhancement of Clean Energy Technologies
Population growth, industrialization, and improvement in living quality would lead to further increase in energy demand in near future. Regarding the disadvantages of fossil fuels such as fluctuations in their price, high emissions of greenhouse gases and restriction of their sources, it is crucial to use and exploit alternative energy ...read more
Graphene and 2D Materials for Energy Storage and Conversion
This thematic issue will discuss the recent advances in graphene-based nanomaterials for different energy technologies. Graphene possesses a high surface area, and stable structure and exhibits many interesting electronic, optical, and mechanical properties due to its 2D crystal structure. Graphene is of both fundamental interest and suitable for a wide ...read more
Related Journals
- Author Guidelines
- Graphical Abstracts
- Fabricating and Stating False Information
- Research Misconduct
- Post Publication Discussions and Corrections
- Publishing Ethics and Rectitude
- Increase Visibility of Your Article
- Archiving Policies
- Peer Review Workflow
- Order Your Article Before Print
- Promote Your Article
- Manuscript Transfer Facility
- Editorial Policies
- Allegations from Whistleblowers
Related Articles
-
Integrins in Drug Targeting-RGD Templates in Toxins
Current Pharmaceutical Design Emulsomes Meet S-layer Proteins: An Emerging Targeted Drug Delivery System
Current Pharmaceutical Biotechnology Tyrosine Kinase Blockers: New Hope for Successful Cancer Therapy
Anti-Cancer Agents in Medicinal Chemistry Advanced Management Options for Endometriosis
Current Women`s Health Reviews Inflammatory Cytokines, Growth Factors, and Depression
Current Pharmaceutical Design Computer Aided Drug Design Approaches for Identification of Novel Autotaxin (ATX) Inhibitors
Current Medicinal Chemistry Synthesis, Spectroscopic Properties, Crystal Structure And Biological Evaluation of New Platinum Complexes with 5-methyl-5-(2-thiomethyl)ethyl Hydantoin
Anti-Cancer Agents in Medicinal Chemistry Agonists of the Tissue-Protective Erythropoietin Receptor in the Treatment of Parkinson’s Disease
Current Topics in Medicinal Chemistry Thinking Outside the ‘Block’: Alternative Polymer Compositions for Micellar Drug Delivery
Current Topics in Medicinal Chemistry Current Landscape of Natural Products against Coronaviruses: Perspectives in COVID-19 Treatment and Anti-viral Mechanism
Current Pharmaceutical Design Advances in DNA-Ligands with Groove Binding, Intercalating and/or Alkylating Activity: Chemistry, DNA-Binding and Biology
Current Medicinal Chemistry Combating P-glycoprotein-Mediated Multidrug Resistance Using Therapeutic Nanoparticles
Current Pharmaceutical Design Nonviral Gene Therapy
Current Gene Therapy Peptide-Receptor Ligands and Multivalent Approach
Anti-Cancer Agents in Medicinal Chemistry Allosteric Modulators for Adenosine Receptors: An Alternative to the Orthosteric Ligands
Current Topics in Medicinal Chemistry Synthetic Lethality and PARP-Inhibitors in Oral and Head & Neck Cancer
Current Pharmaceutical Design Has Selenium a Chemopreventive Effect on Hepatocellular Carcinoma?
Mini-Reviews in Medicinal Chemistry Reactive Oxygen Species in Pathogenesis of Atherosclerosis
Current Pharmaceutical Design Cell Proliferation and Cytotoxicity Assays
Current Pharmaceutical Biotechnology Molecular Probes for Malignant Melanoma Imaging
Current Pharmaceutical Biotechnology