Abstract
The present study assessed the phenotypic aspects of oral-cavity Candida albicans isolates from 300 HIV-1- positive patients, relating the most commonly investigated virulence factors (enzyme typing and germ-tube formation) to the most common morphotypes. The samples were seeded into specific media for isolation and subsequent identification using the automated Vitek 2 system. The following assays were performed for phenotypic characterization: morphotyping, germ-tube formation and enzyme typing. Out of 300 collected samples, 144 tested positive for yeasts of the Candida genus, 98 (32.7 %) of which were identified as C. albicans. The latter samples were attributed to seven different morphotypes; the three most common morphotypes were 7208 (49 %), 7308 (14.3 %) and 3208 (13.3 %). All of the C. albicans isolate samples formed germ tubes and produced the enzymes proteinase and phospholipase, with an activity classified as intermediate to high. Due to the identification of virulence factors among the analyzed samples, monitoring of HIV-1-positive patients colonized by different morphotypes must be established because these morphotypes are extremely pathogenic and can trigger severe fungal infections.
Keywords: HIV-1, Co-infection, Candida albicans, oral cavity, virulence factors.
Current HIV Research
Title:Virulence Factors of Candida albicans Isolates from the Oral Cavities of HIV-1-Positive Patients
Volume: 11 Issue: 4
Author(s): Tatiany O.A. Menezes, Luciana C.S. Gillet, Sílvio A.F. Menezes, Rosimar N.M. Feitosa, Marluísa O.G. Ishak, Ricardo Ishak, Sílvia H. Marques-da-Silva and Antonio C.R. Vallinoto
Affiliation:
Keywords: HIV-1, Co-infection, Candida albicans, oral cavity, virulence factors.
Abstract: The present study assessed the phenotypic aspects of oral-cavity Candida albicans isolates from 300 HIV-1- positive patients, relating the most commonly investigated virulence factors (enzyme typing and germ-tube formation) to the most common morphotypes. The samples were seeded into specific media for isolation and subsequent identification using the automated Vitek 2 system. The following assays were performed for phenotypic characterization: morphotyping, germ-tube formation and enzyme typing. Out of 300 collected samples, 144 tested positive for yeasts of the Candida genus, 98 (32.7 %) of which were identified as C. albicans. The latter samples were attributed to seven different morphotypes; the three most common morphotypes were 7208 (49 %), 7308 (14.3 %) and 3208 (13.3 %). All of the C. albicans isolate samples formed germ tubes and produced the enzymes proteinase and phospholipase, with an activity classified as intermediate to high. Due to the identification of virulence factors among the analyzed samples, monitoring of HIV-1-positive patients colonized by different morphotypes must be established because these morphotypes are extremely pathogenic and can trigger severe fungal infections.
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Menezes O.A. Tatiany, Gillet C.S. Luciana, Menezes A.F. Sílvio, Feitosa N.M. Rosimar, Ishak O.G. Marluísa, Ishak Ricardo, Marques-da-Silva H. Sílvia and Vallinoto C.R. Antonio, Virulence Factors of Candida albicans Isolates from the Oral Cavities of HIV-1-Positive Patients, Current HIV Research 2013; 11 (4) . https://dx.doi.org/10.2174/1570162X113119990042
DOI https://dx.doi.org/10.2174/1570162X113119990042 |
Print ISSN 1570-162X |
Publisher Name Bentham Science Publisher |
Online ISSN 1873-4251 |
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