Abstract
This work developed an alternative approach targeting the evaluation of the aggregation propensity of the (1- 42) β-amyloid peptide (Alzheimer’s disease) and some segments, either attached to a polymer during their synthesis or when free in solution. The solvation behavior of peptide-resins was gauged by measuring the swelling of beads in a microscope and the degree of chain motion through EPR spectra of previously labeled resins with an amino acid-type probe. In terms of comparative solvent dissociation power towards aggregated structures, the findings revealed greater values of peptide-resin swelling, peptide chain mobility and solubility when in strong electron donor dimethylsulfoxide than in strong electron acceptor trifluoroethanol. Otherwise, the weakest chain-chain disruption power was verified for acetonitrile, an internally neutral solvent in terms of Lewis acid/base properties. In complement, fluorescence and light scattering experiments depicted that the 15-35 region plays an essential role in the amyloid peptide fibril formation capacity.
Keywords: β-amyloid peptide, electron spin resonance, fibril formation, peptide solubilization, polymer, polymer solvation.
Protein & Peptide Letters
Title:Assessment of the Aggregation Propensity of the β -amyloid Peptide During the Synthesis and when Free in Solution
Volume: 20 Issue: 8
Author(s): Luciana Malavolta, Marcelo R.S. Pinto and Clóvis R. Nakaie
Affiliation:
Keywords: β-amyloid peptide, electron spin resonance, fibril formation, peptide solubilization, polymer, polymer solvation.
Abstract: This work developed an alternative approach targeting the evaluation of the aggregation propensity of the (1- 42) β-amyloid peptide (Alzheimer’s disease) and some segments, either attached to a polymer during their synthesis or when free in solution. The solvation behavior of peptide-resins was gauged by measuring the swelling of beads in a microscope and the degree of chain motion through EPR spectra of previously labeled resins with an amino acid-type probe. In terms of comparative solvent dissociation power towards aggregated structures, the findings revealed greater values of peptide-resin swelling, peptide chain mobility and solubility when in strong electron donor dimethylsulfoxide than in strong electron acceptor trifluoroethanol. Otherwise, the weakest chain-chain disruption power was verified for acetonitrile, an internally neutral solvent in terms of Lewis acid/base properties. In complement, fluorescence and light scattering experiments depicted that the 15-35 region plays an essential role in the amyloid peptide fibril formation capacity.
Export Options
About this article
Cite this article as:
Malavolta Luciana, Pinto R.S. Marcelo and Nakaie R. Clóvis, Assessment of the Aggregation Propensity of the β -amyloid Peptide During the Synthesis and when Free in Solution, Protein & Peptide Letters 2013; 20 (8) . https://dx.doi.org/10.2174/0929866511320080002
DOI https://dx.doi.org/10.2174/0929866511320080002 |
Print ISSN 0929-8665 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-5305 |
- Author Guidelines
- Graphical Abstracts
- Fabricating and Stating False Information
- Research Misconduct
- Post Publication Discussions and Corrections
- Publishing Ethics and Rectitude
- Increase Visibility of Your Article
- Archiving Policies
- Peer Review Workflow
- Order Your Article Before Print
- Promote Your Article
- Manuscript Transfer Facility
- Editorial Policies
- Allegations from Whistleblowers
Related Articles
-
Astrocytes Exert and Control Immune Responses in the Brain
Current Immunology Reviews (Discontinued) The Effects of Vitamin B in Depression
Current Medicinal Chemistry Meet Our Editorial Board Member
CNS & Neurological Disorders - Drug Targets 3D-QSAR and Molecular Docking Studies of Flavonoid Derivatives as Potent Acetylcholinesterase Inhibitors
Letters in Drug Design & Discovery Blood-Brain Barrier P-Glycoprotein Function in Neurodegenerative Disease
Current Pharmaceutical Design HDL Genetic Defects
Current Pharmaceutical Design Cerebrolysin, a Mixture of Neurotrophic Factors Induces Marked Neuroprotection in Spinal Cord Injury Following Intoxication of Engineered Nanoparticles from Metals
CNS & Neurological Disorders - Drug Targets Sigma-1 Receptor Agonists as Therapeutic Drugs for Cognitive Impairment in Neuropsychiatric Diseases
Current Pharmaceutical Design GSK-3 Inhibitors: Recent Developments and Therapeutic Potential
Current Signal Transduction Therapy Combination Therapy of Inhaled Corticosteroids and Long-Acting β2- Adrenergics In Management of Patients with Chronic Obstructive Pulmonary Disease
Current Pharmaceutical Design Peripheral Benzodiazepine Receptor (PBR) New Insight in Cell Proliferation and Cell Differentiation Review
Current Clinical Pharmacology Vascular Injury During Elevated Glucose can be Mitigated by Erythropoietin and Wnt Signaling
Current Neurovascular Research The Role of Autophagy in the Pathogenesis of Ischemic Stroke
Current Neuropharmacology Subject Index to Volume 3
Current Medicinal Chemistry - Immunology, Endocrine & Metabolic Agents Secondary Prevention of Ischemic Stroke
Current Drug Targets Natural and Synthetic Inhibitors of Caspases: Targets for Novel Drugs
Current Drug Targets - CNS & Neurological Disorders Infection, its Treatment and the Risk for Stroke
Current Vascular Pharmacology Using Mass Spectrometry-Based Peptidomics to understand the Brain and Disorders such as Parkinson’s Disease and Schizophrenia
Current Topics in Medicinal Chemistry SPECT Imaging and Cerebrovascular Disease
Vascular Disease Prevention (Discontinued) Repurposed Drugs as Potential Therapeutic Candidates for the Management of Alzheimer's Disease
Current Drug Metabolism