Abstract
Curcumin ((1E,6E)-1,7-bis(4-hydroxy-3-methoxyphenyl) hepta-1,6-diene-3,5-dione) is a naturally occurring polyphenol. We have investigated effects of curcumin on human erythrocytes membrane naïve and subjected to in vitro oxidative stress by incubating with tert-butylhydroperoxide (t-BHP). Human erythrocytes have been chosen for the study model due to their being the primary target site for the oxidative stress generated by extracellular as well as intracellular metabolic pathway and substances. Acetylcholinesterase (AChE) in the erythrocytes membrane shows many properties similar to those of brain tissues AChE activity may be considered as central cholinergic status. Results show that curcumin (10-5 M to 10-8 M) significantly (p<0.001) protects AChE activity in erythrocyte during oxidative stress. AChE activity in naïve membrane was found to be maximum at 10-6 M of curcumin. Curcumin at 10-6 M caused decrease in Km of the enzyme Our results signify the potential protective effect of curcumin on the cholinergic system under conditions of oxidative stress.
Keywords: Acetylcholine esterase, Antioxidants, Curcumin, Malondialdehyde, Oxidative stress, Membrane fluidity.
Letters in Drug Design & Discovery
Title:Curcumin Activates Erythrocyte Membrane Acetylcholinesterase
Volume: 10 Issue: 6
Author(s): Prabhakar Singh and Syed Ibrahim Rizvi
Affiliation:
Keywords: Acetylcholine esterase, Antioxidants, Curcumin, Malondialdehyde, Oxidative stress, Membrane fluidity.
Abstract: Curcumin ((1E,6E)-1,7-bis(4-hydroxy-3-methoxyphenyl) hepta-1,6-diene-3,5-dione) is a naturally occurring polyphenol. We have investigated effects of curcumin on human erythrocytes membrane naïve and subjected to in vitro oxidative stress by incubating with tert-butylhydroperoxide (t-BHP). Human erythrocytes have been chosen for the study model due to their being the primary target site for the oxidative stress generated by extracellular as well as intracellular metabolic pathway and substances. Acetylcholinesterase (AChE) in the erythrocytes membrane shows many properties similar to those of brain tissues AChE activity may be considered as central cholinergic status. Results show that curcumin (10-5 M to 10-8 M) significantly (p<0.001) protects AChE activity in erythrocyte during oxidative stress. AChE activity in naïve membrane was found to be maximum at 10-6 M of curcumin. Curcumin at 10-6 M caused decrease in Km of the enzyme Our results signify the potential protective effect of curcumin on the cholinergic system under conditions of oxidative stress.
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Cite this article as:
Singh Prabhakar and Rizvi Syed Ibrahim, Curcumin Activates Erythrocyte Membrane Acetylcholinesterase, Letters in Drug Design & Discovery 2013; 10 (6) . https://dx.doi.org/10.2174/1570180811310060012
DOI https://dx.doi.org/10.2174/1570180811310060012 |
Print ISSN 1570-1808 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-628X |
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