Abstract
The aim of this study consisted in extracting and quantifying phenolic and flavonoids compounds of five selected Algerian plants. The second step was devoted to studying the effects of phenolic compounds on the kinetics catalyzed by two enzymes belonging to the class of hydrolase (the α - amylase and the α - glucosidase) responsible for the digestion of sugars. Finally, we assessed the potential antiradical of our extracts.
The results indicate that the phenolic extracts from these plants have inhibitory effects on both enzymes, with Ki values in µg/ml range (18.19-72.14 µg / ml) for the α - amylase and (52.26 - 203.90 µg / ml) for α – glucosidase. The antioxidant activity test shows that our phenolic extracts exhibit good antioxidant capacity comparatively to antioxidants taken as reference with IC50 values which vary from 0.044 µg / ml to 0.452 µg / ml. This work contributes to understanding the role of natural polyphenols in the regulation of oxidative stress and normalization of glycemic disorders.
Keywords: α- Amylase, antioxidant activity, DPPH, α- glucosidase, inhibition effect, phenolic extracts.
Current Enzyme Inhibition
Title:The Inhibitory Effect of Some Algerian Plants Phenolics Extracts on the α - glucosidase and α - amylase Activities and their Antioxidant Activities
Volume: 10 Issue: 1
Author(s): Ihcen Khacheba, Amar Djeridane, Abdelkarim Kameli and Mohamed Yousfi
Affiliation:
Keywords: α- Amylase, antioxidant activity, DPPH, α- glucosidase, inhibition effect, phenolic extracts.
Abstract: The aim of this study consisted in extracting and quantifying phenolic and flavonoids compounds of five selected Algerian plants. The second step was devoted to studying the effects of phenolic compounds on the kinetics catalyzed by two enzymes belonging to the class of hydrolase (the α - amylase and the α - glucosidase) responsible for the digestion of sugars. Finally, we assessed the potential antiradical of our extracts.
The results indicate that the phenolic extracts from these plants have inhibitory effects on both enzymes, with Ki values in µg/ml range (18.19-72.14 µg / ml) for the α - amylase and (52.26 - 203.90 µg / ml) for α – glucosidase. The antioxidant activity test shows that our phenolic extracts exhibit good antioxidant capacity comparatively to antioxidants taken as reference with IC50 values which vary from 0.044 µg / ml to 0.452 µg / ml. This work contributes to understanding the role of natural polyphenols in the regulation of oxidative stress and normalization of glycemic disorders.
Export Options
About this article
Cite this article as:
Khacheba Ihcen, Djeridane Amar, Kameli Abdelkarim and Yousfi Mohamed, The Inhibitory Effect of Some Algerian Plants Phenolics Extracts on the α - glucosidase and α - amylase Activities and their Antioxidant Activities, Current Enzyme Inhibition 2014; 10 (1) . https://dx.doi.org/10.2174/15734080113099990001
DOI https://dx.doi.org/10.2174/15734080113099990001 |
Print ISSN 1573-4080 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-6662 |
- Author Guidelines
- Graphical Abstracts
- Fabricating and Stating False Information
- Research Misconduct
- Post Publication Discussions and Corrections
- Publishing Ethics and Rectitude
- Increase Visibility of Your Article
- Archiving Policies
- Peer Review Workflow
- Order Your Article Before Print
- Promote Your Article
- Manuscript Transfer Facility
- Editorial Policies
- Allegations from Whistleblowers
Related Articles
-
Homocysteine, Cardiovascular Inflammation, and Myocardial Remodeling
Cardiovascular & Hematological Disorders-Drug Targets Identifying Risk Factors for Clinically Significant Diabetic Macula Edema in Patients with Type 2 Diabetes Mellitus
Current Diabetes Reviews Physiological Effect and Therapeutic Application of Alpha Lipoic Acid
Current Medicinal Chemistry Hypertension and Atrial Fibrillation: Any Change with the New Anticoagulants
Current Pharmaceutical Design The Prevalence and Progression of Arterial Calcification in Patients with End-Stage Renal Disease
Vascular Disease Prevention (Discontinued) Neuroprotection by (endo)Cannabinoids in Glaucoma and Retinal Neurodegenerative Diseases
Current Neuropharmacology Evidence-Based Percutaneous Closure of the Left Atrial Appendage in Patients with Atrial Fibrillation
Current Cardiology Reviews Algorithms and Criteria for Transcatheter Aortic Valve Replacement Patient Selection: Current Status and Future Trends
Current Pharmaceutical Design Functional Link Between Adenosine and Insulin: A Hypothesis for Fetoplacental Vascular Endothelial Dysfunction in Gestational Diabetes
Current Vascular Pharmacology Recent Clinical Aspects of Eating Disorders
Reviews on Recent Clinical Trials Oxidative-Nitrosative Stress as a Contributing Factor to Cardiovascular Disease in Subjects with Diabetes
Current Vascular Pharmacology Statistical Optimization of Pharmacogenomics Association Studies: Key Considerations from Study Design to Analysis
Current Pharmacogenomics and Personalized Medicine Hypersensitivity Reactions to Neuromuscular Blocking Agents
Current Pharmaceutical Design Nanoemulsions: A Better Approach for Antidiabetic Drug Delivery
Current Diabetes Reviews Relevance of Postprandial Lipemia in Metabolic Syndrome
Current Vascular Pharmacology Role of Endothelin in Diabetic Retinopathy
Current Vascular Pharmacology The Lung Disease of Rheumatoid Arthritis
Current Respiratory Medicine Reviews Role of Hyperphosphatemia on Cardiovascular Disease in Dialysis Patients
Vascular Disease Prevention (Discontinued) Genetic Signatures in the Treatment of Stroke
Current Pharmaceutical Design Micro-/Nano-Scale Biointerfaces, Mechanical Coupling and Cancer Therapy
Current Topics in Medicinal Chemistry