Abstract
Two different strategies were used to encapsulate oleanolic acid into nanoparticles, including nanoprecipitation and liposome technique. In nanoprecipitation method, the detailed formulations were investigated by varing the parameters of total good solvent/poor solvent volume ratio, surfactant mass ratio. The effects of different physical situations were considered in the experiment. The formulation which exhibits the most satisfactory colloidal stability and particles’ formation was identified. The average diameter of nanoparticles is about 150 nm shown by transmission electron microscopy (TEM). Secondly, proliposome and nano-sized liposomes were prepared. The liposomes contain a hydrophobic oleanolic acid core, an amphiphilic soybean lecithin monolayer and a hydrophilic PEG protective coating. They are dispersed individually and distributed around 110-140 nm in diameters. Encapsulation enficiencies (EE) of the two methods were calculated by high performance liquid chromatograpy. The EE of nanoparticles obtained are 86.7% and 92.6%, respectively. Furthermore, the stability of nanoparticles was explored in different physicochemical situations. The results demonstrate that nanoparticles can possess the higher stability at 4°C.
Keywords: Oleanolic acid, nanoprecipitation, nanoparticle, liposome, PEG, stability
Current Pharmaceutical Analysis
Title:Preparation and Characterization of Oleanolic Acid Nanoparticles
Volume: 9 Issue: 2
Author(s): Shengnan Tang, Jikui Hao, Dawei Gao, Jinting Duan and Zhiwei Liu
Affiliation:
Keywords: Oleanolic acid, nanoprecipitation, nanoparticle, liposome, PEG, stability
Abstract: Two different strategies were used to encapsulate oleanolic acid into nanoparticles, including nanoprecipitation and liposome technique. In nanoprecipitation method, the detailed formulations were investigated by varing the parameters of total good solvent/poor solvent volume ratio, surfactant mass ratio. The effects of different physical situations were considered in the experiment. The formulation which exhibits the most satisfactory colloidal stability and particles’ formation was identified. The average diameter of nanoparticles is about 150 nm shown by transmission electron microscopy (TEM). Secondly, proliposome and nano-sized liposomes were prepared. The liposomes contain a hydrophobic oleanolic acid core, an amphiphilic soybean lecithin monolayer and a hydrophilic PEG protective coating. They are dispersed individually and distributed around 110-140 nm in diameters. Encapsulation enficiencies (EE) of the two methods were calculated by high performance liquid chromatograpy. The EE of nanoparticles obtained are 86.7% and 92.6%, respectively. Furthermore, the stability of nanoparticles was explored in different physicochemical situations. The results demonstrate that nanoparticles can possess the higher stability at 4°C.
Export Options
About this article
Cite this article as:
Tang Shengnan, Hao Jikui, Gao Dawei, Duan Jinting and Liu Zhiwei, Preparation and Characterization of Oleanolic Acid Nanoparticles, Current Pharmaceutical Analysis 2013; 9 (2) . https://dx.doi.org/10.2174/1573412911309020008
DOI https://dx.doi.org/10.2174/1573412911309020008 |
Print ISSN 1573-4129 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-676X |
- Author Guidelines
- Graphical Abstracts
- Fabricating and Stating False Information
- Research Misconduct
- Post Publication Discussions and Corrections
- Publishing Ethics and Rectitude
- Increase Visibility of Your Article
- Archiving Policies
- Peer Review Workflow
- Order Your Article Before Print
- Promote Your Article
- Manuscript Transfer Facility
- Editorial Policies
- Allegations from Whistleblowers
- Announcements
Related Articles
-
A3 Receptor Ligands: Past, Present and Future Trends
Current Topics in Medicinal Chemistry Discovery of GPCR Ligands by Molecular Docking Screening: Novel Opportunities Provided by Crystal Structures
Current Topics in Medicinal Chemistry Editorial (Thematic Issue: Antitumor Alkylphospholipid Analogs: A Promising and Growing Family of Synthetic Cell Membrane-Targeting Molecules for Cancer Treatment)
Anti-Cancer Agents in Medicinal Chemistry Interleukin-15 in Gene Therapy of Cancer
Current Gene Therapy Evaluation of the Anthelmentic Activity of Garlic (Allium sativum) in Mice Naturally Infected with Aspiculuris tetraptera
Recent Patents on Anti-Infective Drug Discovery Meet Our Editorial Board Member
Current Drug Therapy Folding Versus Charge: Understanding Selective Target Recognition by the Thrombin Aptamers
Current Pharmaceutical Design Micro- and Macrovascular Treatment Targets in Scleroderma Heart Disease
Current Pharmaceutical Design Herbal Mixture Adsorbed to Polyethylene Glycol Microspheres Induces Apoptotic Effects on Breast Cancer Cells
Current Drug Delivery Statistical Analysis, Optimization, and Prioritization of Virtual Screening Parameters for Zinc Enzymes Including the Anthrax Toxin Lethal Factor
Current Topics in Medicinal Chemistry Diastereoselective Addition of Organometallic Reagents to Diimines Derived from (R,R)-1,2-Diaminocyclohexane and Aromatic Aldehydes
Letters in Organic Chemistry Transcription Factors as Targets for Cancer Therapy: AP-1 a Potential Therapeutic Target
Current Cancer Therapy Reviews The Regulation of miRNAs in Inflammation-Related Carcinogenesis
Current Pharmaceutical Design Platinum-Based Agents for Individualized Cancer Treatment
Current Molecular Medicine Clinical Pharmacology of Serotonin Receptor Type 3 (5-HT3) Antagonists
Current Medicinal Chemistry - Central Nervous System Agents Tracking Stem Cell Therapy in the Myocardium: Applications of Positron Emission Tomography
Current Pharmaceutical Design Systems Biology Approaches to a Rational Drug Discovery Paradigm
Current Topics in Medicinal Chemistry LPA and its Analogs-Attractive Tools for Elucidation of LPA Biology and Drug Development
Current Medicinal Chemistry Immuno-Isolation in Oncology - A Mini-Review
Current Pharmaceutical Biotechnology Synthesis and Anti-tumor Activity Evaluation of Gallic Acid-mangiferin Hybrid Molecule
Medicinal Chemistry