Vancomycin Microbial Assay Using Kinetic-Reading Microplate System

Title:Vancomycin Microbial Assay Using Kinetic-Reading Microplate System

Volume: 9 Issue: 2

Author(s): Tulia de Souza Botelho, Felipe Rebello Lourenco and Terezinha de Jesus. Andreoli Pinto

Affiliation:

Keywords: Agar diffusion, Antibiotic assay, Glycopeptide antibiotic, Kinetic reading, Method development, Method validation, Microbial assay, Microplate assay, Turbidimetric assay, Vancomycin

Abstract: The aim of this work was to develop a microbiological assay of vancomycin employing microplates and kinetic reading mode, and to validate the developed method by assessing of specificity and selectivity, linearity, linear range, accuracy and precision parameters. Established conditions comprised a vancomycin standard-curve in concentrations from 0.6 to 1.0 μg mL-1, and soybean-casein broth inoculated with 5% of Bacillus subtilis (ATCC 6633) suspension. Satisfactory results were obtained after a 5 hour incubation. The developed method showed appropriate selectivity and linearity in the range from 0.6 to 1.0 μg mL-1, respectively, satisfactory accuracy (recovery = 97.0%) and precision (RSD = 7.8%).

Cite this article as:

de Souza Botelho Tulia, Rebello Lourenco Felipe and de Jesus. Andreoli Pinto Terezinha, Vancomycin Microbial Assay Using Kinetic-Reading Microplate System, Current Pharmaceutical Analysis 2013; 9 (2) . https://dx.doi.org/10.2174/1573412911309020007