Abstract
A specific ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method was established and validated for the simultaneous quantification of paclitaxel prodrug (PP) and paclitaxel (PT) in human plasma. PP, PT and an internal standard 13C6-labelled PT (13C6-PT) were extracted from plasma by tert-butyl methyl ether and separated on an ACQUITY UPLC BEH C18 column (50 mm × 2.1 mm, 1.7 μm) using a mobile phase of methanolacetonitrile 50:50 (v/v) at a flow rate of 0.4 mL/min. At positive electrospray ionization mode, multiple reaction monitoring of the precursor-product ion transitions of m/z 882.2→313.9 for 13C6-PT, 876.2→307.9 for PT and 1216.5→647.8 for PP was used for the quantification. The linear calibration curve was obtained in a concentration range of 1-1000 ng/mL with a lower limit of quantification of 1 ng/mL. For both analytes, the values of intra- and inter-day precision were within 12.5% and accuracy fell in the ranges of 91.5-103.4%. The recovery ranged from 88.1% to 94.2% and the matrix effects from 89.3% to 95.4%. PP and PT were stable under short-term temperature and post-preparative conditions. The method was applied to stability of PP in human plasma and released PT from PP.
Keywords: Paclitaxel prodrug, paclitaxel, ultra-performance liquid chromatography-tandem mass spectrometry, stability in plasma
Current Pharmaceutical Analysis
Title:Simultaneous Quantification of Paclitaxel Prodrug and its Released Paclitaxel in Human Plasma by UPLC-MS/MS
Volume: 9 Issue: 2
Author(s): Shuang-Qing Zhang and Yu-Ming Fan
Affiliation:
Keywords: Paclitaxel prodrug, paclitaxel, ultra-performance liquid chromatography-tandem mass spectrometry, stability in plasma
Abstract: A specific ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method was established and validated for the simultaneous quantification of paclitaxel prodrug (PP) and paclitaxel (PT) in human plasma. PP, PT and an internal standard 13C6-labelled PT (13C6-PT) were extracted from plasma by tert-butyl methyl ether and separated on an ACQUITY UPLC BEH C18 column (50 mm × 2.1 mm, 1.7 μm) using a mobile phase of methanolacetonitrile 50:50 (v/v) at a flow rate of 0.4 mL/min. At positive electrospray ionization mode, multiple reaction monitoring of the precursor-product ion transitions of m/z 882.2→313.9 for 13C6-PT, 876.2→307.9 for PT and 1216.5→647.8 for PP was used for the quantification. The linear calibration curve was obtained in a concentration range of 1-1000 ng/mL with a lower limit of quantification of 1 ng/mL. For both analytes, the values of intra- and inter-day precision were within 12.5% and accuracy fell in the ranges of 91.5-103.4%. The recovery ranged from 88.1% to 94.2% and the matrix effects from 89.3% to 95.4%. PP and PT were stable under short-term temperature and post-preparative conditions. The method was applied to stability of PP in human plasma and released PT from PP.
Export Options
About this article
Cite this article as:
Zhang Shuang-Qing and Fan Yu-Ming, Simultaneous Quantification of Paclitaxel Prodrug and its Released Paclitaxel in Human Plasma by UPLC-MS/MS, Current Pharmaceutical Analysis 2013; 9 (2) . https://dx.doi.org/10.2174/1573412911309020005
DOI https://dx.doi.org/10.2174/1573412911309020005 |
Print ISSN 1573-4129 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-676X |
- Author Guidelines
- Graphical Abstracts
- Fabricating and Stating False Information
- Research Misconduct
- Post Publication Discussions and Corrections
- Publishing Ethics and Rectitude
- Increase Visibility of Your Article
- Archiving Policies
- Peer Review Workflow
- Order Your Article Before Print
- Promote Your Article
- Manuscript Transfer Facility
- Editorial Policies
- Allegations from Whistleblowers
- Announcements
Related Articles
-
Implications of PEGylation of Carbon Nanotubes for Central Nervous System Bioavailability
CNS & Neurological Disorders - Drug Targets Population Pharmacokinetic Evaluation of a Fully Human IgG2 Monoclonal Antibody in Patients with Inflammatory Diseases
Inflammation & Allergy - Drug Targets (Discontinued) Comparison of Artemisia annua Bioactivities between Traditional Medicine and Chemical Extracts
Current Bioactive Compounds Microemulsions for Colorectal Cancer Treatments. General Considerations and Formulation of Methotrexate
Mini-Reviews in Medicinal Chemistry Evaluation of Herbal Medicines: Value Addition to Traditional Medicines Through Metabolism, Pharmacokinetic and Safety Studies
Current Drug Metabolism In-silico Design, ADMET Screening, MM-GBSA Binding Free Energy of Some Novel Isoxazole Substituted 9-Anilinoacridines as HER2 Inhibitors Targeting Breast Cancer
Current Drug Research Reviews Isoliquiritigenin Inhibits Proliferation and Induces Apoptosis via Alleviating Hypoxia and Reducing Glycolysis in Mouse Melanoma B16F10 Cells
Recent Patents on Anti-Cancer Drug Discovery Antioxidant and Antifungal Potential of Pleurotus ostreatus and Agrocybe cylindracea Basidiocarps and Mycelia
Current Pharmaceutical Biotechnology Quantitative Structure – Activity Relationships (QSAR) with the MolNet Molecular Graph Machine
Current Bioinformatics Epitope Identification and Discovery Using Phage Display Libraries: Applications in Vaccine Development and Diagnostics
Current Drug Targets TNF-α Inhibitors with Anti-Oxidative Stress Activity from Natural Products
Current Topics in Medicinal Chemistry Glycan-Binding Profile and Cell Adhesion Activity of American Bullfrog (Rana catesbeiana) Oocyte Galectin-1
Protein & Peptide Letters A Comparative Study of Selected Drug Delivery Systems: Key Emphasis on Cocrystallization
Drug Delivery Letters Selenium and Selenoproteins: An Overview on Different Biological Systems
Current Protein & Peptide Science Three-Component Process for the Synthesis of Pyrimido[2,1-c][1,2,4]triazine Derivatives via Knoevenagel Condensation Under Thermal Aqueous Conditions
Current Organic Synthesis Functional Cell-Based Assays in Microliter Volumes for Ultra-High Throughput Screening
Combinatorial Chemistry & High Throughput Screening Nucleic Acid Carrier Systems Based on Polyethylenimine Conjugates for the Treatment of Metastatic Tumors
Current Medicinal Chemistry Nanoparticle-Based Diagnosis and Therapy
Current Drug Targets Review of the Third Domain Receptor Binding Fragment of Alphafetoprotein (AFP): Plausible Binding of AFP to Lysophospholipid Receptor Targets
Current Drug Targets Synthesis, Anticonvulsant and Antimicrobial Activities of Some New [1-(2-Naphthyl)-2-(pyrazol-1-yl)ethanone]oxime Ethers
Medicinal Chemistry