Abstract
Cyanobacteria produce oligopeptides that are predominantly synthesized by the non-ribosomal pathway. Among these are the aeruginosin and cyanopeptolin protease inhibitors, which act against enzymes known to cause several human health problems. Atomic force microscopy (AFM) was used to study the effect of cyanopeptides produced by Microcystis aeruginosa NPCD-1 on pathogenic bacterial cell surfaces. The selected strain was characterized based on the 16S rRNA gene sequence and the intergenic spacer region of the phycocyanin operon. PCR amplification was employed to investigate the presence of genes encoding for aeruginosin and cyanopeptolin. Purified extract from M. aeruginosa NPCD-1 cells was screened for bioactive compounds. The effect of purified extract containing protease inhibitors produced by the NPCD-1 strain on bacterial cells was observed using AFM. Aeruginosin and cyanopeptolin genes were confirmed by both PCR amplification and gene sequencing. Mass spectrometry analysis confirmed the production of aeruginosin. The interaction of Bacillus cereus, Escherichia coli and Staphylococcus aureus with cyanopeptides was characterized by examining the loss of surface stiffness and the formation of micelles, most likely originating from the membrane disruption. The AFM results demonstrate the ability of cyanobacterial extract to alter the cellular membrane of bacterial pathogens.
Keywords: Aeruginosin, atomic force microscopy, cyanobacteria, cyanopeptolin, mass spectrometry, protease inhibitor
Current Nanoscience
Title:Antimicrobial Cyanopeptide Action on Bacterial Cells Observed with Atomic Force Microscopy
Volume: 9 Issue: 1
Author(s): Maria Estela Silva-Stenico, Adriana Sturion Lorenzi, Omar Teschke, Caroline Souza Pamplona Silva, Augusto Etchegaray and Marli Fatima Fiore
Affiliation:
Keywords: Aeruginosin, atomic force microscopy, cyanobacteria, cyanopeptolin, mass spectrometry, protease inhibitor
Abstract: Cyanobacteria produce oligopeptides that are predominantly synthesized by the non-ribosomal pathway. Among these are the aeruginosin and cyanopeptolin protease inhibitors, which act against enzymes known to cause several human health problems. Atomic force microscopy (AFM) was used to study the effect of cyanopeptides produced by Microcystis aeruginosa NPCD-1 on pathogenic bacterial cell surfaces. The selected strain was characterized based on the 16S rRNA gene sequence and the intergenic spacer region of the phycocyanin operon. PCR amplification was employed to investigate the presence of genes encoding for aeruginosin and cyanopeptolin. Purified extract from M. aeruginosa NPCD-1 cells was screened for bioactive compounds. The effect of purified extract containing protease inhibitors produced by the NPCD-1 strain on bacterial cells was observed using AFM. Aeruginosin and cyanopeptolin genes were confirmed by both PCR amplification and gene sequencing. Mass spectrometry analysis confirmed the production of aeruginosin. The interaction of Bacillus cereus, Escherichia coli and Staphylococcus aureus with cyanopeptides was characterized by examining the loss of surface stiffness and the formation of micelles, most likely originating from the membrane disruption. The AFM results demonstrate the ability of cyanobacterial extract to alter the cellular membrane of bacterial pathogens.
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Cite this article as:
Estela Silva-Stenico Maria, Sturion Lorenzi Adriana, Teschke Omar, Souza Pamplona Silva Caroline, Etchegaray Augusto and Fatima Fiore Marli, Antimicrobial Cyanopeptide Action on Bacterial Cells Observed with Atomic Force Microscopy, Current Nanoscience 2013; 9 (1) . https://dx.doi.org/10.2174/1573413711309010024
DOI https://dx.doi.org/10.2174/1573413711309010024 |
Print ISSN 1573-4137 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-6786 |
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