Abstract
Free radical nitric oxide (NO) has been known to interact with various physiological processes, such as wound repair processes and control of vascular tone. However, NO is an unstable molecule and the development of NO delivery systems that enhance its stability has also been studied. In this work, alginate/chitosan nanoparticles have been studied as a drug delivery system of the Snitrosoglutathione (GSNO) as NO donor. For this, glutathione, GSH, the GSNO precursor, was encapsulated in alginate/chitosan nanoparticles. The presence of GSNO was confirmed by UV spectra at 336 nm. Alginate/chitosan nanoparticles with negative and positive surface charges were obtained by increasing the chitosan amount. The encapsulation efficiency (EE) relied on the nanoparticle zeta potential, obtaining 80% of EE for positive particles. The NO release from GSNO showed that polymeric nanoparticles lead to the stabilization of GSNO decomposition, at physiological temperature. Moreover, this system did not exhibit cytotoxicity for fibroblast V79 cells up to the maximum concentration tested (18 μmolL-1). These results showed that alginate/chitosan nanoparticles are interesting particles to encapsulate NO donors for biomedical applications where NO might have a therapeutic effect.
Keywords: Polymeric nanoparticles, alginate, chitosan, nitric oxide, glutathione, cytotoxicity, s-nitrosoglutathione, pharmacology
Current Nanoscience
Title:Development of a Sustained-release System for Nitric Oxide Delivery using Alginate/Chitosan Nanoparticles
Volume: 9 Issue: 1
Author(s): Priscyla D. Marcato, Leonardo F. Adami, Raquel de Melo Barbosa, Patricia S. Melo, Iasmin R. Ferreira, Larissa de Paula, Nelson Duran and Amedea B. Seabra
Affiliation:
Keywords: Polymeric nanoparticles, alginate, chitosan, nitric oxide, glutathione, cytotoxicity, s-nitrosoglutathione, pharmacology
Abstract: Free radical nitric oxide (NO) has been known to interact with various physiological processes, such as wound repair processes and control of vascular tone. However, NO is an unstable molecule and the development of NO delivery systems that enhance its stability has also been studied. In this work, alginate/chitosan nanoparticles have been studied as a drug delivery system of the Snitrosoglutathione (GSNO) as NO donor. For this, glutathione, GSH, the GSNO precursor, was encapsulated in alginate/chitosan nanoparticles. The presence of GSNO was confirmed by UV spectra at 336 nm. Alginate/chitosan nanoparticles with negative and positive surface charges were obtained by increasing the chitosan amount. The encapsulation efficiency (EE) relied on the nanoparticle zeta potential, obtaining 80% of EE for positive particles. The NO release from GSNO showed that polymeric nanoparticles lead to the stabilization of GSNO decomposition, at physiological temperature. Moreover, this system did not exhibit cytotoxicity for fibroblast V79 cells up to the maximum concentration tested (18 μmolL-1). These results showed that alginate/chitosan nanoparticles are interesting particles to encapsulate NO donors for biomedical applications where NO might have a therapeutic effect.
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Cite this article as:
D. Marcato Priscyla, F. Adami Leonardo, de Melo Barbosa Raquel, S. Melo Patricia, R. Ferreira Iasmin, de Paula Larissa, Duran Nelson and B. Seabra Amedea, Development of a Sustained-release System for Nitric Oxide Delivery using Alginate/Chitosan Nanoparticles, Current Nanoscience 2013; 9 (1) . https://dx.doi.org/10.2174/1573413711309010003
DOI https://dx.doi.org/10.2174/1573413711309010003 |
Print ISSN 1573-4137 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-6786 |
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