Abstract
An electrochemical microsensor has been developed for the simultaneous assay of ascorbate (AA) and urate (UA) antioxidants in human blood serum. The electrode surface was modified by means of electropolymerized conductive poly(3,4-ethylenedioxythiophene) PEDOT organic films. Highly selective responses (detection potential difference more than 250 mV) were obtained for both biomarkers by optimizing the PEDOT thickness. Contrary to most previous studies the analytical performances were recorded by testing the microsensor directly in blood serum without any pretreatment of the samples. Using differential pulse voltammetry (DPV) the sensitivity and detection limit were 0.481 μA μM-1 cm-2 and 4.2 μmol L-1 for AA and 1.815 μA μM-1 cm-2 and 2.0 μmol L-1 for UA. The calibration curves were linear in the concentration range 5–200 μmol L-1 for AA and 3-700 μmol L-1 for UA. The accuracy of the sensor was satisfactorily compared with the reference analytical methods provided that the synergic effect between both antioxidants was considered. The sensor response was unmodified in the presence of the major biochemical interfering species.
Keywords: Antioxidants, ascorbic and uric acids, blood serum analysis, EC’ mechanism, electrochemical microsensor, interfering species, PEDOT film, Oxidative stress, electropolymerization, μAu-PEDOT microsensor.
Combinatorial Chemistry & High Throughput Screening
Title:Simultaneous Assay of Ascorbate and Urate Antioxidants in Human Blood Serum Using PEDOT- Based Electrochemical Microsensor
Volume: 16 Issue: 2
Author(s): Fadhila Sekli-Belaidi, Anne Galinier and Pierre Gros
Affiliation:
Keywords: Antioxidants, ascorbic and uric acids, blood serum analysis, EC’ mechanism, electrochemical microsensor, interfering species, PEDOT film, Oxidative stress, electropolymerization, μAu-PEDOT microsensor.
Abstract: An electrochemical microsensor has been developed for the simultaneous assay of ascorbate (AA) and urate (UA) antioxidants in human blood serum. The electrode surface was modified by means of electropolymerized conductive poly(3,4-ethylenedioxythiophene) PEDOT organic films. Highly selective responses (detection potential difference more than 250 mV) were obtained for both biomarkers by optimizing the PEDOT thickness. Contrary to most previous studies the analytical performances were recorded by testing the microsensor directly in blood serum without any pretreatment of the samples. Using differential pulse voltammetry (DPV) the sensitivity and detection limit were 0.481 μA μM-1 cm-2 and 4.2 μmol L-1 for AA and 1.815 μA μM-1 cm-2 and 2.0 μmol L-1 for UA. The calibration curves were linear in the concentration range 5–200 μmol L-1 for AA and 3-700 μmol L-1 for UA. The accuracy of the sensor was satisfactorily compared with the reference analytical methods provided that the synergic effect between both antioxidants was considered. The sensor response was unmodified in the presence of the major biochemical interfering species.
Export Options
About this article
Cite this article as:
Sekli-Belaidi Fadhila, Galinier Anne and Gros Pierre, Simultaneous Assay of Ascorbate and Urate Antioxidants in Human Blood Serum Using PEDOT- Based Electrochemical Microsensor, Combinatorial Chemistry & High Throughput Screening 2013; 16 (2) . https://dx.doi.org/10.2174/1386207311316020002
DOI https://dx.doi.org/10.2174/1386207311316020002 |
Print ISSN 1386-2073 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-5402 |
Call for Papers in Thematic Issues
Artificial Intelligence Methods for Biomedical, Biochemical and Bioinformatics Problems
Recently, a large number of technologies based on artificial intelligence have been developed and applied to solve a diverse range of problems in the areas of biomedical, biochemical and bioinformatics problems. By utilizing powerful computing resources and massive amounts of data, methods based on artificial intelligence can significantly improve the ...read more
Eco-friendly Agents for Biological Control of Pathogenic Diseases
The discovery of an alternative biological approach to disease management includes work on medicinal products derived from natural sources as a starting point for the development of eco-friendly agents for these diseases and the injuries they cause, as well as reducing human contact with hazardous chemicals and their residues. We ...read more
Emerging trends in diseases mechanisms, noble drug targets and therapeutic strategies: focus on immunological and inflammatory disorders
Recently infectious and inflammatory diseases have been a key concern worldwide due to tremendous morbidity and mortality world Wide. Recent, nCOVID-9 pandemic is a good example for the emerging infectious disease outbreak. The world is facing many emerging and re-emerging diseases out breaks at present however, there is huge lack ...read more
Exploring Spectral Graph Theory in Combinatorial Chemistry
Scope of the Thematic Issue: Combinatorial chemistry involves the synthesis and analysis of a large number of diverse compounds simultaneously. Traditional methods rely on brute force experimentation, which can be time-consuming and resource-intensive. Spectral Graph Theory, a branch of mathematics dealing with the properties of graphs in relation to the ...read more
- Author Guidelines
- Graphical Abstracts
- Fabricating and Stating False Information
- Research Misconduct
- Post Publication Discussions and Corrections
- Publishing Ethics and Rectitude
- Increase Visibility of Your Article
- Archiving Policies
- Peer Review Workflow
- Order Your Article Before Print
- Promote Your Article
- Manuscript Transfer Facility
- Editorial Policies
- Allegations from Whistleblowers
Related Articles
-
Design and In vitro Validation of Multivalent Dendrimer Methotrexates as a Folate-targeting Anticancer Therapeutic
Current Pharmaceutical Design Recent Insights on the Medicinal Chemistry of Metal-Based Compounds: Hints for the Successful Drug Design
Current Medicinal Chemistry Apoptosis Following Photodynamic Tumor Therapy: Induction, Mechanisms and Detection
Current Pharmaceutical Design Design and Synthesis of Quinazolinone Derivatives as Anti-inflammatory Agents: Pharmacophore Modeling and 3D QSAR Studies
Medicinal Chemistry Role of Dietary Xenobiotics-Gene Interactions in Carcinogenesis: Protective Effects of Nutritional Factors
Current Nutrition & Food Science Magnetic Resonance Imaging of Uncommon Hepatic Mesenchymal Tumours: Haemangioendothelioma and Angiosarcoma
Current Medical Imaging Linear Poly(ethylenimine) Cross-Linked by Methyl-β-Cyclodextrin for Gene Delivery
Current Gene Therapy From Chemical Graphs in Computer-Aided Drug Design to General Markov-Galvez Indices of Drug-Target, Proteome, Drug-Parasitic Disease, Technological, and Social-Legal Networks
Current Computer-Aided Drug Design The Enzymology of Cytosolic Pyrimidine 5’-Nucleotidases: Functional Analysis and Physiopathological Implications.
Current Medicinal Chemistry Evaluation of Adhesion Force and Binding Affinity of Phytohemagglutinin Erythroagglutinating to EGF Receptor on Human Lung Cancer Cells
Current Medicinal Chemistry Mechanisms of Cytotoxicity of Anticancer Titanocenes
Anti-Cancer Agents in Medicinal Chemistry Cellulose-Supported Sulfonated Magnetic Nanoparticles: Utilized for One-pot Synthesis of α-Iminonitrile Derivatives
Current Organic Synthesis Inhibition of Human Aromatase by Myosmine
Drug Metabolism Letters Medicinal Importance, Pharmacological Activities and Analytical Aspects of Canadine in Medicine: An Important Phytochemical of <i>Hydrastis canadensis</i>
Current Chinese Chemistry Multi-target Pharmacological Effects of Platycodin D
Mini-Reviews in Organic Chemistry Thiol Proteases: Inhibitors and Potential Therapeutic Targets
Current Medicinal Chemistry Phenylalanine Hydroxylase Misfolding and Pharmacological Chaperones
Current Topics in Medicinal Chemistry SPR Imaging Biosensor for Aspartyl Cathepsins: Sensor Development and Application for Biological Material
Protein & Peptide Letters Diterpenoids from Liverworts and their Biological Activities
Current Organic Chemistry Electrochemical Behavior and Square-Wave Stripping Voltammetric Determination of Roflumilast in Pharmaceutical Dosage Forms
Combinatorial Chemistry & High Throughput Screening