Image Analysis of Colocalization of Nuclear DNA and GFP Labelled HIF-1α in Stable Transformants

Title:Image Analysis of Colocalization of Nuclear DNA and GFP Labelled HIF-1α in Stable Transformants

Volume: 13 Issue: 14

Author(s): Takehito Goto, Michihiko Sato, Eiji Takahashi and Yasutomo Nomura

Affiliation:

Keywords: HIF-1α, GFP, stable transformant, colocalization, cobalt, drug development, cancer, DRAQ5, dual labeling images, nuclear translocation, hypoxia response element (HRE), vessel, glycolytic enzymes, hypoxic

Abstract: HIF-1α is regarded as a target for drug development in several diseases such as cancer. For high throughput screening of HIF-1α-targeted drug, we need to examine the activity quantitatively. In the present study, we proposed a method where stable expression system of HIF-1α was combined with image correlation analysis. When the stable transformants were labeled with DRAQ5, we could detect Co2+-induced nuclear translocation by the use of cross-correlation analysis of the dual labeling images. In the case of high throughput screening for HIF-1α-targeted drug, we should use Pearson’s correlation coefficient to judge nuclear translocation.

Cite this article as:

Goto Takehito, Sato Michihiko, Takahashi Eiji and Nomura Yasutomo, Image Analysis of Colocalization of Nuclear DNA and GFP Labelled HIF-1α in Stable Transformants, Current Pharmaceutical Biotechnology 2012; 13 (14) . https://dx.doi.org/10.2174/138920101314151120122519