Abstract
Resonance light scattering technique has been employed to investigate the interaction between anthracyclines and DNA in the presence of quercetin. Upon binding to DNA, anthracyclines undergo dramatic enhancement in their resonance light scattering intensities, while quercetin goes through no obvious change in its intensity. Once being added into anthracyclines- DNA system, quercetin can induce great difference in resonance light scattering intensities between anthracyclines- DNA-quercetin ternary system and anthracyclines-DNA binary system. Consequently, the DNA binding abilities of anthracyclines can be improved by quercetin. Improved efficiency of quercetin on the interaction between anthracyclines and DNA has been calculated. Under the experimental condition, the improved efficiency values of doxorubicin, epirubicin and mitoxantrone were 38.78%, 28.53% and 16.69%, respectively. Additionally, the weak resonance light scattering intensity of quercetin-DNA system is greatly enhanced by anthracyclines and the enhanced intensity is proportional to the concentration of anthracyclines in the range 0.06-9.0 μg mL-1 for doxorubicin, 0.08-12 μg mL-1 for epirubicin and 0.01-3.0 μg mL-1 for mitoxantrone. The detection limits are 4.7 ng mL-1 for doxorubicin, 5.4 ng mL-1 for epirubicin and 1.4 ng mL-1 for mitoxantrone.
Keywords: Circular dichroism spectroscopy, DNA, Detection, Doxorubicin, Epirubicin, Interaction, Mitoxantrone, Quercetin, Resonance light scattering, Ultraviolet visible spectroscopy
Current Analytical Chemistry
Title:Investigation on the Interaction Between Anthracyclines and DNA in the Presence of Quercetin by Resonance Light Scattering Technique and Its Analytical Application
Volume: 9 Issue: 1
Author(s): Zhanguang Chen, Sihua Qian, Xi Chen, Junhui Chen, Guomin Zhang, Huiling Zhong
Affiliation:
Keywords: Circular dichroism spectroscopy, DNA, Detection, Doxorubicin, Epirubicin, Interaction, Mitoxantrone, Quercetin, Resonance light scattering, Ultraviolet visible spectroscopy
Abstract: Resonance light scattering technique has been employed to investigate the interaction between anthracyclines and DNA in the presence of quercetin. Upon binding to DNA, anthracyclines undergo dramatic enhancement in their resonance light scattering intensities, while quercetin goes through no obvious change in its intensity. Once being added into anthracyclines- DNA system, quercetin can induce great difference in resonance light scattering intensities between anthracyclines- DNA-quercetin ternary system and anthracyclines-DNA binary system. Consequently, the DNA binding abilities of anthracyclines can be improved by quercetin. Improved efficiency of quercetin on the interaction between anthracyclines and DNA has been calculated. Under the experimental condition, the improved efficiency values of doxorubicin, epirubicin and mitoxantrone were 38.78%, 28.53% and 16.69%, respectively. Additionally, the weak resonance light scattering intensity of quercetin-DNA system is greatly enhanced by anthracyclines and the enhanced intensity is proportional to the concentration of anthracyclines in the range 0.06-9.0 μg mL-1 for doxorubicin, 0.08-12 μg mL-1 for epirubicin and 0.01-3.0 μg mL-1 for mitoxantrone. The detection limits are 4.7 ng mL-1 for doxorubicin, 5.4 ng mL-1 for epirubicin and 1.4 ng mL-1 for mitoxantrone.
Export Options
About this article
Cite this article as:
Zhanguang Chen, Sihua Qian, Xi Chen, Junhui Chen, Guomin Zhang, Huiling Zhong , Investigation on the Interaction Between Anthracyclines and DNA in the Presence of Quercetin by Resonance Light Scattering Technique and Its Analytical Application, Current Analytical Chemistry 2013; 9 (1) . https://dx.doi.org/10.2174/1573411011309010135
DOI https://dx.doi.org/10.2174/1573411011309010135 |
Print ISSN 1573-4110 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-6727 |
Call for Papers in Thematic Issues
Advancements in biomedicine, corrosion inhibition/monitoring, and lubrication of carbon dots
Carbon dots, a class of carbon-based nanomaterials, have garnered substantial interest in various scientific and engineering fields due to their unique properties, including excellent optical properties, outstanding biocompatibility, easy preparation, good dispersity, ultrasmall size, low toxicity and environmental friendliness. This special issue of Current Analytical Chemistry aims to showcase cutting-edge ...read more
Analytical methods for environmental and food analysis
In recent years, the study of the chemical composition of food and the environment depends on analytical methods in order to acquire information about the chemical composition, processing, quality control and contamination of soil, water, air, plants and foods, aspects very important for food safety and environmental quality control. There ...read more
Applications of artificial intelligence in chemical analysis
Artificial intelligence (AI) has demonstrated its versatility in almost all scientific fields, especially analytical chemistry, which is leading the way in the integration of AI. According to Chemical Abstract Services, one of the major disciplines contributing to AI-related publications and patents is analytical chemistry. That?s because techniques such as deep ...read more
Bioanalytical and Biosensor Technologies for Target Molecules
Bioanalytical and biosensor technologies constitute the core of detecting and sensing diverse molecules within living organisms, encompassing proteins, DNA, small molecular metabolites, and beyond. These molecules assume pivotal roles in biological processes, rendering their analysis and sensing indispensable for comprehending biological phenomena and unraveling disease mechanisms. In the current landscape ...read more
- Author Guidelines
- Graphical Abstracts
- Fabricating and Stating False Information
- Research Misconduct
- Post Publication Discussions and Corrections
- Publishing Ethics and Rectitude
- Increase Visibility of Your Article
- Archiving Policies
- Peer Review Workflow
- Order Your Article Before Print
- Promote Your Article
- Manuscript Transfer Facility
- Editorial Policies
- Allegations from Whistleblowers
Related Articles
-
Prevention of Macrovascular Disease in Type 2 Diabetic Patients: Blockade of the Renin-Angiotensin-Aldosterone System
Current Diabetes Reviews The Role of Autophagy in Subarachnoid Hemorrhage: An Update
Current Neuropharmacology Quinone-Based Drugs: An Important Class of Molecules in Medicinal Chemistry
Medicinal Chemistry Diagnostic Cardiac Catheterization in the Pediatric Population
Current Cardiology Reviews The Woman’s Heart: Insights into New Potential Targeted Therapy
Current Medicinal Chemistry The Role of Protease-Activated Receptors for the Development of Myocarditis: Possible Therapeutic Implications
Current Pharmaceutical Design Rheumatoid Arthritis: Cardiovascular Manifestations, Pathogenesis, and Therapy
Current Pharmaceutical Design Targeting Generation of Antibodies Specific to Conformational Epitopes of Amyloid β-Derived Neurotoxins
CNS & Neurological Disorders - Drug Targets Outcomes of Patients with Chronic Heart Failure and Iron Deficiency Treated with Intravenous Iron: A Meta-analysis
Cardiovascular & Hematological Disorders-Drug Targets Phenotyping and Genotyping of the Ryanodine Receptor-Associated Genetic Diseases Using Peripheral Lymphocytes
Current Pharmacogenomics Tracking Stem Cell Therapy in the Myocardium: Applications of Positron Emission Tomography
Current Pharmaceutical Design Diabetic Retinopathy and Atherosclerosis: is there a Link?
Current Diabetes Reviews Connexin43 and Myocardial Ischemia-Reperfusion Injury
Cardiovascular & Hematological Disorders-Drug Targets Inflammatory Biomarkers and Therapeutic Targets in Heart Failure
Current Medicinal Chemistry Takotsubo Syndrome: Clinical Manifestations, Etiology and Pathogenesis
Current Cardiology Reviews Editorial (Thematic Issues: Pharmacological Mechanisms and Interventions in Ischemia/ Reperfusion-Induced Injury)
Current Pharmaceutical Design Targeting Resveratrol to Mitochondria for Cardiovascular Diseases
Recent Patents on Cardiovascular Drug Discovery Metabolic Syndrome and Myocardial Infarction in Women
Current Pharmaceutical Design Flavonoids as Potential Therapeutic Agents for the Management of Diabetic Neuropathy
Current Pharmaceutical Design Editorial (Thematic Issue: Managing Strategies for Diverse Diseases: Challenges from Bench to Bedside Translation in Successful Drug Discovery and Development (Part B))
Current Pharmaceutical Design