Regulation of DNA Replication Licensing

Hiroyuki      Niida; Masatoshi      Kitagawa

Abstract

In eukaryotic cells, DNA replication is tightly regulated to occur only once per cell cycle. DNA licensing is a mechanism to guarantee this aim; that is, licensing of replication initiation is permitted during late M phase to G1 phase. The license is canceled by the start of DNA replication. Once DNA replication begins, the license is never given until the next late M phase. The licensing corresponds to the process of assembling components of the pre-replication complex (pre-RC) on the replication origin DNA. This pre-RC is the target of several different regulation systems to prevent rereplication of DNA during a single cell cycle. In this review, the regulation mechanisms mainly in mammals to control assembling components of the pre-RC will be discussed.

Keywords: CDC10-dependent transcript 1 (CDT1), cell division cycle 6 (CDC6), DNA replication, high mobility group AThook 1 (HMGA1), histone acetyltransferase binding to ORC (HBO1), homeobox protein Hox-D13 (HOXD13), licensing, mini chromosome maintenance (MCM), origin recognition complex (ORC), prereplication complex (pre-RC), CDC10-dependent transcript 1 (CDT1), cell division cycle 6 (CDC6), DNA replication, high mobility group AThook1 (HMGA1), histone acetyltransferase binding to ORC (HBO1), homeobox protein Hox-D13 (HOXD13), licensing, mini chromosome maintenance (MCM), origin recognition complex (ORC), prereplication complex (pre-RC)