Formulation and Evaluation of in vitro Cytotoxicity of Carboplatin Niosomes

V.      Sankar; I.      R. Antony Noby; B.      Ramakrishna; Elizabeth      Babu; Shalini      Devi Penmetsa; Sivaram      Hariharan

Abstract

The goal of our study was to prepare carboplatin niosomes for enhanced delivery to cancer cells by thin film hydration technique using (cholesterol: surfactant) in the micromolar ratio of 30:100 and with/without the addition of charge inducing agents and Pluronic F 68. Photomicrographs of the formulations show the presence of multilamellar vesicles in the forumulation with Tween 80 and Pluronic F 68. The vesicle size of the formulation was found to be in the size range of 0.01-1µm. Carboplatin niosome formulation without any charge inducing agent and Pluronic F68 shows good drug entrapment (89.24%) with an in vitro release of 87.23% in 12 hours. Drug release kinetics of the formulation follows the Non Fickian diffusion mechanism and exhibits first order release kinetics. The in vitro cytotoxicity results of the formulations reveal that the Tween 80 formulation with Pluronic shows the highest level of cytotoxicity (90%) when compared with drug in solution. Also, the stability studies of the formulations reveal that the formulations were stable in refrigerated condition.

Keywords: Carboplatin, Cancer and Niosomes, in vitro Cytotoxicity, Drug targeting, therapeutic, immunoglobulins, serum proteins, synthetic polymers, liposomes, microspheres, erythrocytes, phospholipid, nonionic surfactant, viruses.