Generic placeholder image

Current Drug Metabolism

Editor-in-Chief

ISSN (Print): 1389-2002
ISSN (Online): 1875-5453

Strategies for Improving the Quantitative Bioanalytical Performance of LC-MS in Pharmacokinetic Studies

Author(s): Li Li, Dandan Tian, Feng Chen, Junling Yang, Ke Yu and Yan Sun

Volume 13, Issue 9, 2012

Page: [1206 - 1212] Pages: 7

DOI: 10.2174/138920012803341320

Price: $65

Abstract

Quantitative bioanalysis is urgently required for the evaluation of pharmacokinetic properties of a drug and to demonstrate the body exposure to the parent drug and/or metabolite for interpretation of the efficacy and toxicity. New trends in drug discovery and development will be always posing challenges on LC–MS-based quantitative bioanalysis. The focus of this minireview is to highlight the commonly used strategies for improving the quantitative bioanalytical performance including overcoming matrix effects and improving MS detectability. “LC-electrolyte effects” and “pulse gradient chromatography” proposed by our group are new approaches that have also showed potential efficiencies on improving overall bioassay performance, including lowering lower limit of quantification (LLOQ), enlarging upper limit of quantification (ULOQ), decreasing matrix effects, and overcoming elutropic effects, etc.. They should also work well in metabolic profiling studies and other important analytical fields, such as food pesticide residue analysis, environmental analysis, clinical and forensic toxicology, doping control, and so on.

Keywords: Pharmacokinetics, LC–MS, matrix effects, sensitivity enhancement, LC-electrolyte effects, pulse gradient chromatography


Rights & Permissions Print Cite
© 2024 Bentham Science Publishers | Privacy Policy