Abstract
Cocaine and crack usage is a major public health concern worldwide, mainly when the target consumers are pregnant women. The purpose of this study was to develop and validate a cost-effective method for identification and quantification of cocaine, benzoylecgonine, cocaethylene, and anhydroecgonine methyl ester in meconium samples, using solid-phase extraction (SPE) and GC/MS (ion trap – full scan mode) to be employed in public hospitals in Brazil. The method was validated in the 20 - 1000 ng/g range for cocaine and cocaethylene, 40 - 1500 ng/g for benzoylecgonine and 60 - 1500 ng/g for anhydroecgonine methyl ester, using 0.5 g meconium per assay. The detector response was linear in the studied range, and the limit of detection was found to be 10 ng/g for cocaine and cocaethylene, 30 ng/g for benzoylecgonine, and 40 ng/g for anhydroecgonine methyl ester. Intra-batch coefficients of variation oscillated between 3.01% and 10.15% and inter-batch coefficients varied between 5.31% and 11.12%; accuracy was in 91.47% - 105.31% range. Recoveries were higher than 56.30%. Finally, the method was applied to meconium analysis from 20 newborns at the Hospital Clínicas da Faculdade de Medicina de Ribeirão Preto, and it identified 6 positive samples.
Keywords: Cocaine and metabolities, Crack, Gas chromatography/mass spectrometry, Meconium, Method validation, Solid phase extraction
Current Pharmaceutical Analysis
Title:Validation of a Solid Phase Extraction Procedure for Identification and Quantification of Cocaine and Metabolities in Meconium Using GC/MS
Volume: 8 Issue: 4
Author(s): Marcela N. R. Alves, Geraldo Duarte, Marisa M. M. Pinhata and Bruno S. De Martinis
Affiliation:
Keywords: Cocaine and metabolities, Crack, Gas chromatography/mass spectrometry, Meconium, Method validation, Solid phase extraction
Abstract: Cocaine and crack usage is a major public health concern worldwide, mainly when the target consumers are pregnant women. The purpose of this study was to develop and validate a cost-effective method for identification and quantification of cocaine, benzoylecgonine, cocaethylene, and anhydroecgonine methyl ester in meconium samples, using solid-phase extraction (SPE) and GC/MS (ion trap – full scan mode) to be employed in public hospitals in Brazil. The method was validated in the 20 - 1000 ng/g range for cocaine and cocaethylene, 40 - 1500 ng/g for benzoylecgonine and 60 - 1500 ng/g for anhydroecgonine methyl ester, using 0.5 g meconium per assay. The detector response was linear in the studied range, and the limit of detection was found to be 10 ng/g for cocaine and cocaethylene, 30 ng/g for benzoylecgonine, and 40 ng/g for anhydroecgonine methyl ester. Intra-batch coefficients of variation oscillated between 3.01% and 10.15% and inter-batch coefficients varied between 5.31% and 11.12%; accuracy was in 91.47% - 105.31% range. Recoveries were higher than 56.30%. Finally, the method was applied to meconium analysis from 20 newborns at the Hospital Clínicas da Faculdade de Medicina de Ribeirão Preto, and it identified 6 positive samples.
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Cite this article as:
N. R. Alves Marcela, Duarte Geraldo, M. M. Pinhata Marisa and S. De Martinis Bruno, Validation of a Solid Phase Extraction Procedure for Identification and Quantification of Cocaine and Metabolities in Meconium Using GC/MS, Current Pharmaceutical Analysis 2012; 8 (4) . https://dx.doi.org/10.2174/157341212803341618
DOI https://dx.doi.org/10.2174/157341212803341618 |
Print ISSN 1573-4129 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-676X |
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