Abstract
CAMA-syn, a hybrid composed of N-terminal α-helical segment of Cecropin A(amino acid 1-8) and Magainin 2 (amino acid 1-12), is a novel small peptide with the potent antibacterial and synergistic activity without cytotoxicity. In order to test the antibacterial function of CAMA-syn produced in mammalian cells, several vectors containing the synthesized CAMA-syn DNA fragment were constructed and transfected into recipient cells. The results showed that CAMAsyn fusion to green fluorescent protein (GFP) or to hemagglutinin epitope (HA) tag was expressed in both bovine embryo fibroblasts (BEF) and mouse macrophage RAW264.7 cells. The antibacterial assays of CAMA-syn were conducted against both Gram positive and negative bacteria, including S. abortusovis, P. anatis, S. hyicus and S. suis. The results of colony-forming efficiency and cell growth curves proved that the in vitro expressed CAMA-syn could have the antibacterial activity, demonstrating that macrophage specific expression of antimicrobial peptide CAMA-syn could inhibit the growth of bacteria.
Keywords: Antibacterial assay, antimicrobial peptide, bovine fibroblast, CAMA-syn, macrophage, α-helical, cytotoxicity, hemagglutinin epitope
Protein & Peptide Letters
Title:Functional Analysis of Hybrid Peptide CAMA-Syn: Expression in Mammalian Cells and Antimicrobial Potential
Volume: 19 Issue: 10
Author(s): Junlin Zhang, Sha Peng, Xiang Cheng and Huayan Wang
Affiliation:
Keywords: Antibacterial assay, antimicrobial peptide, bovine fibroblast, CAMA-syn, macrophage, α-helical, cytotoxicity, hemagglutinin epitope
Abstract: CAMA-syn, a hybrid composed of N-terminal α-helical segment of Cecropin A(amino acid 1-8) and Magainin 2 (amino acid 1-12), is a novel small peptide with the potent antibacterial and synergistic activity without cytotoxicity. In order to test the antibacterial function of CAMA-syn produced in mammalian cells, several vectors containing the synthesized CAMA-syn DNA fragment were constructed and transfected into recipient cells. The results showed that CAMAsyn fusion to green fluorescent protein (GFP) or to hemagglutinin epitope (HA) tag was expressed in both bovine embryo fibroblasts (BEF) and mouse macrophage RAW264.7 cells. The antibacterial assays of CAMA-syn were conducted against both Gram positive and negative bacteria, including S. abortusovis, P. anatis, S. hyicus and S. suis. The results of colony-forming efficiency and cell growth curves proved that the in vitro expressed CAMA-syn could have the antibacterial activity, demonstrating that macrophage specific expression of antimicrobial peptide CAMA-syn could inhibit the growth of bacteria.
Export Options
About this article
Cite this article as:
Zhang Junlin, Peng Sha, Cheng Xiang and Wang Huayan, Functional Analysis of Hybrid Peptide CAMA-Syn: Expression in Mammalian Cells and Antimicrobial Potential, Protein & Peptide Letters 2012; 19 (10) . https://dx.doi.org/10.2174/092986612802762705
DOI https://dx.doi.org/10.2174/092986612802762705 |
Print ISSN 0929-8665 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-5305 |
- Author Guidelines
- Graphical Abstracts
- Fabricating and Stating False Information
- Research Misconduct
- Post Publication Discussions and Corrections
- Publishing Ethics and Rectitude
- Increase Visibility of Your Article
- Archiving Policies
- Peer Review Workflow
- Order Your Article Before Print
- Promote Your Article
- Manuscript Transfer Facility
- Editorial Policies
- Allegations from Whistleblowers
Related Articles
-
Diabetes Mellitus in Saudi Arabia: A Review of the Recent Literature
Current Diabetes Reviews Natural Compounds with Proteasome Inhibitory Activity for Cancer Prevention and Treatment
Current Protein & Peptide Science Protein and Peptide Biopharmaceuticals: An Overview
Protein & Peptide Letters Post Marketing Surveillance on Safety and Efficacy of IMOD in Iranian Patients with HIV/AIDS
Infectious Disorders - Drug Targets The Relationships between the Transforming Growth Factor-β 1, Age and the Ultrasound Parameters of Arterial Wall
Vascular Disease Prevention (Discontinued) Cannabis Use and Psychosis: Theme Introduction
Current Pharmaceutical Design Inhibition of Lethal Endotoxin Shock with an L-Pyridylalanine Containing Metalloproteinase Inhibitor Selected by High-Throughput Screening of a New Peptide Library
Combinatorial Chemistry & High Throughput Screening RNA Interference as A Gene-Specific Approach for Molecular Medicine
Current Medicinal Chemistry Vitamin A in Prevention of Bronchopulmonary Dysplasia
Current Pharmaceutical Design Microglia in Alzheimer’s Disease
Current Alzheimer Research Anti-Inflammatory Properties of Acetylcholinesterase Inhibitors Administred in Alzheimers Disease
Anti-Inflammatory & Anti-Allergy Agents in Medicinal Chemistry When Neighbors Talk: Colon Cancer Cell Invasion and Tumor Microenvironment Myofibroblasts
Current Drug Targets Isoform Selective Phosphoinositide 3-Kinase γ and δ Inhibitors and Their Therapeutic Potential
Recent Patents on Inflammation & Allergy Drug Discovery Therapeutic Strategies of Plant-derived Compounds for Diabetes Via Regulation of Monocyte Chemoattractant Protein-1
Current Medicinal Chemistry Patent Selections
Recent Patents on CNS Drug Discovery (Discontinued) Recent Development in the Search for Effective Antidepressants Using Traditional Chinese Medicine
Central Nervous System Agents in Medicinal Chemistry Aspects of Antisense Oligodeoxynucleotide, Ribozyme, DNA Enzyme and RNAi Design
Current Medicinal Chemistry - Central Nervous System Agents Structure and Function of the Type III Secretion System of Pseudomonas aeruginosa
Current Protein & Peptide Science Meet Our Editor-in-Chief
CNS & Neurological Disorders - Drug Targets Computer Prediction of Cardiovascular and Hematological Agents by Statistical Learning Methods
Cardiovascular & Hematological Agents in Medicinal Chemistry