Abstract
An electrochemical biosensor was developed based on formaldehyde dehydrogenase immobilized with Nafion membrane for determination of formaldehyde in fish. The enzyme was immobilized through the entrapment technique and measured based on the reduction of &bgr;-nicotinamide adenine dinucleotide. The response time of the formaldehyde biosensor was <1 min, with an optimum pH of 8. The optimum enzyme loading and NAD+ concentrations were found at 30 mg/mL and 0.5 mM, respectively. Using the formaldehyde biosensor, a linear response of formaldehyde showed a range of 0.1 to 10 ppm and a detection limit of 0.016 ppm. In application of Nash method, the samples were stored at 4°C ± 1 for 10 days. With the two combined methods, a linear correlation coefficient with R2 = 0.9982 (y = 0.956x - 0.014) was found. The developed formaldehyde biosensor showed a good reproducibility, long storage stability (more than 6 months stored at 4°C), and also effective monitoring of formaldehyde level in Indian mackerel (Rastrelliger kanagurta) fish.
Keywords: Electrochemical biosensor, Formaldehyde dehydrogenase, Formaldehyde, Nafion, Indian Mackerel.
Current Analytical Chemistry
Title:Electrochemical Biosensor Immobilization of Formaldehyde Dehydrogenase with Nafion for Determination of Formaldehyde from Indian Mackerel (Rastrelliger kanagurta) Fish
Volume: 8 Issue: 4
Author(s): NurIndang Marzuki, Fatimah Abu Bakar, Abu Bakar Salleh, Lee Yook Heng, Nor Azah Yusof and Shafiquzzaman Siddiquee
Affiliation:
Keywords: Electrochemical biosensor, Formaldehyde dehydrogenase, Formaldehyde, Nafion, Indian Mackerel.
Abstract: An electrochemical biosensor was developed based on formaldehyde dehydrogenase immobilized with Nafion membrane for determination of formaldehyde in fish. The enzyme was immobilized through the entrapment technique and measured based on the reduction of &bgr;-nicotinamide adenine dinucleotide. The response time of the formaldehyde biosensor was <1 min, with an optimum pH of 8. The optimum enzyme loading and NAD+ concentrations were found at 30 mg/mL and 0.5 mM, respectively. Using the formaldehyde biosensor, a linear response of formaldehyde showed a range of 0.1 to 10 ppm and a detection limit of 0.016 ppm. In application of Nash method, the samples were stored at 4°C ± 1 for 10 days. With the two combined methods, a linear correlation coefficient with R2 = 0.9982 (y = 0.956x - 0.014) was found. The developed formaldehyde biosensor showed a good reproducibility, long storage stability (more than 6 months stored at 4°C), and also effective monitoring of formaldehyde level in Indian mackerel (Rastrelliger kanagurta) fish.
Export Options
About this article
Cite this article as:
Marzuki NurIndang, Bakar Abu Fatimah, Salleh Bakar Abu, Heng Yook Lee, Yusof Azah Nor and Siddiquee Shafiquzzaman, Electrochemical Biosensor Immobilization of Formaldehyde Dehydrogenase with Nafion for Determination of Formaldehyde from Indian Mackerel (Rastrelliger kanagurta) Fish, Current Analytical Chemistry 2012; 8 (4) . https://dx.doi.org/10.2174/157341112803216843
DOI https://dx.doi.org/10.2174/157341112803216843 |
Print ISSN 1573-4110 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-6727 |
Call for Papers in Thematic Issues
Advancements in biomedicine, corrosion inhibition/monitoring, and lubrication of carbon dots
Carbon dots, a class of carbon-based nanomaterials, have garnered substantial interest in various scientific and engineering fields due to their unique properties, including excellent optical properties, outstanding biocompatibility, easy preparation, good dispersity, ultrasmall size, low toxicity and environmental friendliness. This special issue of Current Analytical Chemistry aims to showcase cutting-edge ...read more
Advances in Microfluidic and Sensing Technologies of Biofluids.
Detection of biological fluids is an important means to assess human health and diagnose diseases. Currently, research on blood is relatively mature and widely applied in the medical field. With the development and advancement of microfluidic technology and novel detection methods, attention has turned to human physiological fluids such as ...read more
Analytical methods for environmental and food analysis
In recent years, the study of the chemical composition of food and the environment depends on analytical methods in order to acquire information about the chemical composition, processing, quality control and contamination of soil, water, air, plants and foods, aspects very important for food safety and environmental quality control. There ...read more
Applications of artificial intelligence in chemical analysis
Artificial intelligence (AI) has demonstrated its versatility in almost all scientific fields, especially analytical chemistry, which is leading the way in the integration of AI. According to Chemical Abstract Services, one of the major disciplines contributing to AI-related publications and patents is analytical chemistry. That?s because techniques such as deep ...read more
- Author Guidelines
- Graphical Abstracts
- Fabricating and Stating False Information
- Research Misconduct
- Post Publication Discussions and Corrections
- Publishing Ethics and Rectitude
- Increase Visibility of Your Article
- Archiving Policies
- Peer Review Workflow
- Order Your Article Before Print
- Promote Your Article
- Manuscript Transfer Facility
- Editorial Policies
- Allegations from Whistleblowers
Related Articles
-
Design, Synthesis and Molecular Modeling Studies of Some Heterocyclic Compounds Derived from the Suzuki-coupling of 6-bromo-1,3,4-oxadiazine Together with their Antitumor and Anti-leishmanial Evaluations
Current Organic Chemistry Combination of Phytochemicals as Adjuvants for Cancer Therapy
Recent Patents on Anti-Cancer Drug Discovery Targeted Delivery of siRNA Therapeutics using Ligand Mediated Biodegradable Polymeric Nanocarriers
Current Pharmaceutical Design A Perspective on Clinical Islet Transplantation: Past, Present and Developments for Future
Immunology, Endocrine & Metabolic Agents in Medicinal Chemistry (Discontinued) Multiple Protective Functions of Sigma1 Receptor
Current Protein & Peptide Science State of the Art Clinical Efficacy and Safety Evaluation of N-Acetylcarnosine Dipeptide Ophthalmic Prodrug. Principles for the Delivery, Self-Bioactivation, Molecular Targets and Interaction with a Highly Evolved Histidyl-Hydrazide Structure in the Treatment and Therapeutic Management of a Group of Sight-Threatening Eye Diseases
Current Clinical Pharmacology Analysis of Current Antifungal Agents and Their Targets within the Pneumocystis carinii Genome
Current Drug Targets Neuroimaging of the Serotonin Transporter: Possibilities and Pitfalls
Current Psychiatry Reviews Seeing the Light: Luminescent Reporter Gene Assays
Combinatorial Chemistry & High Throughput Screening Patent Selections:
Recent Patents on Drug Delivery & Formulation Multiscale Mapping of AIDS in U.S. Countries vs Anti-HIV Drugs Activity with Complex Networks and Information Indices
Current Bioinformatics Factors Promoting Tamoxifen Resistance in Breast Cancer via Stimulating Breast Cancer Stem Cell Expansion
Current Medicinal Chemistry Epigenetic Modifications Due to Heavy Metals Exposure in Children Living in Polluted Areas
Current Genomics Tubulin Folding Pathways: Implication in the Regulation of Microtubule Dynamics
Current Cancer Drug Targets Therapeutic Options and Emerging Alternatives for Multidrug Resistant Staphylococcal Infections
Current Pharmaceutical Design Towards Improved Therapies Using Nanopharmaceuticals: Recent Patents on Pharmaceutical Nanoformulations
Recent Patents on Food, Nutrition & Agriculture Modulators of Networks: Molecular Targets of Arterial Calcification Identified in Man and Mice
Current Pharmaceutical Design Novel Nanotechnology Approaches for Targeted Cancer Therapy
Recent Patents on Nanomedicine High-Throughput Screening Assays to Discover Small-Molecule Inhibitors of Protein Interactions
Current Drug Discovery Technologies Therapeutic Modulation of Cytokines in Chronic Infectious Diseases
Current Pharmaceutical Design