Abstract
Translational research is necessary for the development of efficient experimental animal models that can be used to develop innovative medical treatments, such as improvements in organ or tissue transplantation. We have developed animal models that produce photogenic proteins in their islet cells: rats models expressing the gene for luciferase or green fluorescent protein (GFP), and pig models expressing the gene for GFP or Kusabira-Orange. We also developed methods for preserving isolated islets in culture and showed that the fluorescence of the islets remains at usable levels for at least seven days. These models will enable transplanted islets to be visualized without the need for chemical reactions, and will be useful for research on the biology of islets as well as for the development of new transplantation methods.
Keywords: Islets, Diabetes, Preservation, Transgenic animals, Rat, Pig, Imaging, Cell transplantation, green fluorescent protein, Kusabira-Orange
Current Diabetes Reviews
Title:Islets From Rats and Pigs Transgenic for Photogenic Proteins
Volume: 8 Issue: 5
Author(s): Takumi Teratani, Hitomi Matsunari, Naoya Kasahara, Hiroshi Nagashima, Tatsuo Kawarasaki and Eiji Kobayashi
Affiliation:
Keywords: Islets, Diabetes, Preservation, Transgenic animals, Rat, Pig, Imaging, Cell transplantation, green fluorescent protein, Kusabira-Orange
Abstract: Translational research is necessary for the development of efficient experimental animal models that can be used to develop innovative medical treatments, such as improvements in organ or tissue transplantation. We have developed animal models that produce photogenic proteins in their islet cells: rats models expressing the gene for luciferase or green fluorescent protein (GFP), and pig models expressing the gene for GFP or Kusabira-Orange. We also developed methods for preserving isolated islets in culture and showed that the fluorescence of the islets remains at usable levels for at least seven days. These models will enable transplanted islets to be visualized without the need for chemical reactions, and will be useful for research on the biology of islets as well as for the development of new transplantation methods.
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Cite this article as:
Teratani Takumi, Matsunari Hitomi, Kasahara Naoya, Nagashima Hiroshi, Kawarasaki Tatsuo and Kobayashi Eiji, Islets From Rats and Pigs Transgenic for Photogenic Proteins, Current Diabetes Reviews 2012; 8 (5) . https://dx.doi.org/10.2174/157339912802083504
DOI https://dx.doi.org/10.2174/157339912802083504 |
Print ISSN 1573-3998 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-6417 |
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