Abstract
Amyloid plaque is associated with several neuronal and non-neuronal degenerative diseases. More than twenty human proteins can fold abnormally to form pathological deposits like amyloid plaque. Strategies for treating such diseases include therapies designed to decrease protein plaque formation or its complete clearance, but monitoring/clinical trials of these treatments are limited by the lack of effective methods to monitor amyloid deposits in the organs/tissues of living patients. The current study shows binding and staining ability of quinacrine to protein amyloid deposits, using Hen Egg White Lysozyme (HEWL) as model system and characterization of its binding interaction with HEWL, employing several biophysical techniques. Since quinacrine can pass the blood brain barrier, the current report suggests potential application of quinacrine for antemortem diagnostic of amyloid.
Keywords: Amyloid plaque, fluorescence quenching, FTIR, hen egg white lysozyme, imaging, quinacrine, fibrillar protein, Alzheimer’s disease, Parkinson’s Disease, Type II Diabetes Mellitus
Protein & Peptide Letters
Title:Evaluating Quinacrine as a Potential Amyloid Imaging Compound: Studies on Hen Egg White Lysozyme as Model System
Volume: 19 Issue: 8
Author(s): Manjeet Kumar, Nandini Sarkar and Vikash Kumar Dubey
Affiliation:
Keywords: Amyloid plaque, fluorescence quenching, FTIR, hen egg white lysozyme, imaging, quinacrine, fibrillar protein, Alzheimer’s disease, Parkinson’s Disease, Type II Diabetes Mellitus
Abstract: Amyloid plaque is associated with several neuronal and non-neuronal degenerative diseases. More than twenty human proteins can fold abnormally to form pathological deposits like amyloid plaque. Strategies for treating such diseases include therapies designed to decrease protein plaque formation or its complete clearance, but monitoring/clinical trials of these treatments are limited by the lack of effective methods to monitor amyloid deposits in the organs/tissues of living patients. The current study shows binding and staining ability of quinacrine to protein amyloid deposits, using Hen Egg White Lysozyme (HEWL) as model system and characterization of its binding interaction with HEWL, employing several biophysical techniques. Since quinacrine can pass the blood brain barrier, the current report suggests potential application of quinacrine for antemortem diagnostic of amyloid.
Export Options
About this article
Cite this article as:
Kumar Manjeet, Sarkar Nandini and Kumar Dubey Vikash, Evaluating Quinacrine as a Potential Amyloid Imaging Compound: Studies on Hen Egg White Lysozyme as Model System, Protein & Peptide Letters 2012; 19 (8) . https://dx.doi.org/10.2174/092986612801619598
DOI https://dx.doi.org/10.2174/092986612801619598 |
Print ISSN 0929-8665 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-5305 |
- Author Guidelines
- Graphical Abstracts
- Fabricating and Stating False Information
- Research Misconduct
- Post Publication Discussions and Corrections
- Publishing Ethics and Rectitude
- Increase Visibility of Your Article
- Archiving Policies
- Peer Review Workflow
- Order Your Article Before Print
- Promote Your Article
- Manuscript Transfer Facility
- Editorial Policies
- Allegations from Whistleblowers
Related Articles
-
Exploring the Cardio-metabolic Relevance of T-cadherin: A Pleiotropic Adiponectin Receptor
Endocrine, Metabolic & Immune Disorders - Drug Targets Fibroblast Growth Factor: Promising Target for Schizophrenia
Current Drug Targets Exosomes and Exosomal microRNAs in Age-associated Stroke
Current Vascular Pharmacology Schiff Bases of Istain: Antiglycation Activity
Letters in Drug Design & Discovery Soluble CD40L and its Role in Essential Hypertension: Diagnostic and Therapeutic Implications
Cardiovascular & Hematological Disorders-Drug Targets Antidiabetic Drugs as Antihypertensives: New Data on the Horizon
Current Vascular Pharmacology Diabetes Mellitus: A Potential Target for Stem Cell Therapy
Current Stem Cell Research & Therapy Anti-Diabetic Effect of a Flavonoid and Sitosterol - Rich Aqueous Extract of <i>Pleurotus tuberregium</i> Sclerotia in Alloxan-Induced Diabetic Rabbits
Endocrine, Metabolic & Immune Disorders - Drug Targets Unique Coronary Artery Disease Differences in Women as Related to Revascularization
Current Medicinal Chemistry Editorial: Alzheimer's Disease: From Molecular Mechanisms to Psychobiological Perspectives
Current Alzheimer Research Structural and Functional Characterization of Pathogenic Non- Synonymous Genetic Mutations of Human Insulin-Degrading Enzyme by In Silico Methods
CNS & Neurological Disorders - Drug Targets Cerebral Arachidonate Cascade in Dementia: Alzheimers Disease and Vascular Dementia
Current Neuropharmacology Statins as Either Immunomodulators or Anti-Cancer Drugs: Functional Activities on Tumor Stromal Cells and Natural Killer Cells
Anti-Inflammatory & Anti-Allergy Agents in Medicinal Chemistry Current Update on Synopsis of miRNA Dysregulation in Neurological Disorders
CNS & Neurological Disorders - Drug Targets Effective Management of the Type 2 Diabetes Patient with Cardiovascular and Renal Disease: Secondary Prevention Strategies after a Myocardial Infarction
Current Diabetes Reviews Dyslipidemia as a Risk Factor for Ischemic Stroke
Current Topics in Medicinal Chemistry Nicotinamide: Oversight of Metabolic Dysfunction Through SIRT1, mTOR, and Clock Genes
Current Neurovascular Research Mangiferin – a Bioactive Xanthonoid, not only from Mango and not just Antioxidant
Mini-Reviews in Medicinal Chemistry Non-Demented Individuals with Alzheimer’s Disease Neuropathology: Resistance to Cognitive Decline May Reveal New Treatment Strategies
Current Pharmaceutical Design Diabetes Association with Liver Diseases: An Overview for Clinicians
Endocrine, Metabolic & Immune Disorders - Drug Targets