Imidazole and 1,2,4-Triazole-based Derivatives Gifted with Antitubercular Activity: Cytotoxicity and Computational Assessment
Background: Mycobacterium Tuberculosis (Mtb) is the causative pathogen of Tuberculosis
(TB) and outbreaks are more common among immunosuppressed persons infected with HIV. The current
treatment regimens are lengthy and toxic, yet the therapy has remained unchanged for many decades,
so there is a need to find new structures with selective mechanism of action. Moreover, the increased
incidence of severe disseminated infections produced by undiagnosed Multidrug-resistant
(MDR), worsen clinical treatment and contribute the spread of the disease.
Objective: The aim of our study was to evaluate the potential of imidazole and triazole moieties for
antimycobacterial activity, by synthesizing some 1-(1-(aryl)-2-(2,6-dichlorophenyl)hydrazono)ethyl-
1H-imidazole and 1H-1,2,4-triazole derivatives 2a-l.
Methods: The title compounds were obtained via classical organic synthesis. The antimicrobial activity
was evaluated using the method of microdilution and the cytotoxicity assay was performed by MTT
Results: The results indicated that the presence of both the imidazole ring and that of the 2,6-
dichlorosubstituted phenyl moiety, is more relevant for inhibitory activity against Mtb than the triazole
nucleus and the unsubstituted phenyl ring. Among the series, (E)-1-(2-(5-chlorothiophen-2-yl)-2-(2-
(2,6-dichlorophenyl)hydrazono)ethyl)-1H-imidazole derivative 2f and (Z)-1-(2-([1,1’-biphenyl]-4-yl)-
2-(2-(2,6-dichlorophenyl)hydrazono)ethyl]-1H-imidazole derivatives 2e exhibited a promising antimycobacterial
property and the latter also displayed a safe cytotoxic profile.
Conclusion: The synthesized compounds were studied for their antitubercular activity. Among the
series, the compounds 2e and 2f appeared to be the most promising agents and, according to the docking
assessment, the compounds could be CYP51 inhibitors. These evidences could be useful for the
future development of new antimycobacterial derivatives targeting CYP51 with more specificity for the
mycobacterial cell enzyme.Journal Title:
Current Topics in Medicinal Chemistry