Bioinformatics Study of RNA Interference on the Effect of HIF-1α on Apelin Expression in Nasopharyngeal Carcinoma Cells
Background: HIF-1α can affect the apelin expression and participates in the
developments in cancers but the mechanism need to be explored further.
Objective: This paper investigates apelin expression in nasopharyngeal carcinoma CNE-2 cells and
its regulation by hypoxia inducible factor-1α (HIF-1α) under hypoxic conditions.
Methods: CoCl2 was used to induce hypoxia in CNE-2 cells for 12h, 24h and 48h. HIF-1α small
interference RNA (siRNA) was transfected into CNE-2 cells using a transient transfection method.
HIF-1α and apelin mRNA levels were detected by real time PCR. Western blot was used to
measure HIF-1α protein expression. The concentration of apelin in cell culture supernatant was
determined by enzyme linked immunosorbent assay (ELISA).
Results: HIF-1α and apelin mRNA levels and protein expression in CNE-2 cells increased
gradually with increased duration of hypoxic exposure and were significantly reduced in HIF-1α
siRNA transfected cells exposed to the same hypoxic conditions.
Conclusion: Apelin expression is induced by hypoxia and regulated by HIF-1α in CNE-2 cells.Journal Title: