Generic placeholder image

Current Bioactive Compounds

Editor-in-Chief

ISSN (Print): 1573-4072
ISSN (Online): 1875-6646

Research Article

GCMS Analysis of Bioactives of Piper betle Linn. Leaf

Author(s): Sapna Saini, Sanju Nanda and Anju Dhiman*

Volume 16 , Issue 1 , 2020

Page: [24 - 32] Pages: 9

DOI: 10.2174/1573407215666190417153613

Price: $65

Abstract

Background: Piper betle Linn. (Piperaceae) is commonly known as “Green gold of India” due to its high commercial value. It is a Vedic plant used in various traditional system of medicine v.iz. Ayurveda system of medicine, Yunani system of medicine attributed to various pharmacological activities v.i.z. antioxidant, anti-inflammatory, analgesic, anti-fertility, antimutagenic etc.

Objectives: The main objective of the present study is quantitative estimation of the various bioactive compounds present in leaf of P. betle L. (Bangla variety) by GC-MS analysis using head space method.

Materials and Methods: The vapor sample of leaf of P. betle L. was prepared by head space method for GCMS analysis. GCMS analysis was carried out using high resolution Thermo scientific TSQ 8000 Gas Chromatograph-Mass Spectrometer. Helium is used as carrier gas (99.999 %) with a constant flow rate of 1 ml/min. Total run time was 29.10 min.

Results: GC-MS analysis revealed the presence of 16 bioactive compounds in leaf of P. betle L. Eugenol (29.13%), Bakuchiol (11.79%) and α-linalool (14.88%) are the chief bioactive compounds of leaf. Leaf has been also reported to contain a good percentage of various terpenoids like β- caryophyllene (7.69), α-muurolene (4.19), α-bisabolene (0.98), etc.

Conclusion: From GC-MS analysis results it can be concluded that leaf of P. betle L. is a reservoir of valuable phenolic and susquiterpene compounds. Eugenol chief biomarker compound present leaf is a versatile remarkable molecule. Due to number of phytoconstituents P. betle L. leaf is recommended as plant of phytopharmaceutical importance.

Keywords: Antioxidant, antimicrobial, eugenol, GCMS, Piper betle, phytocomponents.

Graphical Abstract
[1]
Das, S.; Parida, R.; Sriram Sandeep, I.; Nayak, S.; Mohanty, S. Biotechnological intervention in betelvine (Piper betle L.): A review on recent advances and future prospects. Asian Pac. J. Trop. Med., 2016, 9(10), 938-946.
[http://dx.doi.org/10.1016/j.apjtm.2016.07.029] [PMID: 27794386]
[2]
Bhalerao, S.A.; Verma, D.R.; Gavankar, R.V.; Teli, N.C.; Rane, Y.Y.; Didwana, V.S. Phytochemistry, pharmacological profile and therapeutic uses of Piper betle Linn. An overview. J. Pharmacog. Phytochem., 2013, 1(2), 10-19.
[3]
Pradhan, D.; Suri, K.A.; Pradhan, D.K.; Biswasroy, P. Golden heart of the nature: Piper betle L. J. Pharmacog. Phytochem., 2013, 1(6), 147-167.
[4]
Dwivedi, V.; Tripathi, S. Review study on potential activity of Piper betle. J. Pharmacog. Phytochem., 2014, 3(4), 93-98.
[5]
Deshpande, S.N.; Kadam, D.G. GCMS analysis and antibacterial activity of Piper betle (Linn.) leaves against Streptococcus mutans. Asian J. Pharm. Clin. Res., 2013, 6(5), 99-101.
[6]
Chakraborty, D.; Shah, B. Antimicrobial, anti-oxidative and anti-hemolytic activity of Piper betle leaf extracts. Int. J. Pharm. Pharm. Sci., 2011, 3(3), 192-199.
[7]
Alam, B.; Majumder, R.; Akter, S.; Lee, S.H. Piper betle extracts exhibit antitumor activity by augmenting antioxidant potential. Oncol. Lett., 2015, 9(2), 863-868.
[http://dx.doi.org/10.3892/ol.2014.2738] [PMID: 25624910]
[8]
Arambewela, L.S.; Arawwawala, L.D.; Ratnasooriya, W.D. Antidiabetic activities of aqueous and ethanolic extracts of Piper betle leaves in rats. J. Ethnopharmacol., 2005, 102(2), 239-245.
[9]
Biswal, S. Phytochemical analysis and a study on the antiestrogenic antifertility effect of leaves of Piper betel in female albino rat. Anc. Sci. Life, 2014, 34(1), 16-22.
[http://dx.doi.org/10.4103/0257-7941.150770] [PMID: 25737606]
[10]
Saini, S.; Dhiman, A.; Nanda, S. Pharmacognostical and phytochemical studies of Piper betle Linn. leaf. Int. J. Pharm. Pharm. Sci., 2016, 8(5), 222-226.
[11]
Bihana, S.; Dhiman, A.; Singh, G.; Satija, S. Gas chromatography-mass spectroscopy analysis of bioactive compounds in the whole plant parts of ethanolic extract of Asclepias curassavica L; Int. J. Green Pharm, 2018, pp. 107-116.
[12]
Deshpande, S.N. GCMS analysis and antibacterial acivity of Piper betle (Linn.) leaves against Streptococcus mutans. Asian J. Pharm. Clin. Res., 2013, 6(5), 99-101.
[13]
Foo, L.W.; Salleh, E.; Mamat, S.N.H. Extraction and qualitative analysis of Piper betle leaves for antimicrobial activities. Int. J. Engg. Tech. Sci. Res., 2015, 2, 1-8.
[14]
Raja, M.R.C.; Srinivasan, V.; Selvaraj, S.; Mahapatra, S.K. Versatile and synergistic potential of eugenol: A review. Pharm. Anal. Acta, 2015, 6(5), 1-6.
[15]
Devi, K.P.; Nisha, S.A.; Sakthivel, R.; Pandian, S.K. Eugenol (an essential oil of clove) acts as an antibacterial agent against Salmonella typhi by disrupting the cellular membrane. J. Ethnopharmacol., 2010, 130(1), 107-115.
[http://dx.doi.org/10.1016/j.jep.2010.04.025] [PMID: 20435121]
[16]
Peana, A.T.; D’Aquila, P.S.; Panin, F.; Serra, G.; Pippia, P.; Moretti, M.D. Anti-inflammatory activity of linalool and linalyl acetate constituents of essential oils. Phytomedicine, 2002, 9(8), 721-726.
[http://dx.doi.org/10.1078/094471102321621322] [PMID: 12587692]
[17]
Chaudhuri, R.K.; Marchio, F. Bakuchiol in the management of acne-affected skin. Cosmet. Toiletries Mag., 2011, 126(7), 502-510.
[18]
Lin, J.; Yao, H.J.; Li, R.Y. Bakuchiol inhibits cell proliferation and induces apoptosis and cell cycle arrest in SGC-7901 human gastric cancer cells. J. BUON, 2016, 21(4), 889-894.
[PMID: 27685910]
[19]
Vijayalaxmi, A.; Bakshi, V.; Begum, N.; Kowmudi, V.; Kumar, N.Y.; Reddy, Y. Anti-arthritic and anti-inflammatory activity of beta-caryophyllene against freund’s complete adjuvant induced arthritis in Wistar rats. J. Bone Rep. Recomm., 2015, 1(29), 1-8.
[20]
Chaudhary, J.; Kaur, N.; Jain, A.; Kishore, L. Stigmasterol: A comprehensive review. Int. J. Pharm. Sci. Res., 2011, 2(9), 2259-2265.
[21]
Martins, Cde.M do Nascimento, E.A.; de Morais, S.A.; de Oliveira, A.; Chang, R.; Cunha, L.C.S.; Martins, M.M.; Martins, C.H.; Moraes, Tda. S.; Rodrigues, P.V.; da Silva, C.V.; de Aquino, F.J. Chemical constituents and evaluation of antimicrobial and cytotoxic activities of Kielmeyera coriacea Mart. & Zucc. Essential oils. Evid. Based Complement. Alternat. Med., 2015, 2015 842047
[http://dx.doi.org/10.1155/2015/842047] [PMID: 25960759]
[22]
Park, S.N.; Lim, Y.K.; Freire, M.O.; Cho, E.; Jin, D.; Kook, J.K. Antimicrobial effect of linalool and α-terpineol against periodontopathic and cariogenic bacteria. Anaerobe, 2012, 18(3), 369-372.
[http://dx.doi.org/10.1016/j.anaerobe.2012.04.001] [PMID: 22537719]
[23]
Miguel, M.G. Antioxidant and anti-inflammatory activities of essential oils: A short review. Molecules, 2010, 15(12), 9252-9287.
[http://dx.doi.org/10.3390/molecules15129252] [PMID: 21160452]
[24]
Dr. Duke’s phytochemical and ethnobotanical databases https://phytochem.nal.usda.gov/phytochem/chemicals/show/16611?et (Assessed September 15, 2017.)
[25]
Caboni, P.; Ntalli, N.G.; Aissani, N.; Cavoski, I.; Angioni, A. Nematicidal activity of (E,E)-2,4-decadienal and (E)-2-decenal from Ailanthus altissima against Meloidogyne javanica. J. Agric. Food Chem., 2012, 60(4), 1146-1151.
[http://dx.doi.org/10.1021/jf2044586] [PMID: 22224661]
[26]
Pavithra, B. Eugenol: a review. J. Pharm. Sci. Res., 2014, 6(14), 153-154.
[27]
Hassanpouraghdam, M.B.; Tabatabaie, S.J.; Nazemiyeh, H.; Vojodi, L.; Aazami, M.A.; Shoja, A.M. Chrysanthemum balsamita (L.) baill.: A forgotten medicinal plant. Facta Universitatis Series: Med. Biol., 2008, 15(3), 119-124.
[28]
Yeo, S.K.; Ali, A.Y.; Hayward, O.A.; Turnham, D.; Jackson, T.; Bowen, I.D.; Clarkson, R. β-Bisabolene, a sesquiterpene from the essential oil extract of opoponax (Commiphora guidottii), exhibits cytotoxicity in breast cancer cell lines. Phytother. Res., 2016, 30(3), 418-425.
[http://dx.doi.org/10.1002/ptr.5543] [PMID: 26666387]
[29]
Ivanescu, B.; Lungu, C.; Spac, A.; Tuchilu, C. Essential oils from Amorpha fruticosa L. fruits-chemical characterization and antimicrobial activity. Biologie Vegetala, 2014, 60(1), 33-39.
[30]
Hsu, H.C.; Yang, W.C.; Tsai, W.J.; Chen, C.C.; Huang, H.Y.; Tsai, Y.C. α-bulnesene, a novel PAF receptor antagonist isolated from Pogostemon cablin. Biochem. Biophys. Res. Commun., 2006, 345(3), 1033-1038.
[http://dx.doi.org/10.1016/j.bbrc.2006.05.006] [PMID: 16712790]
[31]
Vijisaral, E.D.; Arumugam, S. GC-MS analysis of bioactive constituents of Indigofera suffruticosa leaves. J. Chem. Pharm. Res., 2014, 6(8), 294-300.
[32]
Madrid, A.; Cardile, V.; González, C.; Montenegro, I.; Villena, J.; Caggia, S.; Graziano, A.; Russo, A. Psoralea glandulosa as a potential source of anticancer agents for melanoma treatment. Int. J. Mol. Sci., 2015, 16(4), 7944-7959.
[http://dx.doi.org/10.3390/ijms16047944] [PMID: 25860949]
[33]
Kiplimo, J.J. A review on the biological activity and the triterpenoids from the genus vernonia (asteraceae family). Int. Res. J. Pure Appl. Chem., 2016, 11(3), 1-14.
[http://dx.doi.org/10.9734/IRJPAC/2016/25091]

Rights & Permissions Print Export Cite as
© 2022 Bentham Science Publishers | Privacy Policy