Research Article

血液循环外泌体中含有游离细胞和细胞表面相关囊泡的可区分部分

卷 19, 期 4, 2019

页: [273 - 285] 页: 13

弟呕挨: 10.2174/1566524019666190314120532

价格: $65

摘要

背景:考虑到外泌体作为细胞间转运蛋白,不可避免地与质膜和大量可用的血细胞表面相互作用,我们想知道一小部分循环外泌体是否与血细胞表面有关。 目的:本研究的目的是开发一种有效的方案,用于分离与血细胞表面相关的外泌体,并进一步研究该部分在健康状态和乳腺癌发展过程中的特征,以及其可能的意义。用于诊断应用。 方法:从健康女性(HF)和乳腺癌患者(BCP)收集血液样本。从血浆中提取的外泌体和从血细胞表面洗脱的外体通过超滤分离,随后进行超速离心。 结果:透射电子显微镜(TEM)以及免疫金标记证明,从血浆和血细胞洗脱液中分离的膜包裹的细胞外囊泡(EV)中存在外泌体。 TEM,纳米粒子追踪分析和NanoOrange蛋白质定量数据显示,细胞相关外泌体构成不少于总血液外泌体数量的2/3。大约50-70纳米的外泌体在乳腺癌患者的血液中占优势,而较小的外泌体(30-50纳米)主要在健康女性的血液中观察到。对在血液中循环的外来体中的特定蛋白质和RNA的分析证明了HF和BCP的外来体中聚合物的堆积密度的显着差异。初步数据表明,检测与红细胞分数相关的外泌体中的癌症特异性miRNA(miR-103,miR-191,miR-195),与血浆中循环的无细胞外泌体相比,可以更准确地区分HF和BCP。 。 结论:我们的数据为使用血细胞相关外泌体进行诊断应用提供了基础。

关键词: 外泌体,细胞外囊泡,血细胞,细胞相关外泌体,miRNA,乳腺癌。

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