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Current Protein & Peptide Science


ISSN (Print): 1389-2037
ISSN (Online): 1875-5550

Review Article

Biotechnological Applications of Protein Splicing

Author(s): Corina Sarmiento and Julio A. Camarero*

Volume 20 , Issue 5 , 2019

Page: [408 - 424] Pages: 17

DOI: 10.2174/1389203720666190208110416

Price: $65


Protein splicing domains, also called inteins, have become a powerful biotechnological tool for applications involving molecular biology and protein engineering. Early applications of inteins focused on self-cleaving affinity tags, generation of recombinant polypeptide α-thioesters for the production of semisynthetic proteins and backbone cyclized polypeptides. The discovery of naturallyoccurring split-inteins has allowed the development of novel approaches for the selective modification of proteins both in vitro and in vivo. This review gives a general introduction to protein splicing with a focus on their role in expanding the applications of intein-based technologies in protein engineering and chemical biology.

Keywords: Inteins, split-inteins, protein modification, protein trans-splicing, protein splicing, post-translational modification, conditional protein splicing, backbone cyclized proteins.

Graphical Abstract
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