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Recent Patents on Biotechnology


ISSN (Print): 1872-2083
ISSN (Online): 2212-4012

Research Article

Ammonia Sensing and Cytotoxicity of the Biosynthesized Silver Nanoparticle by Arabic Gum (AG)

Author(s): Nahid Jalalian Elahi, Masoud Salehmoghadam, Danial Taherzadeh, Alireza Hashemzadeh and Majid Darroudi*

Volume 13 , Issue 3 , 2019

Page: [228 - 238] Pages: 11

DOI: 10.2174/1872208313666190118123141

Price: $65


Background: The green synthesizing procedure of Silver nanoparticles (AgNPs) has been performed through the usage of a natural polysaccharide; Arabic gum (AG) as a stabilizing/ capping agent. For characterization of AgNPs, TEM, particle size analyzer and UV-Vis were used.

Methods: The aim of our project was to identify biosynthesized AgNPs for sensing ammonia and to explore its toxicity on Neuro-2A cells. We also reviewed the patents for biosynthesized AgNO3 and ammonia sensing. The optimal conditions for the synthesis of AgNPs in AG consist of utilizing (0.1g) AG in dH2O (70 ml), 10 ml of 1 mM silver solution and 0.1 mM (AA) at 70°C stirring for 30 minutes. The AgNPs cytotoxicity was evaluated on Neuro-2A cells; consequently, ammonia was sensed with the lowest possible concentration of 10-6.

Results: Particle size analyzer displayed the mean diameter of about 70 nm for the sphericalshaped Ag-NPs. UV–Vis revealed that the prepared AgNPs were ammonia sensitive in solution as the concentration of ammonia was increased. The cytotoxicity of AgNPs indicated lower Cell viability at higher concentrations of the AG-capped AgNPs.

Conclusion: By synthesis of AgNPs in GA by using AA, we successfully prepared a sensor to diagnose ammonia in a cell and sensing its level at concentrations of 10-6 M. In this study, no therapeutic application has been shown, but this method could be utilized industrially for therapeutic purposes in the future.

Keywords: Silver nanoparticles, Arabic gum, stabilizing/capping agent, cytotoxicity, MTT assay, ammonia.

Graphical Abstract
Ensafi AA, Karimi-Maleh H. Modified multiwall carbon nanotubes paste electrode as a sensor for simultaneous determination of 6-thioguanine and folic acid using ferrocenedicarboxylic acid as a mediator. J Electroanal Chem 2010; 640: 75-83.
Krasteva N, Besnard I, Guse B, et al. Self-assembled gold nanoparticle/dendrimer composite films for vapor sensing applications. Nano Lett 2002; 2: 551-5.
Prow T, Smith JN, Grebe R, et al. Construction, gene delivery, and expression of DNA tethered nanoparticles. Mol Vis 2006; 12: 606-15.
Abdel-Halim E, El-Rafie M, Al-Deyab SS. Polyacrylamide/guar gum graft copolymer for preparation of silver nanoparticles. Carbohydr Polym 2011; 85: 692-7.
Malinsky MD, Kelly KL, Schatz GC, Van Duyne RP. Chain length dependence and sensing capabilities of the localized surface plasmon resonance of silver nanoparticles chemically modified with alkanethiol self-assembled monolayers. J Am Chem Soc 2001; 123: 1471-82.
Sherry LJ, Chang S-H, Schatz GC, Van Duyne RP, Wiley BJ, Xia Y. Localized surface plasmon resonance spectroscopy of single silver nanocubes. Nano Lett 2005; 5: 2034-8.
Dubas ST, Pimpan V. Green synthesis of silver nanoparticles for ammonia sensing. Talanta 2008; 76: 29-33.
Guo H, Tao S. Silver nanoparticles doped silica nanocomposites coated on an optical fiber for ammonia sensing. Sens Actuators B 2007; 123: 578-82.
Pandey S, Goswami GK, Nanda KK. Green synthesis of biopolymer–silver nanoparticle nanocomposite: an optical sensor for ammonia detection. Int J Biol Macromol 2012; 51: 583-9.
Tran QT, Hoa HTM, Yoo D-H, et al. Reduced graphene oxide as an over-coating layer on silver nanostructures for detecting nh 3 gas at room temperature. Sens Actuators B 2014; 194: 45-50.
Otamiri M, Nilsson KG. Analysis of human serum antibody–carbohydrate interaction using biosensor based on surface plasmon resonance. Int J Biol Macromol 1999; 26: 263-8.
Hua L, Chen J, Ge L, Tan SN. Silver nanoparticles as matrix for laser desorption/ionization mass spectrometry of peptides. J Nanopart Res 2007; 9: 1133-8.
Xiong D, Chen M, Li H. Synthesis of para-sulfonatocalix [4] arene-modified silver nanoparticles as colorimetric histidine probes. Chem Commun 2008; 7: 880-2.
Jiang Z, Chen Y, Liang A, Tao H, Tang N, Zhong F. Silver nanoparticle labeled immunoresonance scattering spectral assay for trace fibrinogen. Sci China Ser B: Chem 2007; 50: 345-50.
Xu X-HN, Brownlow WJ, Kyriacou SV, Wan Q, Viola JJ. Real-time probing of membrane transport in living microbial cells using single nanoparticle optics and living cell imaging. Biochem 2004; 43: 10400-13.
Huo S-J, Xue X-K, Li Q-X, Xu S-F, Cai W-B. Seeded-growth approach to fabrication of silver nanoparticle films on silicon for electrochemical atr surface-enhanced ir absorption spectroscopy. J Phys Chem B 2006; 110: 25721-8.
Wiley BJ, Chen Y, McLellan JM, et al. Synthesis and optical properties of silver nanobars and nanorice. Nano Lett 2007; 7: 1032-6.
Dubas ST, Pimpan V. Humic acid assisted synthesis of silver nanoparticles and its application to herbicide detection. Mater Lett 2008; 62: 2661-3.
Mishra Y, Mohapatra S, Kabiraj D, et al. Synthesis and characterization of ag nanoparticles in silica matrix by atom beam sputtering. Scr Mater 2007; 56: 629-32.
Sharma VK, Yngard RA, Lin Y. Silver nanoparticles: green synthesis and their antimicrobial activities. Adv Colloid Interface Sci 2009; 145: 83-96.
Luo C, Zhang Y, Zeng X, Zeng Y, Wang Y. The role of poly (ethylene glycol) in the formation of silver nanoparticles. J Colloid Interface Sci 2005; 288: 444-8.
Zheng M, Gu M, Jin Y, Jin G. Optical properties of silver-dispersed pvp thin film. Mater Res Bull 2001; 36: 853-9.
Zhang Z, Zhang L, Wang S, Chen W, Lei Y. A convenient route to polyacrylonitrile/silver nanoparticle composite by simultaneous polymerization–reduction approach. Polym 2001; 42: 8315-8.
Singh N, Khanna P. In situ synthesis of silver nano-particles in polymethylmethacrylate. Mater Chem Phys 2007; 104: 367-72.
Khanna P, Singh N, Charan S, Viswanath AK. Synthesis of ag/polyaniline nanocomposite via an in situ photo-redox mechanism. Mater Chem Phys 2005; 92: 214-9.
Zhou Y, Yu SH, Wang CY, Li XG, Zhu YR, Chen ZY. A novel ultraviolet irradiation photoreduction technique for the preparation of single‐crystal ag nanorods and ag dendrites. Adv Mater 1999; 11: 850-2.
Joseph S, Mathew B. Microwave-assisted facile synthesis of silver nanoparticles in aqueous medium and investigation of their catalytic and antibacterial activities. J Mol Liq 2014; 197: 346-52.
Bakar NA, Ismail J, Bakar MA. Synthesis and characterization of silver nanoparticles in natural rubber. Mater Chem Phys 2007; 104: 276-83.
Huang H, Yang X. Synthesis of polysaccharide-stabilized gold and silver nanoparticles: A green method. Carbohydr Res 2004; 339: 2627-31.
Cai J, Kimura S, Wada M, Kuga S. Nanoporous cellulose as metal nanoparticles support. Biomacromolecules 2008; 10: 87-94.
Kemp MM, Kumar A, Mousa S, et al. Gold and silver nanoparticles conjugated with heparin derivative possess anti-angiogenesis properties. Nanotech 2009; 20455104
Mohan YM, Joseph D, Geckeler K. Poly (n‐isopropylacrylamide‐co‐sodium acrylate) hydrogels: Interactions with surfactants. J Appl Polym Sci 2007; 103: 3423-30.
Kora AJ, Sashidhar R, Arunachalam J. Gum kondagogu (cochlospermum gossypium): A template for the green synthesis and stabilization of silver nanoparticles with antibacterial application. Carbohydr Polym 2010; 82: 670-9.
Gils PS, Ray D, Sahoo PK. Designing of silver nanoparticles in gum arabic based semi-ipn hydrogel. Int J Biol Macromol 2010; 46: 237-44.
Raveendran P, Fu J, Wallen SL. Completely “green” synthesis and stabilization of metal nanoparticles. J Am Chem Soc 2003; 125: 13940-1.
Ravindran A, Mani V, Chandrasekaran N, Mukherjee A. Selective colorimetric sensing of cysteine in aqueous solutions using silver nanoparticles in the presence of cr 3+. Talanta 2011; 85: 533-40.
Su H, Fan H, Ai S, et al. Selective determination of melamine in milk samples using 3-mercapto-1-propanesulfonate-modified gold nanoparticles as colorimetric probe. Talanta 2011; 85: 1338-43.
Wang G-L, Zhu X-Y, Jiao H-J, Dong Y-M, Li Z-J. Ultrasensitive and dual functional colorimetric sensors for mercury (ii) ions and hydrogen peroxide based on catalytic reduction property of silver nanoparticles. Biosens Bioelectron 2012; 31: 337-42.
Wang L, Liu X, Hu X, Song S, Fan C. Unmodified gold nanoparticles as a colorimetric probe for potassium DNA aptamers. Chem Commun 2006; 36: 3780-2.
Lee J-S, Lytton-Jean AK, Hurst SJ, Mirkin CA. Silver nanoparticle-oligonucleotide conjugates based on DNA with triple cyclic disulfide moieties. Nano Lett 2007; 7: 2112-5.
Warneck P. Chemistry of the natural atmosphere. 2nd ed. San Diego: Academic Press 1999.
Durbin TD, Wilson RD, Norbeck JM, Miller JW, Huai T, Rhee SH. Estimates of the emission rates of ammonia from light-duty vehicles using standard chassis dynamometer test cycles. Atmos Environ 2002; 36: 1475-82.
Pijolat C, Pupier C, Sauvan M, Tournier G, Lalauze R. Gas detection for automotive pollution control. Sens Actuators B 1999; 59: 195-202.
Ament W, Huizenga J, Kort E, Van Der Mark T, Grevink R, Verkerke G. Respiratory ammonia output and blood ammonia concentration during incremental exercise. Int J Sports Med 1999; 20: 71-7.
Narasimhan L, Goodman W, Patel CKN. Correlation of breath ammonia with blood urea nitrogen and creatinine during hemodialysis. Proc Natl Acad Sci 2001; 98: 4617-21.
Eiceman GA, Salazar MR, Rodriguez MR, et al. Ion mobility spectrometry of hydrazine, monomethylhydrazine, and ammonia in air with 5-nonanone reagent gas. Anal Chem 1993; 65: 1696-702.
Lähdesmäki I, Lewenstam A, Ivaska A. A polypyrrole-based amperometric ammonia sensor. Talanta 1996; 43: 125-34.
Li RC, Chan PC, Cheung PW. Analysis of a mos integrated gas sensor using a surface chemistry based model. Sens Actuators B 1995; 28: 233-42.
Jin Z, Su Y, Duan Y. An improved optical ph sensor based on polyaniline. Sens Actuators B 2000; 71118-22.
Onida B, Fiorilli S, Borello L, Viscardi G, Macquarrie D, Garrone E. Mechanism of the optical response of mesoporous silica impregnated with reichardt’s dye to nh3 and other gases. The J Phys Chem B 2004; 108: 16617-20.
Webber ME, Baer DS, Hanson RK. Ammonia monitoring near 1.5 µm with diode-laser absorption sensors. Appl Opt 2001; 40: 2031-42.
Mader HS, Wolfbeis OS. Optical ammonia sensor based on upconverting luminescent nanoparticles. Anal Chem 2010; 82: 5002-4.
Abaker M, Umar A, Baskoutas S, et al. A highly sensitive ammonia chemical sensor based on α-fe2o3 nanoellipsoids. J Phys D Appl Phys 2011; 44425401
Duncan TV. Applications of nanotechnology in food packaging and food safety: barrier materials, antimicrobials and sensors. J Colloid Interface Sci 2011; 363: 1-24.
Rahman MM, Jamal A, Khan SB, Faisal M. Characterization and applications of as-grown β-fe2o3 nanoparticles prepared by hydrothermal method. J Nanopart Res 2011; 13: 3789-99.
Vogel AI, Svehla G. Vogel’s qualitative inorganic analysis. Harlow: Longman 1996.
Martin JD, Telgmann L, Metcalfe CD. A method for preparing silver nanoparticle suspensions in bulk for ecotoxicity testing and ecological risk assessment. Bull Environ Contam Toxicol 2017; 98: 589-94.
Khalid Mustafa Osman O, Manal Ahmed GA, Awatif AH, Abeer Ramadan MA, Ahmed Sameer AH, Abdulhakeem AA. Synthesis of silver nanoparticles using fungi. US9701552B1 2017.
Manal Ahmed GA, Ebtesam MA, Sarah Saleh AA, Muzzammil IS, Manal FE. Synthesis of metal nanoparticles using an extract of terfeziacea. US9637807B1. 2016.
Slistan-Grijalva A, Herrera-Urbina R, Rivas-Silva J, Avalos-Borja M, Castillón-Barraza F, Posada-Amarillas A. Synthesis of silver nanoparticles in a polyvinylpyrrolidone (pvp) paste, and their optical properties in a film and in ethylene glycol. Mater Res Bull 2008; 43: 90-6.
Bin Ahmad M, Lim JJ, Shameli K, Ibrahim NA, Tay MY. Synthesis of silver nanoparticles in chitosan, gelatin and chitosan/gelatin bionanocomposites by a chemical reducing agent and their characterization. Molecules 2011; 16: 7237-48.
Solomon MM, Gerengi H, Umoren SA, Essien NB, Essien UB, Kaya E. Gum arabic-silver nanoparticles composite as a green anticorrosive formulation for steel corrosion in strong acid media. Carbohydr Polym 2018; 181: 43-55.
Williams PA, Phillips GO. GUMS | Properties of Individual Gums In: Caballero B, Eds Encyclopedia of food sciences and nutrition Oxford: Academic Press. 2003; p. pp. 2992-3001.
Elemike EE, Onwudiwe DC, Arijeh O, Nwankwo HU. Plant-mediated biosynthesis of silver nanoparticles by leaf extracts of Lasienthra africanum and a study of the influence of kinetic parameters. Bull Mater Sci 2017; 40(1): 129-37.
Mohan YM, Raju KM, Sambasivudu K, Singh S, Sreedhar B. Preparation of acacia‐stabilized silver nanoparticles: A green approach. J Appl Polym Sci 2007; 106(5): 3375-81.
Amirmostafa A. Davoudi. Colorimetric detection of ammonia using smartphones based on localized surface plasmon resonance of silver nanoparticles. Talanta 2018; 176: 242-6.
Carley A, Gwin ABC, Emilie L, Christina LA, Claudia KG. Microbial community response to silver nanoparticles and Ag+ in nitrifying activated sludge revealed by ion semiconductor sequencing. Sci Total Environ 2018; 616-617: 1014-21.
Kumar A, Aerry S, Goia DV. Preparation of concentrated stable dispersions of uniform Ag nanoparticles using resorcinol as reductant. J Colloid Interface Sci 2016; 470: 196-203.
Jonathan DM, Lena T, Chris DM. A method for preparing silver nanoparticle suspensions in bulk for ecotoxicity testing and ecological risk assessment. Bull Environ Contam Toxicol 2017; 98(5): 589-94.
Carley AG, Emilie L, Christina LA, Claudia KG. Microbial community response to silver nanoparticles and Ag+ in nitrifying activated sludge revealed by ion semiconductor sequencing. Sci Total Environ 2018; 616-617: 1014-21.
Solomon MM, Gerengi H, Umoren SA, Essien NB, Essien UB, Kaya E. Gum Arabic-silver nanoparticles composite as a green anticorrosive formulation for steel corrosion in strong acid media. Carbohydr Polym 2018; 181: 43-55.
Lodeiro P, Achterberg EP, Rey-Castro C, El-Shahawi MS. Effect of polymer coating composition on the aggregation rates of Ag nanoparticles in NaCl solutions and seawaters. Sci Total Environ 2018; 631-632: 1153-62.
Barsotti RJ. Measurement of ammonia in blood. J Pediatr 2001; 138: S11.
Chernecky CC, Berger BJ. Laboratory tests and diagnostic procedures. 6th ed. St Louis, MO 2013.
Ge L, Li Q, Wang M, Ouyang J, Li X, Xing MM. Nanosilver particles in medical applications: synthesis, performance, and toxicity. Int J Nanomedicine 2014; 9: 2399.
Jiang J, Oberdörster G, Biswas P. Characterization of size, surface charge, and agglomeration state of nanoparticle dispersions for toxicological studies. J Nanopart Res 2009; 11(1): 77-89.
Zhang Y, Li Y, Wei Q, Yan T, Du B, Hu L. A method of preparing ammonia gas sensor based on a noble metal compound in the construction. CN104502415B 2015.
Ankamwar B, Mandal G, Sur UK, Ganguly T. An effective biogenic protocol for room temperature one step synthesis of defective nanocrystalline silver nanobuns using leaf extract. Digest J Nano Biostruc 2012; 7: 599-605.

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